[Recovery of Phosphorus in the Form of Struvite from the Anaerobic Fermentation Supernatant of Excess Sludge].

To recover nitrogen and phosphorus from the supernatant of anaerobic fermentation of excess sludge in the form of struvite (MAP, MgNHPO·6HO), the optimum conditions of nitrogen and phosphorus dissolution and the effects of different reaction conditions on nitrogen and phosphorus recovery were studied. The results showed that the concentration of ammonia nitrogen and orthophosphate that dissolved in the fermentation liquid peaked on the fifth day of the experiment at pH 10.5 and 35℃.

Long intergenic noncoding RNA 01296 aggravates gastric cancer cells progress through miR-122/MMP-9.

Long non-coding RNAs (lncRNAs) play important roles on tumor development and progression in gastric cancer (GC). However, the biological function and regulatory mechanisms of LINC01296 in GC still remain unknown. The objective of this study is to investigate the clinical significance and pathological roles of LINC01296 in GC.

Integration of genomic and proteomic data to identify candidate genes in HT-29 cells after incubation with Bifidobacterium bifidum ATCC 29521.

As the predominant group inhabiting the human gastrointestinal tract, bifidobacteria play a vital role in human nutrition, therapeutics, and health by shaping and maintaining the gut ecosystem, reducing blood cholesterol, and promoting the supply of nutrients. The interaction between bacterial cells and human intestinal epithelial cell lines has been studied for decades in an attempt to understand the mechanisms of action. These studies, however, have been limited by lack of genomic and proteomic database to aid in achieving comprehensive understanding of these mechanisms at molecular levels.

Efficacy of oral Bifidobacterium bifidum ATCC 29521 on microflora and antioxidant in mice.

This study aimed to examine whether Bifidobacterium bifidum ATCC 29521, a species of colonic microflora in humans, is involved in the intestinal tract of mice. This study was also conducted to determine the antioxidant activity of this species by evaluating different microbial populations and reactive oxygen species isolated from feces and intestinal contents for 28 days of oral administration. Microbial diversities were assessed through bacterial culture techniques, PCR-DGGE, and real-time PCR.

Oral administration of Bifidobacterim bifidum for modulating microflora, acid and bile resistance, and physiological indices in mice.

Bifidobacteria are generally acknowledged as major gut microflora used as probiotics, which promote human health. In this study, the effects of the administration of Bifidobacterim bifidum on modulating gastrointestinal (GI) tract microflora, acid and bile resistance, and physiological indices in BALB/c mice were investigated. Results showed that B.

[Emission characteristics of PM2.5 from blast furnace iron making].

Electrical low pressure impactor (ELPI) was used to online analyze the PM2.5 particle size and mass concentration distribution in the trapping field and ore tank of blast furnace iron-making plant. Results showed that the grain number concentration of PM2.

Immunochromatographic strip for rapid detection of Cronobacter in powdered infant formula in combination with silica-coated magnetic nanoparticles separation and 16S rRNA probe.

Here we developed a sensitive, specific, and rapid immunochromatographic strip test for the detection of Cronobacter. Silica-coated magnetic nanoparticles were used to separate nucleic acid from Cronobacter lysate and eliminate the interference of food matrices successfully. A couple of 5'-end labeled probes, which was complementary to the 16S ribosomal DNA of Cronobacter, was used to hybridize with the nucleic acid.

[Interim report of prospective clinical study of two different digestive tract reconstruction after total gastrectomy].

Objective: To compare post-operative long-term complications and quality of life of two digestive reconstruction procedures after total gastrectomy.

Methods: A total of 109 gastric cancer patients in Tianjin Medical University Cancer Hospital from March 2012 to February 2013 were prospectively enrolled and randomly divided into functional jejunal interposition (FJI) group (52 cases) and Roux-en-Y (R-Y) group (57 cases). The post-operative complications, nutritional status, and the quality of life were compared between two groups.

Characteristics and prognosis of gastric cancer in patients aged ≥ 70 years.

Aim: To elucidate the prognostic value of age for gastric cancer and identify the optimal treatment for elderly gastric cancer patients.

Methods: We enrolled 920 patients with gastric cancer who underwent gastrectomy between January 2003 and December 2007 in our center. Patients were categorized into three groups: younger group (age < 50 years), middle-aged group (50-69 years), and elderly group (≥ 70 years).

Impact of intraoperative blood loss on survival after curative resection for gastric cancer.

Aim: To elucidate the potential impact of intraoperative blood loss (IBL) on long-term survival of gastric cancer patients after curative surgery.

Methods: A total of 845 stage I-III gastric cancer patients who underwent curative gastrectomy between January 2003 and December 2007 in our center were enrolled in this study. Patients were divided into 3 groups according to the amount of IBL: group 1 (< 200 mL), group 2 (200-400 mL) and group 3 (> 400 mL).

[Comparative study on four different reconstruction procedures after total gastrectomy].

Objective: To investigate the optimal reconstruction technique after total gastrectomy.

Methods: A total of 159 patients with gastric cancer undergoing total gastrectomy in Tianjin Cancer Hospital between January 2005 and December 2007 were divided into 4 groups according to the reconstruction technique: group A(functional jejunal interposition with a pouch, n=46), group B(modified Braun type II(, n=38), group C (P pouch with Roux-en-Y esophagojejunostomy, n=25), group D(Roux-en-Y esophagojejunostomy, n=50). Quality of life(QOL), nutritional status 1 year after surgery, and perioperative complications were analyzed.

[Risk factors of early recurrence in patients with gastric cancer after curative resection].

Objective: To analyze the clinical and pathologic influencing factors of early recurrence in patents with gastric cancer after radical gastrectomy.

Methods: Clinicopathological data of 141 patients with recurrence after curative gastrectomy for gastric cancer from January 2001 to December 2004 were analyzed retrospectively. Risk factors correlated with tumor early recurrence and survival difference between early recurrence group (< 1 year, 82 cases) and control group (1 year after, 59 cases) were assessed.

Accessory extrahepatic arteries: Blood supply of a human liver by three arteries A case report with brief literature review.

The coexistence of three hepatic arteries - accessory left hepatic artery (aLHA), proper hepatic artery (pHA), and accessory right hepatic artery (aRHA) - was demonstrated during a routine dissection of an 85-year-old male cadaver. The aLHA arose from the left gastric artery and ran in the hepatogastric ligament, whereas the aRHA took its origin from the superior mesenteric artery and ran in the hepatoduodenal ligament. The anatomy of the origins and the course of the arteries in the liver are described.

[Comparison of the prognostic value of UICC and JGCA lymph node staging criteria for gastric cancer].

Objective: To compare the correlation of prognosis with UICC or JGCA lymph node staging criteria for gastric cancer and evaluate the value of application of those two TNM staging systems in prognosis prediction.

Methods: From January 1996 to December 2005, 395 gastric cancer patients who underwent D2 or D2 plus radical gastrectomy with > or = 15 lymph nodes removed were enrolled into this study. The data were analyzed by both UICC and JGCA lymph node staging criteria, respectively.

Multiphoton-mediated corneal flap generation using the 80 MHz nanojoule femtosecond near-infrared laser.

Purpose: To evaluate whether corneal flaps can be generated by the 80 MHz near-infrared, intense nanojoule femtosecond laser based on multiphoton absorption.

Methods: A solid-state Ti:Sapphire femtosecond laser system was integrated in an inverted JenLab Femt-O-Cut laser scanning microscope. A diffraction-limited 40x objective was used to induce multiphoton ionization and plasma production.

[Expression change of apoptosis-associated genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer-transplanted nude mice].

Objective: To investigate the expression change of apoptosis-associated genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy and radiotherapy.

Methods: Human colon cancer cell line HT29 was transplanted into the hind limbs of nude mice. Under the laboratory-simulated condition of hyperthermia(43 degree centigrade, 60 min), actual radiation doses and MMC doses were calculated in reference to the clinical practice.

High-resolution two-photon excitation microscopy of ocular tissues in porcine eye.

Background And Objective: Two-photon excitation laser scanning microscopy (TPM), based on nonlinear optical (NLO) response under high irradiance, is currently being extensively employed for diagnostic purposes in biomedical fields and becomes more and more an interesting imaging technique in the intact bulk tissue examination. In this study, this nonlinear-excitation imaging technique including two-photon-mediated autofluorescence (2PF) and second harmonic generation (SHG) was employed to investigate the microstructures in the whole-mount scleral, retinal, and corneal tissues of porcine eyes with intracellular spatial resolution and high signal-to-noise ratio.

Materials And Methods: Image acquisition was based on the intense 80 MHz femtosecond (fs) near-infrared (NIR) laser pulses, emitted from a mode-locked solid-state titanium:sapphire system.

[Management of nonfunctioning islet cell tumors of the pancreas].

Objective: To analyze the clinical and pathological features in order to investigate appropriate way of diagnosis and treatment for non-functional islet cell tumors of the pancreas (NFICT).

Methods: The data and experience of surgically treated 43 patients with pathologically confirmed NFICT over the last 30 years were retrospectively reviewed. The survival rate was estimated using Kaplan-Meier method and the potential risk factors affecting survival were compared with Log rank test.

Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice.

Aim: To investigate the change in expression of p53, Bcl-2, and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy and thermochemoradiotherapy.

Methods: Human colon cancer cell line (HT29) was transplanted into the hind limbs of nude mice. Under laboratory simulated conditions of hyperthermia (43 centigrade, 60 min), the actual radiation doses and doses of mitomycin C (MMC) were calculated in reference to the clinical radiotherapy for human rectal cancer and chemotherapy prescription for colon cancer.

Multiphoton microscopy for monitoring intratissue femtosecond laser surgery effects.

Background And Objective: Multiphoton microscopy/tomography has been used as a novel diagnostic method for corneal imaging with subcellular resolution. Here, we used this technique to monitor femtosecond laser intratissue surgery effects.

Materials And Methods: Multiphoton microscopy/tomography on rabbits based on intense 90 MHz femtosecond Ti: sapphire laser was realized at intensities of MW-GW/cm(2), whereas the surgical procedures were performed with the same system at a higher light intensity on the order of TW/cm(2).

[Analysis of the risk factors of upper urinary tract dilatation due to spinal cord injury].

Objective: To evaluate the urodynamic risk factors of upper urinary tract dilatation (UUTD) secondary to spinal cord injury (SCI).

Methods: Ninety-six SCI patients of Tangshan earthquake were divided into 2 groups by ultrasonography: 16 SCI patients (group A) with UUTD and 80 SCI patients (group B) without UUTD received urodynamic test. Responses were evaluated using single and multiple analysis after examination.

In-vivo intratissue ablation by nanojoule near-infrared femtosecond laser pulses.

Non-invasive intratissue ablation was performed in the cornea of living rabbits by using 80 MHz near-infrared intense nanojoule femtosecond laser pulses. The intratissue surgical effect was induced by multiphoton absorption at a wavelength of 800 nm and was ascertained by histological examination. Highly precise intratissue ablation was obtained with no detrimental effects to the overlying or underlying layers.

Corneal multiphoton microscopy and intratissue optical nanosurgery by nanojoule femtosecond near-infrared pulsed lasers.

Multiphoton microscopy including multiphoton autofluorescence imaging (MAI) and second-harmonic generation (SHG) is being used as a novel diagnostic tool to perform tissue nonlinear optical tomography with submicron resolution. The three-dimensional corneal ultrastructure of whole depth has been viewed without any staining or mechanical slicing. Compared with photodisruptive surgical effects occurring at TW/cm2 light intensity, multiphoton imaging can be induced at MW-GW/ cm2 photon intensity.

Intraocular multiphoton microscopy with subcellular spatial resolution by infrared femtosecond lasers.

We reported on the in situ nonlinear optical sectioning of the corneal and retinal tissues based on the multiphoton microscopy (MPM) with different excitation wavelengths of infrared femtosecond (fs) lasers. The multiphoton nonlinear processing including two-photon fluorescence (2PF) and second harmonic generation (SHG) was induced under condition of high light intensities on an order of MW-GW/cm2. The laser beams emitted from the solid-state Ti: sapphire systems were focused in a 0.

Prognostic factors of young patients with colon cancer after surgery.

Aim: To investigate the prognostic factors of 96 young patients with colon cancer within a cancer center by univariate and multivariate analysis.

Methods: A total of 723 patients with colon cancer were treated surgically during a period of 10 years. Ninety six of them were 40 years old or younger.