Publications by authors named "Bao-Quan Guan"
Article Synopsis
- Research is advancing fluorescent proteins, focusing on developing brighter and diverse colored monomeric forms for better optical marking.
- A new variant, GFPxm, initially had limited use due to its low-temperature fluorescence; however, 12 mutant versions were created to enhance this property, with 7 showing strong fluorescence at higher temperatures.
- Some of the mutants outperformed the widely used EGFP in fluorescence intensity and could be expressed in mammalian cell lines, with various excitation peaks and a notable red shift in fluorescence spectra observed among several mutants.
[Orange fluorescent protein--modification of green fluorescent protein GFPxm].
Sheng Wu Gong Cheng Xue Bao
January 2003
Recently, we have reported a new gfp gene isolated from Aequorea macrodactyla. The protein purified from expressed E. coli exhibited an excitation peak at 476 nm and an emission peak at 496 nm.
View Article and Find Full Text PDFAim: To improve the immunogenicity of receptor binding site of hepatitis B virus (HBV) on preS1 antigen using HBV core antigen as an immuno-carrier.
Methods: One to 6 tandem copies of HBV preS1 (21-47) fragment were inserted into HBcAg at the sites of aa 78 and 82, and expressed in E.coli.
[Expression and bioactivity analysis of the fusion protein GFP-V(L) of the neutralizing monoclonal antibody MA18/7 against HBV].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
July 2004
Aim: To express the fusion protein of enhanced green fluorescent protein (EGFP) with the light chain variable domain of the neutralizing monoclonal antibody MA18/7 (mAb) against hepatitis B virus in E.coli, and determine its bioactivity.
Methods: The EGFP gene was cloned into vector pTO-T7 to construct an expression vector.