A Programmable In Vivo CRISPR Activation Model Elucidates the Oncogenic and Immunosuppressive Functions of MYC in Lung Adenocarcinoma.

Unlabelled: Conventional genetically engineered mouse models (GEMM) are time-consuming, laborious, and offer limited spatiotemporal control. Here, we describe the development of a streamlined platform for in vivo gene activation using CRISPR activation (CRISPRa) technology. Unlike conventional GEMMs, this model system allows for flexible, sustained, and timed activation of one or more target genes using single or pooled lentiviral guides.

Experience in Strategic Networking to Promote Palliative Care in a Clinical Academic Setting in India.

Background: Palliative care in low and middle-income countries is a new discipline, responding to a greater patient need, than in high-income countries. By its very nature, palliative as a specialty has to network with other specialties to provide quality care to patients. For any medical discipline to grow as a specialty, it should be well established in the teaching medical institutions of that country.

Palliative Sedation in Advanced Cancer Patients: Does it Shorten Survival Time? - A Systematic Review.

Background: Patients with advanced cancer often suffer from multiple refractory symptoms in the terminal phase of their life. Palliative sedation is one of the few ways to relieve this refractory suffering.

Objectives: This systematic review investigated the effect of palliative sedation on survival time in terminally ill cancer patients.

Palliative sedation at home.

Patients with advanced cancer often suffer from multiple intractable physical symptoms. Though majority of the symptoms can be controlled, in some of the patients these symptoms remain refractory and uncontrolled till the end. Palliative sedation (PS) is one of the ways to relieve intractable suffering of the dying cancer patients.

Oral morphine prescribing practices in severe cancer pain.

Background: Nearly one million cancer patients in India need oral morphine for pain relief. Despite doctors prescribing oral morphine in our center, many cancer patients with severe pain found to be not facilitated with adequate pain relief.

Aim: This audit was conducted to look at the "oral morphine prescribing practices for severe cancer pain" at a tertiary care hospital.

Nucleus accumbens lesion does not affect schedule induced polydipsia.

Rats with lesion of nucleus accumbens were hypodipsic under free-feeding conditions. In schedule-induced polydipsia (SIP) tests conducted by reducing the rats to 70% of free-feeding body weight and delivering 60 mg bengal gram pellets on a fixed time 1-min schedule, nucleus accumbens lesions did not delay the acquisition or show a decrease in the maintenance of S.I.