Characteristics of Soluble PD-L1 and PD-1 Expression and Their Correlations With Immune Status and Prognosis in Advanced Lung Cancer.

Purpose: Our study aims to evaluate the characteristics of serum soluble PD-1 (sPD-1) and soluble PD-L1 (sPD-L1) levels and their correlations with immune status and prognosis in advanced lung cancer patients.

Methods: Patients diagnosed with advanced lung cancer based on histology or cytology in Peking University People's Hospital from July 2020 to November 2021 were enrolled. Clinicopathological data were recorded and analyzed.

Analysis and Validation of a Diagnostic Nomogram for Predicting the Risk of Acute Respiratory Failure for Non-HIV Related Pneumocystis Jirovecii Pneumonia Patients.

Objective: Pneumocystis Pneumonia (PCP), primarily affecting individuals with weakened immune systems, is a severe respiratory infection caused by pneumocystis jirovecii and can lead to acute respiratory failure (ARF). In this article, we explore the risk factors of ARF and propose a prognostic model of ARF for PCP patients.

Methods: In this multi-center, retrospective study in 6 secondary or tertiary academic hospitals in China, 120 PCP patients were screened from the Dryad database for the development of a predictive model.

Reversible cold-induced lens opacity in a hibernator reveals a molecular target for treating cataracts.

Maintaining protein homeostasis (proteostasis) requires precise control of protein folding and degradation. Failure to properly respond to stresses disrupts proteostasis, which is a hallmark of many diseases, including cataracts. Hibernators are natural cold-stress adaptors; however, little is known about how they keep a balanced proteome under conditions of drastic temperature shift.

Current chemotherapy strategies for adults with IDH-wildtype glioblastoma.

Introduction: Glioblastoma, despite advancements in molecular evolution, remains incurable and has low survival rates. Currently, two of the most commonly used chemotherapy regimens are temozolomide and CCNU. This review aims to provide a comprehensive analysis of the current status of chemotherapy strategies for GBM.

Integrating Pt nanoparticles with 3D Cu Se/GO nanostructure to achieve nir-enhanced peroxidizing Nano-enzymes for dynamic monitoring the level of HO during the inflammation.

The treatment of wound inflammation is intricately linked to the concentration of reactive oxygen species (ROS) in the wound microenvironment. Among these ROS, HO serves as a critical signaling molecule and second messenger, necessitating the urgent need for its rapid real-time quantitative detection, as well as effective clearance, in the pursuit of effective wound inflammation treatment. Here, we exploited a sophisticated 3D Cu Se/GO nanostructure-based nanonzymatic HO electrochemical sensor, which is further decorated with evenly distributed Pt nanoparticles (Pt NPs) through electrodeposition.

UBE2D3 regulated by WTAP-mediated m6A modification inhibits temozolomide chemosensitivity in glioblastoma.

To explore how the ubiquitin-conjugating enzyme E2D3 (UBE2D3) influences temozolomide (TMZ) resistance in glioblastoma (GBM), and to clarify the association between UBE2D3 and WTAP. The UBE2D3 protein expression in GBM tissues were detected using immunohistochemistry (IHC) through tissue microarrays. The potential pathways of UBE2D3 in TCGA-GBM were predicted via Gene Set Enrichment Analysis (GSEA).

Lens autophagy protein ATG16L1: a potential target for cataract treatment.

Cataract is the leading cause of blindness and low vision worldwide, yet its pathological mechanism is not fully understood. Although macroautophagy/autophagy is recognized as essential for lens homeostasis and has shown potential in alleviating cataracts, its precise mechanism remains unclear. Uncovering the molecular details of autophagy in the lens could provide targeted therapeutic interventions alongside surgery.

Adsorption of Co and Cr in Industrial Wastewater by Magnesium Silicate Nanomaterials.

In this paper, two flower-like magnesium silicate nanomaterials were prepared. These and another two commercial magnesium silicate materials were characterized using a scanning electron microscope, the N adsorption-desorption method, and other methods. The structure-activity relationship between the adsorption performance of these four magnesium silicate materials and their specific surface area, pore size distribution, and pore structure was compared.

Association between weekend catch-up sleep and depression of the United States population from 2017 to 2018: A cross-sectional study.

Insufficient sleep on weekdays has become a societal norm, and studies have shown that sleep deprivation increases the risk of depression. Although individuals often resort to weekend catch-up sleep (CUS) as a compensatory measure, the present evidence supporting its efficacy in mitigating the risk of depression is limited. This article attempts to explore the relationship between CUS and depression.

Paclitaxel liposome (Lipusu) based chemotherapy combined with immunotherapy for advanced non-small cell lung cancer: a multicenter, retrospective real-world study.

Background: Paclitaxel liposome (Lipusu) is known to be effective in non-small cell lung cancer (NSCLC) as first-line treatment. This study aimed to evaluate the effectiveness and safety of paclitaxel liposome based chemotherapy plus PD-1/PD-L1 inhibitor in patients with advanced NSCLC.

Methods: In this multicenter, retrospective, real-world study, patients with advanced NSCLC who were administered paclitaxel liposome based chemotherapy plus PD-1/PD-L1 inhibitor in three centers (Peking University People's Hospital as the lead center) in China between 2016 and 2022 were included.

N6-methyladenosine-induced miR-182-5p promotes multiple myeloma tumorigenesis by regulating CAMK2N1.

Methyltransferase like 3 (METTL3) has been reported to promote tumorigenesis of multiple myeloma (MM), however, the molecular mechanism still needs further research. The N6-methyladenosine (m6A) level in tissues or cells was measured by m6A kit and dot blot assay. The mRNA and protein expression were detected by quantitative real-time PCR (RT-qPCR) and Western blot, respectively.

Associations Between Adult Attachment, Maternal Emotion Socialization and Child Behavior.

Background: Attachment theories suggest that the adult attachment styles of mothers may influence behavior problems among their children; however, empirical studies examining the direct role of maternal adult attachment styles and the mechanisms through emotion-related parenting are scarce.

Objective: This study examined the influence of maternal adult attachment styles and emotion-related parenting on child internalizing and externalizing problems.

Methods: The participants were 409 mothers with children aged 4-5 years.

Initial Treatment of IDH-Wildtype Glioblastoma in Adults Older Than 70 Years.

The incidence of glioblastoma, the most common malignant primary brain tumour in adults, increases after the age of 40 and peaks in adults aged 75-84 years. Initial management involves maximising surgical resection while preserving neurologic function. mutations and MGMT promoter methylation should be checked in tumour samples.

Discussion on Improvement of the Incidence of Complications After the Application of Self-Management and Continuous Nursing in Children with Primary Nephrotic Syndrome.

Objective: To analyze the effects of self-management and continuous nursing on improving the incidence of complications in children with primary nephrotic syndrome.

Methods: A retrospective analysis of 80 cases of children with primary nephrotic syndrome treated in our hospital from January 2019 to October 2022 was conducted. The patients were divided into a control group and an observation group, with 40 cases in each group.

Urokinase Salvage Therapy for Recurrent Chronic Subdural Hematoma Caused by Dual Antiplatelet Therapy.

Chronic subdural hematoma (cSDH) is one of the most common neurosurgical conditions among older adults. Here, we describe a case of recurrent cSDH resulting from seven days of dual antiplatelet therapy. A 76-year-old woman reported a headache resembling a chronic tension-type headache for the last 10 days.

T2T-YAO: A Telomere-to-telomere Assembled Diploid Reference Genome for Han Chinese.

Since its initial release in 2001, the human reference genome has undergone continuous improvement in quality, and the recently released telomere-to-telomere (T2T) version - T2T-CHM13 - reaches its highest level of continuity and accuracy after 20 years of effort by working on a simplified, nearly homozygous genome of a hydatidiform mole cell line. Here, to provide an authentic complete diploid human genome reference for the Han Chinese, the largest population in the world, we assembled the genome of a male Han Chinese individual, T2T-YAO, which includes T2T assemblies of all the 22 + X + M and 22 + Y chromosomes in both haploids. The quality of T2T-YAO is much better than those of all currently available diploid assemblies, and its haploid version, T2T-YAO-hp, generated by selecting the better assembly for each autosome, reaches the top quality of fewer than one error per 29.

HOXA1 participates in VSMC-to-macrophage-like cell transformation via regulation of NF-κB p65 and KLF4: a potential mechanism of atherosclerosis pathogenesis.

Background: Macrophage-like transformation of vascular smooth muscle cells (VSMCs) is a risk factor of atherosclerosis (AS) progression. Transcription factor homeobox A1 (HOXA1) plays functional roles in differentiation and development. This study aims to explore the role of HOXA1 in VSMC transformation, thereby providing evidence for the potential mechanism of AS pathogenesis.

Modified approaches to prepare nano-magnesium silicates with hierarchical pore structure and their performance towards adsorption of Cd.

A series of flower-like magnesium silicate samples with hierarchical pore were prepared by the solvothermal method under template-free conditions using sodium dodecyl sulfate as the modifier and ethanol-water as the solvent. These samples were characterized by various methods and were evaluated for the adsorption of heavy metal Cd. The results showed that the adding modifier did not change the crystal structure of the magnesium silicate samples.

Glycolysis Aids in Human Lens Epithelial Cells' Adaptation to Hypoxia.

Hypoxic environments are known to trigger pathological damage in multiple cellular subtypes. Interestingly, the lens is a naturally hypoxic tissue, with glycolysis serving as its main source of energy. Hypoxia is essential for maintaining the long-term transparency of the lens in addition to avoiding nuclear cataracts.

Znf469 Plays a Critical Role in Regulating Synthesis of ECM: A Zebrafish Model of Brittle Cornea Syndrome.

Purpose: Brittle cornea syndrome (BCS) is a rare autosomal recessive disorder characterized by extreme thinning and fragility of the cornea, and mutations in ZNF469 cause BCS-1. We aimed to establish a znf469 mutant zebrafish line to explore its roles and possible pathogenic mechanism in cornea development and disorder.

Methods: znf4694del/4del mutant zebrafish was generated using the CRISPR/Cas9 technology.

[Enhanced mechanical properties and cytocompatibility of GelMA hydrogels by incorporating graphene oxide].

Purpose: To construct graphene oxide/methacrylated gelatin(GO/GelMA) hydrogel and to investigate its mechanical property and biocompatibility.

Methods: 50, 100, 200 μg/mL GO was added to GelMA and mixed thoroughly. The micromorphology of hydrogels was observed under scanning electron microscope(SEM).

Caffeine is negatively associated with depression in patients aged 20 and older.

Introduction: Previous studies have observed the association between caffeine intake and depression, but few have considered the potential threshold effect of this issue. Therefore, the study aimed to examine the association between caffeine consumption and depression in patients aged 20 years or older using curve fitting analysis.

Methods: The population was 3,263 patients from the 2017 to 2018 National Health and Nutrition Examination Survey (NHANES) with reliable answers to questions of caffeine intake and depression.