mRNA stability and mA are major determinants of subcellular mRNA localization in neurons.

For cells to perform their biological functions, they need to adopt specific shapes and form functionally distinct subcellular compartments. This is achieved in part via an asymmetric distribution of mRNAs within cells. Currently, the main model of mRNA localization involves specific sequences called "zipcodes" that direct mRNAs to their proper locations.

Massively parallel identification of mRNA localization elements in primary cortical neurons.

Cells adopt highly polarized shapes and form distinct subcellular compartments in many cases due to the localization of many mRNAs to specific areas, where they are translated into proteins with local functions. This mRNA localization is mediated by specific cis-regulatory elements in mRNAs, commonly called 'zipcodes'. Although there are hundreds of localized mRNAs, only a few zipcodes have been characterized.

Multi-Omics Alleviates the Limitations of Panel Sequencing for Cancer Drug Response Prediction.

Comprehensive genomic profiling using cancer gene panels has been shown to improve treatment options for a variety of cancer types. However, genomic aberrations detected via such gene panels do not necessarily serve as strong predictors of drug sensitivity. In this study, using pharmacogenomics datasets of cell lines, patient-derived xenografts, and ex vivo treated fresh tumor specimens, we demonstrate that utilizing the transcriptome on top of gene panel features substantially improves drug response prediction performance in cancer.

Identifying tumor cells at the single-cell level using machine learning.

Tumors are complex tissues of cancerous cells surrounded by a heterogeneous cellular microenvironment with which they interact. Single-cell sequencing enables molecular characterization of single cells within the tumor. However, cell annotation-the assignment of cell type or cell state to each sequenced cell-is a challenge, especially identifying tumor cells within single-cell or spatial sequencing experiments.

European Registry on the management of Helicobacter pylori infection (Hp-EuReg): analysis of 2360 patients receiving first-line therapy in Russia.

Aim: European Registry on the management of Helicobacter pylori infection («Hp-EuReg») - a multicenter prospective observational study initiated by the European Helicobacter and Microbiota Study Group, conducted in 27 European countries in order to evaluate the real clinical practice of diagnosis and treatment of H. pylori and its comparison with international recommendations.

Materials And Methods: The analysis of 2360 patients entered in the register by the Russian centres of «Hp-EuReg» in 2013-2017, who were underwent 1st line eradication therapy.

[Enteral dysfunction as a factor of protein-energy insufficiency development in elderly persons following gastric surgery].

264 patients within the age group of 61-93 were observed both by the gastroenterologist and surgeon for 3-6 years following gastroduodenal ulcer surgical treatment. 31,8% developed protein-energy insufficiency (PEI) in late terms, i.e.

[New approaches to assessment of military personnel health status].

For the first time were suggested some indices such as the index of ratio of one unit's (higher/highest formation) hospitalization, lost worktime, discharge and mortality to the primary morbidity for one military unit and the same index of ratio to the same criteria for the group of military unit. The mentioned peculiarities are intended for impartial and comprehensive estimation of Armed Forces of the Russian Federation military personnel health status and medical units (establishments) activity. These indices include as criteria of diseases prevalence, morbidity, hospitalization, discharge and mortality characteristics.

[Opportunities of nutritional therapy and typical errors in the nutrition of patients with biliary tract diseases].

This lecture intended for doctor describes the possibility to influence by nutrition the main mechanisms of pathogenesis in biliary tract diseases including cholestatic syndrome, disorders of bile physical-chemical properties, inflammation, biliary tract and intestinal disorders of motility. Typical mistakes in nutrition of patients with biliary tract pathology are discussed using clinical case history as an example.

[The clinical immunologic characteristics of different variants of course of ulcer colitis].

The study was carried out to determine clinical and immunologic predictors of unfavorable variant of course of ulcer colitis. The sample included 89 patients (48 females--53.9% and 41 males--46.

[A new system of specialized gastroenterological care to patients with inflammatory bowel diseases in St. Petersburg].

Aim: To substantiate the effectiveness of the set-up center in the early detection of patients with inflammatory bowel diseases (IBD) and in its organization and implementation of current therapeutic programs.

Materials And Methods: The therapeutic activity of the specialized medical care system set up in St. Petersburg for patients with IBD (ulcerative colitis (UC) and Crohn's disease (CD)), which is based on a multifunctional inflammatory bowel disease center at City Clinical Hospital Thirty-One, was analyzed.

[Gerontodietology].

Gerontodietology is one of the most important parts of dietetics studying problems of nutrition of elderly and old persons and persons with high risk of early and pathological aging, developing principles of rational nutrition, prophylactic and curative nutrition and methods of its organization. Functional structure of modern gerontodietology, its scientific-methodical bases, terminological features are presented. Composition and features of healthy nutrition of elderly people is described in details, methods of its optimization and individualization are given.

[Principles and perspectives of primary biliary cirrhosis therapy].

A survey of existing methods of pathogenetic and symptomatic treatment of primary biliary cirrhosis has been made and it has reviewed prospective directions of its therapy. Is also presents a retrospective analysis of our own data of S-adenosylmethionine (Heptral) in 27 patients PBC receiving UDCA basic therapy. In its application intravenously of 400 mg 2 times daily for 3 weeks showed a reduction in the severity of pruritus, assessed by visual analogue scale, a subjective decrease fatigability and a decrease level of serum bilirubin.

[Dietary prevention of protein-energy malnutrition during early postoperative period in elderly patients with gastroduodenal diseases].

The modified diet of postoperative rehabilitation program in elderly patients with gastroduodenal ulcers and prognosis of development of protein-energy malnutrition (PEM) is presented. It is shown that early initiated special diet in postoperative period, blocks mechanisms of malnutrition and can significantly improve the functional status of the small intestine and activate, thus, membrane digestion, which leads to normalization of all types of metabolism in elderly patients. In comparison with control group, where 72% of patients in postoperative period had malnutrition, malnutrition in the study group revealed a mild degree in only 17.

[Nutrition mistakes in patients with nonalcoholic fatty liver disease and the ways of correction].

Unlabelled: THE AIM OF REVIEW: To verify the role of nutrition in development of Nonalcoholic Fatty Liver Disease in patients with metabolic syndrome.

Original Positions: The article contains main conceptions of etiology and pathogenesis of Nonalcoholic Fatty Liver Disease. Spesial attention gives to the role of nutrition in development of this case.

[Modern dietetics on the new step development of medicine (clinical essay)].

Unlabelled: THE AIM OF REVIEW: To discuss some controversy moments in terminology. To show the possibility of modern dietetics in prophylacxis and treatment of deseases.

Original Positions: Dietetics is an impotent part of modern treatment, that is considered on exemple of endoecology and gerontal diet.

[Late diagnosis of autoimmune liver disease: causes and consequences].

The article is devoted to the problems of the well-timed diagnosis of autoimmune liver diseases, such as autoimmune hepatitis, primary biliary cirrhosis, primary sclerosing cholangitis and overlap-syndromes. It has been shown that diagnosis verification influences on the course of these conditions and the patient's longevity. The causes of last diagnosis and clinical and laboratory data have been analyzed.

[The synthesis of 2alpha,3alpha-isopropylidenedioxy-6,6-ethylenedioxy-5alpha-androst-15-en-17-one and its 2beta,3beta-isomer].

Androstane and delta15-androstane analogues of brassinosteroids were synthesized from dehydroepiandrosterone. The key stage, hydroxylation of 17beta-acetoxyandrost-2-en-6-one double bond with OsO4, yielded the corresponding 2alpha,3alpha- and 2beta,3beta-diols. The target 2alpha,3alpha-isopropylidenedioxy-6,6-ethylenedioxy-5alpha-androst-15-en-17-one and its 2beta,3beta-isomer were obtained by dehydrosilylation of the corresponding silylene ethers with palladium acetate.

[DNA-hydrolyzing IgG antibodies from the blood of patients with acquired immune deficiency syndrome].

DNAase activity of 110 samples of IgG from the blood of AIDS patients was analyzed. It was shown that the relative activity of preparations varies very much from patient to patient, but 96% preparations show detectable level of DNAase activity. Several rigid criteria were applied and it was shown that DNAase activity is an intrinsic property of antibodies from AIDS patients.

Proteolytic activity of IgG antibodies from blood of acquired immunodeficiency syndrome patients.

Proteolytic activity of polyclonal IgG antibodies (Abs) from the blood of AIDS patients was analyzed for the first time. These Abs were shown to display higher activity in hydrolysis of beta-casein than in hydrolysis of human immunodeficiency virus (HIV)-1 reverse transcriptase (RT) or human serum albumin (HSA). Several abzymatic criteria were applied and it was shown that RT, HSA, and beta-casein hydrolyzing activities are an intrinsic property of polyclonal Abs from AIDS patients.

[Neuroendocrinology of the stomach and aging].

The survey contains information about basic stomach endocrine cells in normal condition and their changes in chronic atrophic gastritis and ageing. The data of modern research devoted to endocrine cells age-related changes are represented here and analyzed. The reasons that influence on endocrine cells age-related changes and the ways of their possible correction are described.

Comparison of enzymatic properties of DNA-abzymes and human DNAses.

DNA-hydrolyzing antibodies (DNA-abzymes, Abz) were shown to be good biochemical markers of some autoimmune diseases such as systemic lupus erythematosus (SLE) and multiple sclerosis (MS). To better understand mechanisms of abzyme generation, one needs to know optimal conditions for DNA hydrolysis by DNA-abzymes, as well as their enzymatic properties in comparison with those of enzymes possessing the same activity. In contrast to human urine deoxyribonucleases, DNA-hydrolyzing antibodies efficiently digested both single- and double-strand DNA.

Human deoxyribonucleases.

Although mammalian deoxyribonucleases were discovered more than 60 years ago, interest in these enzymes is not weakening. During the last decade, intensive studies of human DNases culminated in discovery of several novel enzymes exhibiting DNase activity. These include an unusual DNase, lactoferrin.

[Characteristic of combat trauma and treatment of gunshot fractures of long bones of the limbs].

Treatment of the patients with gunshot fractures of extremity long bones is one of the main problems of field surgery. The complex study of medical records obtained from 718 servicemen with gunshot fractures of extremity long bones who participated in counter-terrorist operation in the Republic of Chechnia (1994-1996) allowed to evaluate character and severity of the injuries, content of the treatment measures conducted at the stages of medical evacuation. The peculiarities of fighting trauma were the following: the high frequency of combined (22.