Neonatal screening of ABO/RhD blood group and direct antiglobulin Coombs test. Experience of a single institution.

Background: Evaluating the ABO/RhD blood group and the direct antiglobulin Coombs test (DAT) at birth is recommended good practice, but there is variability in its universal implementation. This study aims to show the comparative results in various variables of clinical impact during the hospital stay of neonates with positive DAT compared with those with negative DAT, based on the systematic detection of the ABO/RhD group and DAT at birth.

Methods: Newborns between 2017 and 2020 in a high-risk pregnancy care hospital were included.

Use of computational biology to compare the theoretical tertiary structures of the most common forms of RhCE and RhD.

Background And Objectives: Computational biology analyses the theoretical tertiary structure of proteins and identifies the 'topological' differences between RhD and RhCE. Our aim was to identify the theoretical structural differences between the four isoforms of RhCE and RhD using computational biological tools.

Materials And Methods: Physicochemical profile was determined by hydrophobicity and electrostatic potential analysis.

Analysis of a Novel Mexican Variant of the Gene Associated with β-Thalassemia Using Bioinformatic Tools.

We present a study performed on 54 unrelated subjects, with and without thalassemic features. Two primer pairs were proposed to perform Sanger sequencing of the complete gene. The bioinformatic analysis was performed taking advantage of the availability of free online tools.

Low prevalence of IgG antibodies against antigens of HEV genotypes 1 and 3 in women with a high-risk pregnancy.

The aim of the study was to assess whether high-risk pregnant women have a higher prevalence of HEV during the perinatal period. This was a cross-sectional study of 428 patients: Group 1, 127 women with a high-risk pregnancy; Group 2, 97 asymptomatic people with reactivity to HCV or HBV; Group 3, 94 patients with clinical symptoms suggestive of HEV infection; and Group 4, 110 healthy blood donors from an urban area of Mexico City. ELISA was used to measure antibody to HEV genotypes 1 and 3.

Clara Cell Protein Expression in Mechanically Ventilated Term and Preterm Infants with Respiratory Distress Syndrome and at Risk of Bronchopulmonary Dysplasia: A Pilot Study.

The aim of this pilot study was to determine Clara cell protein (CC16) concentration in bronchoalveolar lavages (BAL) fluid from full-term and preterm (<37 weeks' gestational age) neonates requiring respiratory support, having symptoms of neonatal respiratory distress syndrome, and at risk of bronchopulmonary dysplasia (BPD). We hypothesized that CC16 may be predictive of BPD diagnosis regardless of gestational age. BAL fluid CC16 was measured by ELISA at birth and at day 7 of life.

[Molecular identification of Hemoglobin D Punjab in cases detected in two families].

Background: Hemoglobin D Punjab is the world most common variant hemoglobin D; in Mexico there are reports of isolated cases. Our goal is to present the clinical and molecular study in two families with HbD Punjab. The objective was to submit molecular diagnosis of two families with Hb D Punjab and clinical features.

Frequency of hepatitis C virus infection in a single institution in Mexico with a focus on birth-cohort population.

Unlabelled:  Background and rationale for the study. The generation of people born before 1965 is a high-risk group for developing chronic hepatitis C virus (HCV) infection.

Aim: To report the experience on single institution of HCV infection under birth-cohort or baby boomers effect.

Estimation of umbilical cord blood leptin and insulin based on anthropometric data by means of artificial neural network approach: identifying key maternal and neonatal factors.

Background: Leptin and insulin levels are key factors regulating fetal and neonatal energy homeostasis, development and growth. Both biomarkers are used as predictors of weight gain and obesity during infancy. There are currently no prediction algorithms for cord blood (UCB) hormone levels using Artificial Neural Networks (ANN) that have been directly trained with anthropometric maternal and neonatal data, from neonates exposed to distinct metabolic environments during pregnancy (obese with or without gestational diabetes mellitus or lean women).

[Molecular identification of glucose-6-phosphate dehydrogenase (G6PD) detected in neonatal screening].

Aims: To present the strategy of identifying the molecular variants of G6PD detected in neonatal screening (NS).

Material And Methods: We present a series of incident cases of newborns positive for G6PD deficiency detected in NS. From nuclear DNA with the methodology of real-time PCR we sought molecular G6PD variants: G202A, A376G, T968C and C563T.

[Acute adverse effects in transfusion. Proposals for the hemosurveillance system].

The management model based on risk prevention has become a major influence in shaping policies for transfusion safety. There are approximately sixty interactions between the health worker and the patient during the transfusion process,representing the number of times where you have the opportunity to make a mistake.We present an analysis of the weaknesses of the National Blood System, with particular attention to the haemovigilance donor and patient.

Association between genotype and plasma levels of thrombin-activated fibrinolysis inhibitor (TAFI) in the development of preeclampsia.

Introduction: The objective was to evaluate if thrombin-activated fibrinolysis inhibitor (TAFI) polymorphisms (G505A, C1040T, and G-438A), and TAFIa plasma levels are associated with preeclampsia.

Materials And Methods: In a case-control study design, we evaluated preeclampsia patients and women with uncomplicated pregnancies. The TAFI polymorphisms were determined by real-time PCR method, and TAFIa plasma levels were established with a chromogenic assay.

[RH hybrid box identification in subjects with Rh negative phenotype from Mexico's Valley].

Background: The prevalence of the RhD and RhCE gene alleles is related to the ethnic mixture. The aim of this report is to describe the predominant molecular mechanisms in RhD negative subjects residents from Mexico's valley according to the phenotype of RhCE.

Methods: Blood samples from RhD negative women and men were studied.

Hepatic expression of ghrelin and adiponectin and their receptors in patients with nonalcoholic fatty liver disease.

Background & Aim: Adiponectin and ghrelin are hormones that participate in hepatic lipid metabolism, and their expression in liver tissue could have important implications for nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the hepatic expression of ghrelin, adiponectin, AdipoR, and IL-6 in patients with NAFLD and normal liver.

Methods: We studied patients with clinical-pathological diagnosis of NAFLD or a normal liver.

Importance of iron and iron metabolism in nonalcoholic fatty liver disease.

Iron homeostasis disturbances are associated with liver disease. Non-alcoholic steatohepatitis is part of the spectrum of non-alcoholic fatty liver disease, which can progress to hepatic cirrhosis and end-stage liver disease. Increasing information supports that multiple factors underlie the development and progression of nonalcoholic steatohepatitis.

Endocannabinoid receptor CB2 in nonalcoholic fatty liver disease.

Background And Aim: Fatty infiltration and fibrosis are major issues in chronic liver disease. Recent reports suggest a role for the endocannabinoid system in these processes.

Aim: To characterize localization and expression of CB2 in normal liver and nonalcoholic fatty liver.

Association of HFE mutations (C282Y and H63D) with iron overload in blood donors from Mexico City.

Background And Objective: Iron overload has been associated with HFE mutations (C282Y and H63D). We investigated the association between these mutations and high serum ferritin in a sample of healthy adult men.

Design And Methods: We enrolled unrelated blood donors from three hospitals in Mexico City in a crosssectional study.

Obesity-related leptin receptor polymorphisms and gallstones disease.

Objective: Investigate the association between polymorphisms in the leptin receptor gene associated with obesity and gallstone disease.

Design: We conducted a cross-sectional study, carried out at a tertiary setting.

Subjects: We enrolled 97 subjects, comprising 54 subjects with gallstones (cases) and 43 controls (without gallstones).

[Variations in serum ferritin and erythrocyte index in the first eight weeks of life in term newborn infants].

Objective: To describe changes in erythrocyte index during the first 8 weeks of life in neonates in relation to their iron store.

Material And Methods: We performed a longitudinal study of a group of healthy term newborn infants, in whom we evaluated erythrocyte index and serum ferritin (SF) values at birth and at weeks 4 and 8 of age. Depending on the comparison made in SF values between birth and 2 months, the infants were divided into two groups: group I (without variation in SF) and group II (with a decrease in SF).

Evaluation of iron overload in healthy adult residents of Mexico City.

Background: We described the effects of age, gender and body mass index (BMI) on the prevalence of iron overload (IO) in blood donors from Mexico City.

Methods: A cross-sectional study of clinically healthy adults was performed. We evaluated serum ferritin (SF) concentration to allow us to establish groups with normal iron stores (SF >30 microg/L) and with IO (SF >200 microg/L and >300 microg/L for women and men), in the following ages groups: 18-29 years, 30-49 years, and 50-64 years, divided by gender.