Copy number variation of the HPGDS gene in the Ashidan yak and its associations with growth traits.

Copy number variation (CNV) is a structural variation at the submicroscopic level of the genome, which can affect gene-related phenotypes by changing genes dosage and transcript structure. Hematopoietic prostaglandin D synthase (HPGDS) is a member whose functions are closely related to weight gain and inflammatory diseases of the glutathione S-transferase (GSTs) family. In this study, the growth characteristics (body weight, withers height, body length, and chest girth) of 336 Ashidan yaks were monitored at four stages (6 months, 12 months, 18 months, and 30 months).

Block-Localized Excitation for Excimer Complex and Diabatic Coupling.

We describe a block-localized excitation (BLE) method to carry out constrained optimization of block-localized orbitals for constructing valence bond-like, diabatic excited configurations using multistate density functional theory (MSDFT). The method is an extension of the previous block-localized wave function method through a fragment-based ΔSCF approach to optimize excited determinants within a molecular complex. In BLE, both the number of electrons and the electronic spin of different fragments in a whole system can be constrained, whereas electrostatic, exchange, and polarization interactions among different blocks can be fully taken into account of.

Characterization of the complete mitochondrial genome of the Meiren yak ().

In this study, high-throughput Illumina sequencing was employed to assemble the complete mitochondrial genome of the Meiren yak (), a local yak breed from Gansu Province, China. The mitochondrial genome is 16,321 bp long with an A + T-biased nucleotide composition and harbors 13 protein-coding, 22 Trna, and 2 rRNA genes, and a noncoding control region. The mitogenomic organization and codon usage are highly similar to those of previously published congeneric mitochondrial genomes.

The complete mitochondrial genome of the hybrid of Jersey cattle (; ♂) × Gannan yak (; ♀).

In this study, we reconstructed the complete mitochondrial genome of the hybrid of Jersey cattle (; ♂) × Gannan yak (; ♀) from Illumina sequencing reads. The mitochondrial genome is 16,322 bp in length with an A + T-biased nucleotide composition, and encodes 13 protein-coding genes, 22 tRNAs, and 2 rRNAs along with a noncoding control region. In addition, its gene order is identical to those of previously published mitochondrial genomes of the genera and .

Characterization of the complete mitochondrial genome of the Tianjun yak ().

Tianjun yak () is a yak breed with strong adaptation to the high-elevation, cold and anoxic environments. Its complete mitochondrial genome is 16,323 bp long with an asymmetric base composition, and harbors the 37 typical mitochondrial genes and one noncoding control region. The PCGs are initiated with the typical ATA or ATG codons, and are terminated with TAA, TAG or the incomplete stop codon T--.

Characterisation of the complete mitochondrial genome of the Jinchuan Yak ().

Jinchuan yak was a newly discovered yak breed, not only possesses a large proportion of multi-ribs but also exhibits many good characteristics. However, there is limited information about its overall genetic structure. In this study, we assembled the mitochondrial genome for Jinchuan yak (), the results show that the mitochondrial genome is 16,324bp long with an A + T-biased base composition (61.

Generalization of Block-Localized Wave Function for Constrained Optimization of Excited Determinants.

The block-localized wave function method is useful to provide insights on chemical bonding and intermolecular interactions through energy decomposition analysis. The method relies on block localization of molecular orbitals (MOs) by constraining the orbitals to basis functions within given blocks. Here, a generalized block-localized orbital (GBLO) method is described to allow both physically localized and delocalized MOs to be constrained in orbital-block definitions.

[Nutritional risk screening and related factors of cancer patients in community of Shanghai, 2018-2019].

To study the risk of malnutrition and related factors of cancer patients in community of Shanghai. From October 2018 to January 2019, four communities, Pengpu New Village Street, Pengpu Town, Jiangning Road Street, and Caojiadu Street, from 14 communities in Jing 'an District, Shanghai City, were selected by using a random cluster sampling method based on the Shanghai Cancer Registration and reporting system. All cases of malignant tumors and benign tumors of the central nervous system were included.

Transcriptome Analysis Reveals the Potential Role of Long Non-coding RNAs in Mammary Gland of Yak During Lactation and Dry Period.

The mammary gland is a remarkably dynamic organ of milk synthesis and secretion, and it experiences drastic structural and metabolic changes during the transition from dry periods to lactation, which involves the expression and regulation of numerous genes and regulatory factors. Long non-coding RNA (lncRNA) has considered as a novel type of regulatory factors involved in a variety of biological processes. However, their role in the lactation cycle of yak is still poorly understood.

Research progress of ulinastatin in the treatment of liver diseases.

Ulinastatin (UTI) is a trypsin inhibitor observed in urine. UTI can treat some diseases by inhibiting the broad-spectrum hydrolysis activity of various enzymes and other pharmacological effects. UTI can widely treat pancreatitis, systemic multiple organ dysfunction syndrome, circulatory failure, and toxic shock clinically.

Impact of the Order of Legendre Polynomials in Random Regression Model on Genetic Evaluation for Milk Yield in Dairy Cattle Population.

The random regression test-day model has become the most commonly adopted model for routine genetic evaluations in dairy populations, which allows accurately accounting for genetic and environmental effects over lactation. The objective of this study was to explore appropriate random regression test-day models for genetic evaluation of milk yield in a Holstein population with a relatively small size, which is the common situation in regional or independent breeding companies to preform genetic evaluation. Data included 419,567 test-day records from 54,417 cows from the first lactation.

Association between lincRNA expression and overall survival for patients with triple-negative breast cancer.

Purpose: Long intergenic non-coding RNAs (lincRNAs) are increasingly recognized as important regulators for pathogenesis and/or prognosis of breast cancer, including triple-negative breast cancer (TNBC) subtype. However, few previous studies used RNA-sequencing (RNA-Seq) technology, and none included an independent replication.

Methods: To systematically evaluate the association between expression of lincRNAs and TNBC survival, we examined lincRNA expression profiles in TNBC tissues using RNA-Seq data for 200 TNBC patients from the Shanghai Breast Cancer Survival Study (SBCSS) and Southern Community Cohort Study (SCCS).

Reduced Sleep in the Week Prior to Diagnosis of COVID-19 is Associated with the Severity of COVID-19.

Background: The rapid outbreak of coronavirus disease 2019 (COVID-19) is a major health concern, in response to which widespread risk factor research is being carried out.

Objective: To discover how physical activity and lifestyle affect the epidemic as well as the disease severity and prognosis of COVID-19 patients.

Methods: This multicenter, retrospective cohort study included 203 adults infected with COVID-19 and 228 uninfected adults in three Chinese provinces, with 164 (80.

Secondary Structural Transformation of Bovine Lactoferricin Affects Its Antibacterial Activity.

Lactoferricin (Lfcin) is a potent antibacterial peptide derived from lactoferrin by pepsin hydrolysis. It was hypothesized that structural transformation of Lfcin could affect its antibacterial function through forming and breaking of intramolecular disulfide bond. To prove this hypothesis, bovine Lfcin (bLfcin) and its two derivatives, bLfcin with a disulfide bond (bLfcin DB) and bLfcin with a mutation C36G (bLfcin C36G), were synthesized, purified, and identified.

A Retrospective Analysis of the Trends in Acute Appendicitis During the COVID-19 Pandemic.

The corona virus disease of 2019 (COVID-19) imposed new public health constraints that deterred people from coming to the hospital. The outcome of patients who developed appendicitis during mandated COVID-19 quarantine has yet to be examined. The main objective was to establish whether there was an increased rate of perforated appendicitis seen during COVID-19 quarantine.

A peptide-based aggregation-induced emission bioprobe for selective detection and photodynamic killing of Gram-negative bacteria.

Herein, we report a new bioprobe with aggregation-induced emission (AIE) characteristics by conjugation of a far-red/near-infrared emissive AIE luminogen and two polymyxinB peptides. Due to the strong binding effect between polymyxin B and the lipopolysaccharide in the cell wall of Gram-negative bacteria, the bioprobe can selectively visualize Gram-negative bacteria and effectively kill them via photodynamic treatment.

Circ_0006528 Contributes to Paclitaxel Resistance of Breast Cancer Cells by Regulating miR-1299/CDK8 Axis.

Background: Circular RNAs (circRNAs) have been reported to be involved in regulating the development of breast cancer. Paclitaxel (PTX) can be used for the chemotherapy of breast cancer. The study aimed to explore the role and mechanism of circ_0006528 in PTX-resistant breast cancer progression.

Prognostic Assessment of COVID-19 in the Intensive Care Unit by Machine Learning Methods: Model Development and Validation.

Background: Patients with COVID-19 in the intensive care unit (ICU) have a high mortality rate, and methods to assess patients' prognosis early and administer precise treatment are of great significance.

Objective: The aim of this study was to use machine learning to construct a model for the analysis of risk factors and prediction of mortality among ICU patients with COVID-19.

Methods: In this study, 123 patients with COVID-19 in the ICU of Vulcan Hill Hospital were retrospectively selected from the database, and the data were randomly divided into a training data set (n=98) and test data set (n=25) with a 4:1 ratio.

Accuracies of Genomic Prediction for Growth Traits at Weaning and Yearling Ages in Yak.

Genomic selection is a promising breeding strategy that has been used in considerable numbers of breeding projects due to its highly accurate results. Yak are rare mammals that are remarkable because of their ability to survive in the extreme and harsh conditions predominantly at the so-called "roof of the world"-the Qinghai-Tibetan Plateau. In the current study, we conducted an exploration of the feasibility of genomic evaluation and compared the predictive accuracy of early growth traits with five different approaches.

Genome-wide detection and sequence conservation analysis of long non-coding RNA during hair follicle cycle of yak.

Background: Long non-coding RNA (lncRNA) as an important regulator has been demonstrated playing an indispensable role in the biological process of hair follicles (HFs) growth. However, their function and expression profile in the HFs cycle of yak are yet unknown. Only a few functional lncRNAs have been identified, partly due to the low sequence conservation and lack of identified conserved properties in lncRNAs.

[Application of micellar electrokinetic capillary chromatography for identification, quantitative detection and unfolding analysis of interleukin-12].

Objective: To establish a micellar electrokinetic capillary chromatography-based method for identification and quantitative detection of interleukin-12 (IL-12) and analysis of its unfolding process.

Methods: An uncoated fused-silica capillary (inner diameter 50 μm) with a total length of 48.5 cm (40 cm to the detector) was used for the experiment.

Convalescent plasma therapy in critically ill coronavirus disease 2019 patients with persistently positive nucleic acid test, case series report.

Introduction: Globally, the coronavirus disease 2019 (COVID-19) is still spreading rapidly. At present, there are no specifically approved therapeutic agents or vaccines for its treatment. Previous studies have shown that the convalescent plasma therapy (CPT) is effective in patients with COVID-19.

Metagenomic and C tracing evidence for autotrophic microbial CO fixation in paddy soils.

Autotrophic carbon dioxide (CO ) fixation by microbes is ubiquitous in the environment and potentially contributes to the soil organic carbon (SOC) pool. However, the multiple autotrophic pathways of microbial carbon assimilation and fixation in paddy soils remain poorly characterized. In this study, we combine metagenomic analysis with C-labelling to investigate all known autotrophic pathways and CO assimilation mechanisms in five typical paddy soils from southern China.

Detection of InDel and CNV of gene and their associations with bovine growth traits.

Sperm-associated antigen 17 () gene encodes a central pair protein, which is involved in flagellar motility, male fertility and skeletal growth in ruminants. The insertions/deletions (indels) and copy number variations (CNVs) influence phenotypic traits by altering the sequences and copy numbers of functional genes, respectively. This study identified a novel 8-bp indel of gene in 1520 individuals from eight different cattle breeds, as well as a novel CNV region in 355 animals.