Publications by authors named "Bao-Xia Ma"
Article Synopsis
- Scientists are trying to improve gene editing in cells by using new techniques that help fix DNA more effectively.
- They developed smaller proteins (SlugCas9 and AsCas12a) that make it easier to use these techniques compared to bigger proteins that were hard to handle.
- Tests showed that these new methods worked better and achieved higher gene editing efficiency, making it easier for researchers to use gene editing in their studies.
Gene knock-in in mammalian cells usually uses homology-directed repair (HDR) mechanism to integrate exogenous DNA template into the target genome site. However, HDR efficiency is often low, and the co-localization of exogenous DNA template and target genome site is one of the key limiting factors. To improve the efficiency of HDR mediated by CRISPR/Cas9 system, our team and previous studies fused different adaptor proteins with SpCas9 protein and expressed them.
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- Researchers developed a new Donor Adapting System (DAS) to enhance the efficiency of homology-directed repair (HDR) in CRISPR gene editing by using a Gal4 DNA binding domain in conjunction with the Cas9 protein.
- The study showed that using a specific length of homologous arms (100-60 bp) in the donor DNA could improve HDR editing efficiency by 2-4 times in a cell line experiment.
- Further optimizations indicated that the choice of fusion components and aptamer designs significantly impact Cas9's performance and HDR success, leading to effective editing at specific genetic sites, which could aid in animal breeding and molecular design.
In situ synthesis feasibility of ZrB-SiC-ZrC composite coatings on ZrC ceramics by reactive plasma spraying (RPS) was investigated. To help to understand the phase evolution during plasma spraying process, reaction behavior in the ZrH-Si-BC system was explored carefully by differential scanning calorimetry. The results indicated that the phase transformation sequence in the ZrH-Si-BC system could be described as ZrH, ZrO, ZrC, ZrB, ZrSi, ZrSi, and SiC.
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