Detection of 4 quinolone antibiotics by chemiluminescence based on a novel Nor-Biotin bifunctional ligand and SA-ALP technology.

A simple and effective direct competitive chemiluminescence immunoassay for the detection of 4 kinds of quinolone antibiotics in milk was established using Nor-Biotin (biotin-modified norfloxacin [NOR]) bifunctional ligand and alkaline phosphatase-conjugated streptavidin signal amplification technology. The polyclonal antibody was obtained after the immunization of New Zealand White rabbits using norfloxacin-derived antigen. "Click chemistry" was used for the rapid and facile synthesis of the Nor-Biotin bifunctional ligand.

State-of-the-art progress of switch fluorescence biosensors based on metal-organic frameworks and nucleic acids.

Metal-organic frameworks (MOFs) have captured substantial attention of an increasing number of scientists working in sensing analysis fields, due to their large surface area, high porosity, and tunable structure. Recently, MOFs as attractive fluorescence quenchers have been extensively investigated. Given their high quenching efficiency toward the fluorescence intensity of dyes-labeled specific biological recognition molecules, such as nucleic acids, MOFs have been widely developed to switch fluorescence biosensors with low background fluorescence signal.

Development and perspectives of rapid detection technology in food and environment.

Food safety become a hot issue currently with globalization of food trade and food supply chains. Chemical pollution, microbial contamination and adulteration in food have attracted more attention worldwide. Contamination with antibiotics, estrogens and heavy metals in water environment and soil environment have also turn into an enormous threat to food safety.

A low-field nuclear magnetic resonance DNA-hydrogel nanoprobe for bisphenol A determination in drinking water.

A low-field nuclear magnetic resonance (LF-NMR) DNA-hydrogel (LNDH) nanoprobe was designed for bisphenol A (BPA) determination. It consists of FeO superparamagnetic iron oxide nanoparticles (SPIONs) and a DNA-hydrogel technology. FeO SPIONs were encapsulated in the DNA-hydrogel to form an aggregated state.

[High sensitive ELISA for detection of atrazion].

Objectives: To set up ELISA for detection of atrazine with high precision.

Methods: The reaction condition of indirect-ELISA was optimized, including atrazine-ovalbumin(AT-OVA) concentration and primary antibody concentration, organic solvent, goat anti-rat immunoglobin G-horseradish peroxidase(IgG-HRP) concentration. The actual samples were detected by the ELISA method established in our laboratory.

Distribution Characteristics of Spermophilus dauricus in Manchuria City in China in 2015 through '3S' Technology.

Plague is a virulent infectious disease in China. In this study, '3S' technology was used to perform spatial autocorrelation analysis and spatial interpolation analysis for Spermophilus dauricus (S. Dauricus, a species of ground squirrel) captured in Manchuria City in 2015.

[Purification of monoclonal antibody to clenbuterol and its biology identity].

Objective: To identify the self-preparation monoclonal antibody which target to clenbuterol, and set up the standard curve to clenbuterol (CL) detection.

Methods: The affinity constants and activity of the monoclonal antibody which target to CL were determined by ELISA. ELISA was also used to confirm whether the monoclonal antibody had any across-reaction with BSA and CL analogues.

Molecularly imprinted photonic polymer as an optical sensor to detect chloramphenicol.

An inverse opal photonic crystal sensor that could specifically detect chloramphenicol (CAP) in a label-free way was introduced in the current research. A colloidal crystal template was first prepared from monodisperse SiO(2) nanospheres. Precursors with different compositions were infused into the void spaces of the respective templates and aggregated.

[Preparation of clenbuterol monoclonal antibody with subtractive immunization method].

Aim: To obtain Clenbuterol monoclonal antibodies.

Methods: Clenbuterol complete antigen was prepared with diazotization method. BALB/c mice was immunized with subtractive immunization, Clenbuterol monoclonal antibody was prepared with rule hybridoma technique.

[Identification of exotoxin-specific motifs/domains in bacterial exotoxin sequences and corresponding gene ontology analysis].

Objective: To identify the exotoxin-specific motifs/domains in bacterial exotoxin sequences, and to expand understanding of bacterial exotoxins pathogenic mechanisms.

Methods: We constructed a non-pathogenic bacterial proteins database and collected 89 bacterial exotoxin sequences from Virulence factor database (VFDB), then we analyzed these protein sequences by motif/domain search using InterProScan (www.ebi.

A sensitive immunoassay based on direct hapten coated format and biotin-streptavidin system for the detection of chloramphenicol.

A novel enzyme liked immunosorbent assay (ELISA) was developed for the detection of chloramphenicol (CAP). In this assay, the small molecular hapten (Hap) was directly coated on the surface of microtiter plates and biotin-streptavidin system (BSAS) was employed to improve the sensitivity of immunoassay (BSAS-direct Hap coated ELISA). The surface of microtiter plates was treated with glutaraldehyde (GA) polymer network to introduce aldehyde group, which was used to cross-link with amino group of CAP.

A fluoroimmunoassay based on quantum dot-streptavidin conjugate for the detection of chlorpyrifos.

A rapid and sensitive competitive fluorescence-linked immunosorbent assay (cFLISA) based on quantum dot-streptavidin conjugate (QDs-SA) was developed for the detection of chlorpyrifos in drinking water. The QDs-SA conjugate, which consists of 3-mercaptopropyl acid-stabilized CdTe nanoparticle QDs and streptavidin (SA) made through the active ester method, was employed to improve the sensitivity of QDs-SA-cFLISA. The 50% inhibition concentration (IC50) and the limit of detection (LOD) were 28.

[Preparation and activity analysis of RGD-mSAK (K130T, K135R)].

In order to construct RGD-mSAK mutant with reduced immunogenicity, and identify its biological activity after purification, mSAK gene fragment was amplified by over-lapping extension PCR. Then the gene was inserted into the prokaryotic expression vector pBV220 with P(R)P(L) promoters after confirmed by DNA sequencing; the expression plasmid pBV220-RGD-mSAK was constructed, and then was transformed into E. coli.

[Preparation and the anti-tumor activity of mutant Staphylococcal enterotoxin B].

Aim: To prepare mutant Staphylococcal enterotoxin B(SEB) and observe its anti-tumor activity.

Methods: The expressed mutant SEB-K172E in inclusion body was denatured and renatured, and then isolated and purified. The anti-tumor activity of the mutant SEB-K172E was compared with wild-type SEB.