A high-performance liquid chromatography (HPLC) method was developed to analyze key compounds in Shengjiang Powder (SJP), like emodin and curcumin, and assess their effects on experimental autoimmune encephalomyelitis (EAE) in mice.
A total of 72 SPF C57 BL/6 female mice were assigned to different groups, including a control, model, various SJP dose groups, and a positive control, to study the therapeutic impacts of SJP following EAE induction.
The study found that SJP improved clinical symptoms, reduced inflammation and demyelination in brain tissues, while also increasing the expression of important proteins and cytokines involved in the JAK2/STAT3 signaling pathway.