The Bacterial Mobile Resistome Transfer Network Connecting the Animal and Human Microbiomes.

Unlabelled: Horizontally acquired antibiotic resistance genes (ARGs) in bacteria are highly mobile and have been ranked as principal risk resistance determinants. However, the transfer network of the mobile resistome and the forces driving mobile ARG transfer are largely unknown. Here, we present the whole profile of the mobile resistome in 23,425 bacterial genomes and explore the effects of phylogeny and ecology on the recent transfer (≥99% nucleotide identity) of mobile ARGs.

MiR-449a regulates autophagy to inhibit silica-induced pulmonary fibrosis through targeting Bcl2.

Unlabelled: Silicosis is a fatal pulmonary fibrotic disorder characterized by accumulation of fibroblasts and myofibroblasts and deposition of extracellular matrix proteins. MiR-449a is a potential mediator of many cellular processes, including cell proliferation, differentiation, and apoptosis. We hypothesized that miR-449a may play a crucial role in the progression of pulmonary fibrogenesis.

Molecular Pathology of Pulmonary Edema in Forensic Autopsy Cases with Special Regard to Fatal Methamphetamine Intoxication.

Pulmonary edema is a common finding in fatal methamphetamine intoxication. However, the underlying mechanism remains poorly understood. This study investigated the molecular pathology of alveolar damage involving pulmonary edema in forensic autopsy cases.

An investigation report about the lack of VB1.

From August 1 to September 26, 2012, 30 workers who had the symptom of edema in both lower limbs were reported in Yancheng, Four severe edemas in both lower limbs workers were sent to the hospital, and one person of the workers died. The epidemiology investigation and laboratory testing (in the urine loading tests, the concentration of VB1 of the workers living in the factory for more than half of the year decreased far below the normal values) were conducted to explore the cause. Furthermore, after one-week intramuscular injection of thiamine treatment, the clinical symptoms of the workers had been improved greatly.

Association of rhizosphere soil microbiome with the occurrence and development of replant disease - A review.

Replant disease devastates crop growth, and its disease etiology has not been fully elucidated. The latest research revealed its close association with the changes of rhizosphere microecology, especially the changes in rhizosphere microbial community structure. With the advance of modern high-throughput DNA sequencing technology, the relationship between rhizosphere microbiome and replant diseases has become a popular research focus.

Genetic Variations in the Promoter of the APE1 Gene Are Associated with DMF-Induced Abnormal Liver Function: A Case-Control Study in a Chinese Population.

Acute or long-term exposure to N,N-dimethylformamide (DMF) can induce abnormal liver function. It is well known that DMF is mainly metabolized in the liver and thereby produces reactive oxygen species (ROS). The base excision repair (BER) pathway is regarded as a very important pathway involved in repairing ROS-induced DNA damage.

Dimethylacetamide-induced occupational toxic hepatitis with a short term recurrence: a rare case report.

In recent years, N,N-dimethylacetamide (DMAc) is widely used in the textile and plastics industry as a solvent alternative to more toxic N,N-dimethylformamide (DMF). At home and abroad, sporadic group case reports have revealed that DMAc could cause toxic hepatitis and symptoms or signs indicative of liver involvement among workers exposed to DMAc, with severe cases leading to death. This paper reports a rare case of severe acute toxic hepatitis with a short term recurrence induced by occupational contact with DMAc in a polyimide film factory, as well as in-depth analysis via relevant information and literature.

The Expression of B-Type Natriuretic Peptide After CaCl2-Induced Arrhythmias in Rats.

To investigate the patterns of B-type natriuretic peptide (BNP) expression after arrhythmia, BNP was assessed at different time points (0 minute, 10 minutes, 30 minutes, 1 hour, 3 hours, and 6 hours) in CaCl2-induced arrhythmia in rats through various methods such as immunohistochemistry, Western blotting, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay. Immunohistochemistry results showed that the expression of BNP in the endocardium was higher than that in the epicardium in rats undergoing sustained arrhythmias. The BNP-to-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) ratios determined by Western blotting analysis revealed no change at 0 minute but increased at 10 minutes and reached the first peak (0.

Investigation and Evaluation of Children's Blood Lead Levels around a Lead Battery Factory and Influencing Factors.

Lead pollution incidents have occurred frequently in mainland China, which has caused many lead poisoning incidents. This paper took a battery recycling factory as the subject, and focused on measuring the blood lead levels of environmental samples and all the children living around the factory, and analyzed the relationship between them. We collected blood samples from the surrounding residential area, as well as soil, water, vegetables.

Group and insidious tetraethyl lead poisoning occurred in industry of plastic weaving: a case report.

Tetraethyl lead (TEL) poisoning has declined sharply with decreasing consumption of gasoil and other chemicals contained TEL. Here we reported group TEL poisoning in the plastic weaving factory. We investigated 16 cases with the typical nerves disorder which is similar to organotin poisoning, and the result suggested that the poisoning may cause by applied "white oil" contented TEL.

Will testosterone replacement therapy become a new treatment of chronic heart failure? A review based on 8 clinical trials.

Background: According to the present evidences suggesting association between low testosterone level and prediction of reduced exercise capacity as well as poor clinical outcome in patients with heart failure, we sought to determine if testosterone replacement therapy (TRT) improves clinical and cardiovascular conditions as well as quality of life status in patients with stable chronic heart failure (CHF).

Methods: We carried out a review based on 8 published clinical trials to determine whether TRT will benefit patients with CHF. Information of exercise capacity, hemodynamic parameters, electrocardiogram indicators, muscle strength, echocardiography guidelines and laboratory indexes were collected to assess clinical outcomes.

Plasma microRNAs expression profile in female workers occupationally exposed to mercury.

Background: Circulating microRNAs (miRNAs) have attracted interests as non-invasive biomarkers of physiological and pathological conditions. Several studies have examined the potential effects of mercury exposure on miRNAs expression profiles of general population environmentally exposed to mercury. The objective is to identify mercury-related miRNAs of female workers occupationally exposed to mercury.

A multiplayer game: species of Clostridium, Acinetobacter, and Pseudomonas are responsible for the persistence of antibiotic resistance genes in manure-treated soils.

Antibiotics are routinely used in modern livestock farming. The manure from medicated animals is used for the fertilization of arable crops, which in turn leads to the accumulation of antibiotic resistance genes (ARGs) in the environment. This is a potentially serious public health issue, yet the identities of the bacterial taxa involved in ARG persistence are as yet undetermined.

Proteomic profiling differences in serum from silicosis and chronic bronchitis patients: a comparative analysis.

Background: Silicosis is a severe occupational disease characterized by pulmonary fibrosis, whereas chronic bronchitis (CB) is an acute inflammation of the airways. Differences in the mechanisms of pathogenesis of these diseases are not well understood, therefore we performed proteomic profiling of silicosis and CB patients and, compared the results.

Methods: Two-dimensional gel electrophoresis and MALDI-TOF-MS (matrix assisted laser desorption ionization time of flight mass spectrometry) were used to identify differentially accumulated proteins in stage I of silicosis (SI), stage II of silicosis (SII) and CB.

PON2 and ATP2B2 gene polymorphisms with noise-induced hearing loss.

Background: Noise-induced hearing loss (NIHL) is a complex disease induced by a combination of genetic and environmental factors. Paraoxonase2 (PON2) gene involved in the regulation of reactive oxygen species, and affecting the vulnerability of cochlea to NIHL, and ATPase, calcium-transporting, plasma membrane 2 (ATP2B2) gene which encodes plasma membrane calcium-transporting ATPase isoform 2 (PMCA2) are the candidate genes relating to the attack of NIHL. In this study, we investigated whether ATP2B2 and PON2 polymorphisms were associated with NIHL in Chinese of Han nationality population.

Prevalence of hypertension and noise-induced hearing loss in Chinese coal miners.

Background: Owing to inconsistent epidemiologic evidence and the presence of confounding factors, the relation between occupational noise exposure and hypertension still remained unclear. We aimed to assess whether Chinese coal miners were at risk of developing hypertension and noise induced hearing loss (NIHL), and whether occupational noise exposure was a risk factor of hypertension.

Methods: A questionnaire was designed to collect information from 738 study participants, all of whom were employees from the Datun Xuzhou Coal Company.

Analysis of plasma microRNA expression profiles in a Chinese population occupationally exposed to benzene and in a population with chronic benzene poisoning.

Background: Circulating microRNA (miRNA) has attractive interests as a non-invasive biomarker of physiological and pathological conditions. Our study aimed to investigate the potential effects of chronic benzene poisoning (CBP) and benzene exposure on miRNA expression, and identify CBP-related miRNAs.

Methods: In the discovery stage, we used a microarray assay to detect the miRNA expression profiles among pooled plasma samples from ten CBP patients, ten healthy benzene-exposed individuals and ten non-benzene exposed individuals.

Proteomic profiling change during the early development of silicosis disease.

Background: Silicosis is one of several severe occupational diseases for which effective diagnostic tools during early development are currently unavailable. In this study we focused on proteomic profiling during the early stages of silicosis to investigate the pathophysiology and identify the proteins involved.

Methods: Two-dimensional (2D) gel electrophoresis and MALDI-TOF-MS were used to assess the proteomic differences between healthy individuals (HI), dust-exposed workers without silicosis (DEW) and silicosis patients (SP).

Complete Genome Sequence of the Macrolide-Resistant Mycoplasma pneumoniae Strain C267 in China.

Macrolide-resistantMycoplasma pneumoniaestrains can cause severeM. pneumoniaepneumonia in children. Here, we report the complete genome sequence of the macrolide-resistantM.

Whole-Genome Sequencing of Macrolide-Resistant Mycoplasma pneumoniae Strain S355, Isolated in China.

Macrolide-resistant Mycoplasma pneumoniae plays an important role in refractory M. pneumoniae pneumonia. Here, we present the whole-genome sequencing of the macrolide-resistant M.

[Occupational noise-induced deafuess diaqnosis analysis in Jiangsu from 2006 to 2009].

Objective: In order to provide the evidence for taking the measures to prevent noise hazards, the condition sand influence factors of occupational noise deafness diagnosis in Jiangsu province from 2006 to 2013 were analyzed.

Methods: According to the 13 administrating personnel report of noise deafness diagnosed occupational disease prevention and control institutions in Jiangsu province from 2006 to 2013, the diagnosis of occupational noise deafness were retrospectively analyzed.

Results: By the end of December 31 2013, 297 cases of noise deafness were diagnosis occupational disease within this province.

[Distribution of copper and zinc level in urine of general population in eight provinces of China].

Objective: To describe the distribution of zinc (Zn) and copper (Cu) in urine samples of generalpopulation in eight provinces of China, to analyze their characteristics of distribution between different region, gender and age-cohorts, and to provide the baseline of themetabolites in the general population.

Methods: From 2009 to 2010, 18 120 subjects from the general population aged from 6 to 60 years old were recruited from 24 areas among 8 provinces of China mainland by random sampling. The environmental and physical condition characteristics were collected from questionnaires, and urine samples were collected at the mean time.