Orthogonal test design for optimization of the extraction of flavonid from the Fructus Gardeniae.

Objective: It is imperative to provide some consistent experimental results for the extraction of flavonid from Fructus Gardeniae.

Methods: The key extraction parameters that influenced the yield of flavonid from Fructus Gardeniae were optimized by employing an orthogonal experiment [L(9)(3)(4)], including the ratio of buffer solution (Na(2)B(4)O(7)·10H(2)O) to raw material, concentration of Fructus Gardeniae in extracting solution, extraction time and pH of buffer solution. An UV/Vis detector was used to perform the qualitative and quantitative analyses of the extracted flavonid with the using of the standard sample.