Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.

Detailed characterization of the gene expression patterns in spermatogonia and primary spermatocytes is critical to understand the processes which occur prior to meiosis during normal spermatogenesis. The genome-wide expression profiles of mouse type B spermatogonia and primary spermatocytes were investigated using the Solexa/Illumina digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental processes which occur during early spermatogenesis were systematically analyzed. Gene expression patterns vary significantly between mouse type B spermatogonia and primary spermatocytes.

[Study on preparation and quality standard of Xueshuantong Drop Pills].

Objective: To optimize the best technical parameters and establish the quality standard for Xueshuantong Drop Pills.

Methods: Using moulding probability of drop pill as the evaluation to choose the best dropping condition. Using parallel experimentation for the choice of proportion of drug and matrix, and refrigerant; HPLC method was employed on ODS column which was used with a mobile phase of acetontrile-water, 0 min 20% A, 14 min 40% A, 15 min 60% A, 16 min 20% A.

Prokaryotic expression and characterization of human AC3-33 protein.

The transcription factor, AP-1, plays an important role in cellular proliferation, transformation and death. We previously showed that AC3-33 (GenBank name: c3orf33, FLJ31139), significantly inhibited transcriptional activity of AP-1. In this study, we report a method to express and purify AC3-33 in E.

The role of P2X7 receptor in ATP-mediated human leukemia cell death: calcium influx-independent.

Activation of the P2X7 receptor leads to a rapid, bidirectional flux of cations, causing broad range of biological responses including cytotoxicity. However, the mechanism of P2X7-mediated cytotoxicity remains largely unexplored. In our previous study, the lack of P2X7-mediated calcium response under normal conditions was found in P2X7(+) hematopoietic cell lines.

[Preparation of new lipid-hydroxyapatite-DNA complex and gene transfection reseach in eukaryotic cell].

This work was directed at obtaining a better gene carrier to improve the effects of gene delivery. Neutral liposomes made from cholesterol, lecithin and DOPE by reverse evaporation technique were used for encapsulating DNA-HAP complex which was made from DNA and optimized HAP. The sizes of complexes and the efficiency of encapsulation were detected.

[Human parasitology teaching in the 21st century].

Multimedia techniques were applied in parasitological teaching and experimental practices. In order to strengthen the practical ability of the undergraduate students, reform was conducted including a prioritization of the teaching content, use of series micro-slides and video show, etc.