[Relationship Between the VNTR Loci Polymorphism and MALDI-TOF MS Protein Profile Diversity in Complex].

Objective: To study the relationship between the copy numbers of repetitive units at variable number tandem repeat (VNTR) loci of complex (MTBC) with its diversity of protein profiles.

Methods: The MTBC strains were subjected to genotyping using multiple locus variable number tandem repeat analysis (MLVA). Also, the principal component analysis (PCA) was performed for bacterial protein profiles of MTBC using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).

[Electrochemical fingerprint of traditional Chinese medicines based on BrO⁻₃-Ce⁴⁺-H⁺-malonic acid/tartaric acid chemical oscillating system].

A new composite organic oscillating reaction system based on BrO₃-Ce(SO₄)₂-H₂SO₄-malonic acid/tartaric acid was proposed in this paper. On the basis of the influence of the concentration of each component on the stability and characteristic parameters of the blank system, the electrochemical fingerprints of 30 kinds of traditional Chinese medicines (TCM) were obtained. The results showed that the electrochemical fingerprint can be used for the identification of TCMs, the distinguishment of different parts and the appraisal of genuineness, which is fast, sensitive and accurate.

Honatisine, a novel diterpenoid alkaloid, and six known alkaloids from Delphinium honanense and their cytotoxic activity.

A novel diterpene alkaloid named honatisine (1) has been isolated from the whole plants of Delphinium honanense, along with six known alkaloids, siwanine E (2), isoatisine (3), atisine (4), delcorinine (5), uraphine (6), and nordhagenine A (7). Their structures were deduced on the basis of their spectral data. All of them were evaluated by a SRB assay for their cytotoxicity, and compound 1 showed a significant cytotoxic activity (IC(50) =3.

[The influence of lysogenic phage on biofilm formation of Pseudomonas aeruginosa].

Objective: To investigate the effect of the lysogenic phage Ppa3094 on the biofilm formation of PA3094.

Methods: The modified plate culture method was used to established the biofilm model in vitro. The viable counts of bacteria in biofilm were detected by MTT method; The real-time RT-PCR was applied to measure the expression level of algC and algD during the biofilm formtion of PA3094 and PA3094-L.

[Analysis of class I integrons in metalloenzyme-producing Pseudomonas aeruginosa].

Objective: To investigate class I integrons and integrated gene cassettes in metalloenzyme-producing Pseudomonas aeruginosa.

Methods: A total of 68 isolated clinical strains of Pseudomonas aeruginosa were subjected to PCR analysis with primers specific for bla(IMP-1) and bla(VIM). The positive strains then underwent examination for class I integrons and integrated gene cassettes with PCR with primers specific to class I integrase ((IntI)1) and integrated gene cassettes, followed by sequence analysis for some of the positive strains.

[The cloning and expression of rhlR, a quorum sensing gene in Pseudomonas aeruginosa PAO1, and a study of its effect on bacterial clearance rate in mouse lung].

Objective: To inquire about the molecular characteristics of rhlR, a Quorum Sensing gene in Pseudomonas aeruginosa (P. aeruginosa) PAO1, and to explore the immunogenicity of RhlR protein in mouse.

Methods: The rhlR gene of PAO1 was amplified by PCR and cloned into pGEX4T-1 plasmid.