One-step biomineralization to synthesize reusable CRL@ZnCo-MOF for boosting lipase stability and sustainable dibutyl phthalate removal.

Adsorption and biodegradation are two important means to remove the pollutants from the environment, but how to combine them and improve the catalytic performance and stability of free enzyme are facing great challenges. Herein, lipase from Candida rugosa (CRL) was immobilized into bimetallic ZnCo-MOF by biomineralization, which not only significantly improved the catalytic activity and stability of CRL but also endowed it with excellent reusability. Furthermore, CRL@ZnCo-MOF established a synergetic system of combined adsorption and enzymatic degradation for the sustainable removal of dibutyl phthalate (DBP) in actual water environment.

Removal of phthalate esters by integrated adsorption and biodegradation using improved performance of lipase@MOFs.

Phthalate esters (PAEs) are broadly utilized as plasticizers in industrial products, posing a significant threat to ecological security and human health. Lipase is a kind of green biocatalyst with the ability to degrade PAEs, but its application is limited due to its low stability and poor reusability. Herein, lipase from Candida rugosa (CRL) was immobilized into an organic ligand replacement MOFs (MAF-507) and cysteine modification and glutaraldehyde cross-linking were simultaneously performed to synthesize immobilized lipase (Cys-CRL@GA@MAF-507) using a one-pot method.

Creative art therapy for postpartum depression： A systematic review and meta-analysis.

Objective: This study aims to ascertain the effectiveness of creative art therapy (CAT) in alleviating symptoms of postpartum depression (PPD).

Methods: A systematic review of all randomized controlled trials of CAT for PPD was conducted. Eight bibliographic databases, namely, PubMed, The Cochrane Library, Embase, Web of Science, CNKI, Wanfang Database, VIP Database, and China Biomedical Literature Database were searched digitally, while Google Scholar database was searched manually for a comprehensive literature search.

Green Fluorescent Carbon Dots with Critically Controlled Surface States: Make Silk Shine via Feeding Silkworms.

Feeding silkworms with functional materials as additives to produce naturally modified silk is a facile, diverse, controllable, and environmentally friendly method with a low cost of time and investment. Among various additives, carbon dots (CDs) show unique advantages due to their excellent biocompatibility and fluorescence stability. Here, a new type of green fluorescent carbon dots (G-CDs) is synthesized with a high oil-water partition ratio of 147, a low isoelectric point of 5.

The correlation between menstrual characteristics and fertility in women of reproductive age: a systematic review and meta-analysis.

Importance: Menstruation serves as an indicator of women's reproductive well-being and plays a pivotal role in their fertility; nevertheless, there remains an ongoing debate regarding the epidemiological evidence linking menstrual characteristics as well as fertility.

Objective: To explore the correlation between menstrual characteristics and fertility in women of reproductive age.

Data Sources: A comprehensive literature search was conducted using PubMed, Embase, Web of Science, and Cochrane libraries to identify research articles published up until February 9, 2024.

Correction: A poly(thymine)-templated fluorescent copper nanoparticle hydrogel-based visual and portable strategy for an organophosphorus pesticide assay.

Correction for 'A poly(thymine)-templated fluorescent copper nanoparticle hydrogel-based visual and portable strategy for an organophosphorus pesticide assay' by Jihua Chen , , 2019, , 2423-2429, https://doi.org/10.1039/C9AN00017H.

Dual-functional cellulase-mediated gold nanoclusters for ascorbic acid detection and fluorescence bacterial imaging.

Protein-protected metal nanomaterials are becoming the most promising fluorescent nanomaterials for biosensing, bioimaging, and therapeutic applications due to their obvious fluorescent molecular properties, favorable biocompatibility and excellent physicochemical properties. Herein, we pioneeringly prepared a cellulase protected fluorescent gold nanoclusters (Cel-Au NCs) exhibiting red fluorescence under the excitation wavelength of 560 nm via a facile and green one-step method. Based on the fluorescence turn-off mechanism, the Cel-Au NCs were used as a biosensor for specificity determination of ascorbic acid (AA) at the emission of 680 nm, which exhibited satisfactory linearity over the range of 10-400 µM and the detection limit of 2.

Reusable carboxylesterase immobilized in ZIF for efficient degradation of chlorpyrifos in enviromental water.

The past few decades have witnessed biodegradation of pesticides as a significant method in remediation of the environment for its specificity, efficiency and biocompatibility. However, the tolerability and recyclability of the enzymes in pesticide degradation and the development of enzymes that biodegrad pesticides are still urgent problems to be solved so far. Herein, a novel hyper-thermostable and chlorpyrifos-hydrolyzing carboxylesterase EstC was immobilized by biomineralization using zeolitic imidazolate framework (ZIF), one of the metal-organic frameworks (MOFs) with highly diverse structure and porosity.

Recent progress of Bioinspired Hydrogel-based delivery system for endometrial repair.

Endometrial injury is the main fact leading to infertility. Current treatments of endometrial injury present many problems, such as unable to achieve desired effects due to low retention and the inherent potential risk of injury. Besides, it is important to the development of bioinspired material that can mimic the natural tissue and possess native tissue topography.

Acupuncture for Female Infertility: Discussion on Action Mechanism and Application.

A higher incidence of female infertility has been reported with an unexpectedly early appearance in recent years. The female infertility treatment and application of assisted reproductive technology have recently gained immense interest from scientists. Many studies have discussed the beneficial effects of acupuncture on female infertility.

Excellent catalytic properties of luminescent Cu@CuS nanozymes and their antibacterial applications.

Luminescent Cu@CuS nanozymes have been prepared by a one-pot method, displaying high peroxidase-like and oxidase-like activity. Fluorescence images show that these nanozymes adhere to the surface of the bacterial cell and scanning electron microscopy reveals that the nanomaterials cause folding and collapse of the bacterial surface, resulting in bacterial death.

Transcriptomic study of the mechanism by which the Kai Yu Zhong Yu recipe improves oocyte quality in a stressed mouse model.

Ethnopharmacological Relevance: The Kai Yu Zhong Yu recipe (KYZY) is a classic herbal formula in traditional Chinese medicine (TCM) that has been used to treat infertility associated with psychological stress for more than three hundred years.

Aim Of The Study: Psychological stress has major impacts on fertility, with variable outcomes depending on the nature, strength, and duration of the stress. Stress can directly disturb ovulation, oocyte quality, maturation, and embryo development.

Characterization of a carboxylesterase with hyper-thermostability and alkali-stability from Streptomyces lividans TK24.

A gene (estA', 804 bp) from Streptomyces lividans TK24 was artificially synthesized and successfully overexpressed as a 6His-tagged fusion protein in Escherichia coli. It encoded a carboxylesterase (EstA) that composed of 267 amino acids with a predicted molecular weight of 28.56 kDa.

Autonomous operation of 3D DNA walkers in living cells for microRNA imaging.

Three dimensional (3D) DNA walkers hold great potential in serving as an ideal candidate for signal transduction and amplification in bio-assays. However, the autonomous operation of 3D DNA walkers inside living cells is still few and far between, which could be attributed to the lack of suitable driving forces and moderate efficiency in terms of the cellular uptake of such complex 3D DNA components. Herein, a newly updated autonomously operated and highly integrated 3D DNA walker on Au nanoparticles (Au NPs)/zeolitic imidazolate framework-8 (ZIF-8) was activated in a tumor microenviroment and its signal amplified assay capability in living cells was demonstrated using miRNA as a sensing model biomolecule.

Characterization of a novel hyper-thermostable and chlorpyrifos-hydrolyzing carboxylesterase EstC: A representative of the new esterase family XIX.

Carboxylesterases have widely been used in a series of industrial applications, especially, the detoxification of pesticide residues. In the present study, EstC, a novel carboxylesterase from Streptomyces lividans TK24, was successfully heterogeneously expressed, purified and characterized. Phylogenetic analysis showed that EstC can be assigned as the first member of a novel family XIX.

Flash nanoprecipitation of ultra-small semiconducting polymer dots with size tunability.

Small-sized semiconducting polymer dots (Pdots) provide better tissue and subcellular penetration while minimizing unspecific interactions, and make the fast clearance of Pdots from human bodies possible by urinary excretion. We employ a powerful and scalable technology, flash nanoprecipitation, to prepare Pdots with small sizes (hydrodynamic diameters ∼10 nm).

Intracellular Imaging of Glutathione with MnO Nanosheet@Ru(bpy)-UiO-66 Nanocomposites.

Fluorescent detection of glutathione (GSH) in the living system has attracted much attention, but current fluorescent probes are usually exposed to the exterior environment, leading to photobleaching and premature leakage and subsequently limiting the sensitivity and photostability. Herein, luminescent metal-organic frameworks [Ru(bpy) encapsulated in UiO-66] coated with manganese dioxide nanosheets [MnO NS@Ru(bpy)-UiO-66] were prepared by an in situ growth method and further explored to construct a GSH-switched fluorescent sensing platform. Because of the splendid fluorescence quenching ability, special probe leakage blocking role and distinguished recognition of the MnO NS, and the improved fluorescence of Ru(bpy) by UiO-66, a low background, highly sensitive and selective detection of GSH with a low limit of detection as 0.

Fluorometric methods for determination of HO, glucose and cholesterol by using MnO nanosheets modified with 5-carboxyfluorescein.

A fluorescent probe for HO is described. It is composed of MnO nanosheets and 5-carboxyfluorescein and was characterized by fluorescence, transmission electron microscopy, ultraviolet-visible absorption spectra, energy dispersive X-ray and Fourier transform infrared spectroscopy. The probe, with fluorescence excitation/emission maxima at 490/518 nm, responds to HO in the 1 to 200 μM concentration range and has a 0.

PABPC1L depletion inhibits proliferation and migration via blockage of AKT pathway in human colorectal cancer cells.

Numerous studies have demonstrated that PABPC1 participates in the process of carcinogenesis and its function is inconsistent in different types of cancers. PABPC1-like (PABPC1L) is an important paralog of PABPC1 and few studies are available on the roles of PABPC1L in colorectal cancer (CRC) development. Hence, we explored the biological function and prognostic impact of PABPC1L in CRC.

A poly(thymine)-templated fluorescent copper nanoparticle hydrogel-based visual and portable strategy for an organophosphorus pesticide assay.

Since fluorescence assays with high sensitivity for organophosphorus pesticides (OPs) are urgently required to protect the ecosystem and prevent disease, an environmentally friendly and label-free fluorescent probe is desirable. Herein, a poly-thymine30 DNA-templated copper nanoparticle (poly T30-Cu NPs) hydrogel fluorescent probe was explored for the construction of an OPs sensing platform via tyrosinase (TYR) enzyme-controlled quenching. Initially, TYR can efficiently quench the fluorescence of poly T30-Cu NPs; however, when OPs are mixed with a certain amount of TYR, the fluorescence of poly T30-Cu NPs can be recovered.

Fe Foil-Guided Fabrication of Uniform Ag@AgX Nanowires for Sensitive Detection of Leukemia DNA.

Herein, we report a novel Fe foil-guided, in situ etching strategy for the preparation of highly uniform Ag@AgX (X = Cl, Br) nanowires (NWs) and applied the photoelectric-responsive materials for sensitive photoelectrochemical (PEC) detection of leukemia DNA. The Ag@AgX NW formation process was discussed from the redox potential and K value. The fabricated PEC platform for sensing leukemia DNA showed good assay performance with a wide linear range (0.

Aprotinin Encapsulated Gold Nanoclusters: A Fluorescent Bioprobe with Dynamic Nuclear Targeting and Selective Detection of Trypsin and Heavy Metal.

Fluorescence imaging has currently emerged as one of the most frequently used noninvasive imaging technologies to selectively monitor biological processes in living systems. In past decades, gold nanoclusters (Au NCs) has received increasing attraction because of their intrinsic fluorescence and their inherent biocompatibility. As a stabilizing and reducing agent, an abundant, sustainable, and widely used polypeptide derived drug molecule, aprotinin (Ap), is selected for the synthesis of Au nanoclusters (Ap-Au NCs) due to characteristic bioactivity, excellent biocompatibility, biodegradability, and non-allergenic character.

Portable Aptasensor of Aflatoxin B1 in Bread Based on a Personal Glucose Meter and DNA Walking Machine.

Despite some recent developments on the portable on-site sensor of Aflatoxin B1 (AFB1), the complex and expensive preparation of recognition elements have still limited their wide applications. In this paper, using the fast, low-cost, and stable recognition of aptamer DNA-AFB1, a portable aptasensor was constructed for the on-site detection of AFB1 in food matrixes, with the readout of personal glucose meter (PGM) and DNA walking machine for signal probe separation. In such an assay protocol, the target could trigger the DNA walker to autonomously move on the electrode surface, propelled by unidirectional Pb-specific DNAzyme digestion, which could amplify the signal and separate the signal probe as well for further quantification by the PGM.

Heyan Kuntai capsule versus dehydroepiandrosterone in treating Chinese patients with infertility caused by diminished ovarian reserve: a multicenter, randomized controlled trial.

Objective: To evaluate the clinical efficacy and safety of Heyan Kuntai capsule (HYKT) in treating women with infertility caused by diminished ovarian reserve (DOR).

Methods: One hundred eight eligible patients from three Chinese hospitals were randomly divided into an HYKT treatment group (n = 55) or a dehydroepiandrosterone (DHEA) treatment group (n = 53). Patients in the HYKT group were treated orally with four 0.