A reusable evanescent wave immunosensor for highly sensitive detection of bisphenol A in water samples.

This paper proposed a compact and portable planar waveguide evanescent wave immunosensor (EWI) for highly sensitive detection of BPA. The incident light is coupled into the planar waveguide chip via a beveled angle through undergoing total internal reflection, where the evanescent wave field forms and excites the binding fluorophore-tagged antibodies on the chip surface. Typical calibration curves obtained for BPA has detection limits of 0.

Automated online optical biosensing system for continuous real-time determination of microcystin-LR with high sensitivity and specificity: early warning for cyanotoxin risk in drinking water sources.

The accelerated eutrophication of surface water sources and climate change have led to an annual occurrence of cyanobacterial blooms in many drinking water resources. To minimize the health risks to the public, cyanotoxin detection methods that are rapid, sensitive, real time, and high frequency must be established. In this study, an innovative automated online optical biosensing system (AOBS) was developed for the rapid detection and early warning of microcystin-LR (MC-LR), one of the most toxic cyanotoxins and most frequently detected in environmental water.

[Synthesis of complete antigen of microcystin-LR and its antibody production].

Based on the analysis of coupling location, coupling regent, carrying protein and coupling process, completed antigen of Microcystin-LR (MC-LR) was synthesized. A free amidogen was introduced to microcystin-LR (MC-LR) using chemical modification (aminoethylation) of its 7th core amino acids, N-methyl-dehydroalanine. The intermediate product Aminoethyl-MC-LR (H2N-etMC-LR) was purified by Solid Phase Elute (SPE) and identified by Mass Spectrometry, then coupled with BSA through glutaraldehyde to be complete antigen.