Effect of temperature on skeletal muscle energy turnover during dynamic knee-extensor exercise in humans.

The present study examined the effect of elevated temperature on muscle energy turnover during dynamic exercise. Nine male subjects performed 10 min of dynamic knee-extensor exercise at an intensity of 43 W (SD 10) and a frequency of 60 contractions per minute. Exercise was performed under normal (C) and elevated muscle temperature (HT) through passive heating.

Fatigue in soccer: a brief review.

This review describes when fatigue may develop during soccer games and the potential physiological mechanisms that cause fatigue in soccer. According to time-motion analyses and performance measures during match-play, fatigue or reduced performance seems to occur at three different stages in the game: (1) after short-term intense periods in both halves; (2) in the initial phase of the second half; and (3) towards the end of the game. Temporary fatigue after periods of intense exercise in the game does not appear to be linked directly to muscle glycogen concentration, lactate accumulation, acidity or the breakdown of creatine phosphate.

Physical demands during an elite female soccer game: importance of training status.

Purpose: To examine the activity profile and physical loading of elite female soccer players during match play and to study the relationship between training status and physical match performance.

Methods: Time-motion analysis and HR recordings were performed on 14 elite female soccer players during competitive matches. In addition, the players carried out a laboratory treadmill test and the Yo-Yo intermittent recovery test.

Dexamethasone up-regulates skeletal muscle maximal Na+,K+ pump activity by muscle group specific mechanisms in humans.

Dexamethasone, a widely clinically used glucocorticoid, increases human skeletal muscle Na+,K+ pump content, but the effects on maximal Na+,K+ pump activity and subunit specific mRNA are unknown. Ten healthy male subjects ingested dexamethasone for 5 days and the effects on Na+,K+ pump content, maximal activity and subunit specific mRNA level (alpha1, alpha2, beta1, beta2, beta3) in deltoid and vastus lateralis muscle were investigated. Before treatment, maximal Na+,K+ pump activity, as well as alpha1, alpha2, beta1 and beta2 mRNA levels were higher (P < 0.

Metabolic alkalosis reduces exercise-induced acidosis and potassium accumulation in human skeletal muscle interstitium.

Skeletal muscle releases potassium during activity. Interstitial potassium accumulation is important for muscle function and the development of fatigue resulting from exercise. In the present study we used sodium citrate ingestion as a tool to investigate the relationship between interstitial H+ concentration and K+ accumulation during exercise.

Contraction-induced increases in Na+-K+-ATPase mRNA levels in human skeletal muscle are not amplified by activation of additional muscle mass.

The present study tested the hypothesis that exercise with a large compared with a small active muscle mass results in a higher contraction-induced increase in Na(+)-K(+)-ATPase mRNA expression due to greater hormonal responses. Furthermore, the relative abundance of Na(+)-K(+)-ATPase subunit alpha(1), alpha(2), alpha(3), alpha(4), beta(1), beta(2), and beta(3) mRNA in human skeletal muscle was investigated. On two occasions, eight subjects performed one-legged knee extension exercise (L) or combined one-legged knee extension and bilateral arm cranking (AL) for 5.

Recruitment of fibre types and quadriceps muscle portions during repeated, intense knee-extensor exercise in humans.

To investigate recruitment of slow-twitch (ST) and fast-twitch (FT) muscle fibres, as well as the involvement of the various quadriceps femoris muscle portions during repeated, intense, one-legged knee-extensor exercise, 12 healthy male subjects performed two 3-min exercise bouts at approximately 110% maximum thigh O2 consumption (EX1 and EX2) separated by 6 min rest. Single-fibre metabolites were determined in successive muscle biopsies obtained from the vastus lateralis muscle (n = 6) and intra-muscular temperatures were continuously measured at six quadriceps muscle sites (n = 6). Creatine phosphate (CP) had decreased (P < 0.

Intense interval training enhances human skeletal muscle oxygen uptake in the initial phase of dynamic exercise at high but not at low intensities.

The present study tested the hypothesis that intense interval training enhances human skeletal muscle blood flow and oxygen uptake (VO2) at the onset of dynamic exercise. We also investigated whether possible training effects were dependent on exercise intensity. Six habitually active males carried out 7 weeks of intermittent-exercise one-legged knee-extensor training at an intensity corresponding to approximately 150% of peak thigh VO2 on three to five occasions per week.

Slow-twitch fiber glycogen depletion elevates moderate-exercise fast-twitch fiber activity and O2 uptake.

Purpose: We tested the hypotheses that previous glycogen depletion of slow-twitch (ST) fibers enhances recruitment of fast-twitch (FT) fibers, elevates energy requirement, and results in a slow component of VO2 during moderate-intensity dynamic exercise in humans.

Methods: Twelve healthy, male subjects cycled for 20 min at approximately 50% VO2max with normal glycogen stores (CON) and with exercise-induced glycogen depleted ST fibers (CHO-DEP). Pulmonary VO2 was measured continuously and single fiber, muscle homogenate, and blood metabolites were determined repeatedly during each trial.

Muscle temperature and sprint performance during soccer matches--beneficial effect of re-warm-up at half-time.

The relationship between quadriceps muscle temperature (T(m)) and sprint performance was evaluated during soccer matches in 25 competitive players. In one game, T(m) was determined frequently (n=9). In another game, eight players performed low-intensity activities at half-time (re-warm-up, (RW), whereas another eight players recovered passively (CON).

Potassium kinetics in human muscle interstitium during repeated intense exercise in relation to fatigue.

Accumulation of K+ in skeletal muscle interstitium during intense exercise has been suggested to cause fatigue in humans. The present study examined interstitial K+ kinetics and fatigue during repeated, intense, exhaustive exercise in human skeletal muscle. Ten subjects performed three repeated, intense (61.

Effect of high intensity training on capillarization and presence of angiogenic factors in human skeletal muscle.

The effect of intense training on endothelial proliferation, capillary growth and distribution of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) was examined in human skeletal muscle. Two intermittent knee extensor training protocols (at approximately 150% (Study 1) versus approximately 90% (Study 2) of leg (O(2) max)) were conducted. Muscle biopsies were obtained throughout the training periods for immunohistochemical assessment of capillarization, cell proliferation (Ki-67-positive cells), VEGF and bFGF.

The slow component of oxygen uptake during intense, sub-maximal exercise in man is associated with additional fibre recruitment.

Single muscle fibre metabolites and pulmonary oxygen uptake (VO2) were measured during moderate and intense, sub-maximal exercise to test the hypothesis that additional fibre recruitment is associated with the slow component of VO2. Seven healthy, male subjects performed 20 min moderate (MOD, approximately 50% of VO(2,max)) and intense (INT, approximately 80% VO(2,max)) cycling at 70 rpm. Glycogen content decreased significantly in type I and IIa fibres during INT, but only in type I fibres during MOD.

Effect of ribose supplementation on resynthesis of adenine nucleotides after intense intermittent training in humans.

The effect of oral ribose supplementation on the resynthesis of adenine nucleotides and performance after 1 wk of intense intermittent exercise was examined. Eight subjects performed a random double-blind crossover design. The subjects performed cycle training consisting of 15 x 10 s of all-out sprinting twice per day for 7 days.

Effects of high-intensity intermittent training on potassium kinetics and performance in human skeletal muscle.

A rise in extracellular potassium concentration in human skeletal muscle may play an important role in development of fatigue during intense exercise. The aim of the present study was to examine the effect of intense intermittent training on muscle interstitial potassium kinetics and its relationship to the density of Na(+),K(+)-ATPase subunits and K(ATP) channels, as well as exercise performance, in human skeletal muscle. Six male subjects performed intense one-legged knee-extensor training for 7 weeks.

Effect of high-intensity intermittent training on lactate and H+ release from human skeletal muscle.

The study investigated the effect of training on lactate and H+ release from human skeletal muscle during one-legged knee-extensor exercise. Six subjects were tested after 7-8 wk of training (fifteen 1-min bouts at approximately 150% of thigh maximal O2 uptake per day). Blood samples, blood flow, and muscle biopsies were obtained during and after a 30-W exercise bout and an incremental test to exhaustion of both trained (T) and untrained (UT) legs.

Match performance of high-standard soccer players with special reference to development of fatigue.

The aim of this study was to assess physical fitness, match performance and development of fatigue during competitive matches at two high standards of professional soccer. Computerized time-motion analyses were performed 2-7 times during the competitive season on 18 top-class and 24 moderate professional soccer players. In addition, the players performed the Yo-Yo intermittent recovery test.

Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism.

Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na+, K+, and pH homeostasis, were selected for analyses.

The yo-yo intermittent recovery test: physiological response, reliability, and validity.

Purpose: To examine the physiological response and reproducibility of the Yo-Yo intermittent recovery test and its application to elite soccer.

Methods: Heart rate was measured, and metabolites were determined in blood and muscle biopsies obtained before, during, and after the Yo-Yo test in 17 males. Physiological measurements were also performed during a Yo-Yo retest and an exhaustive incremental treadmill test (ITT).

Muscle interstitial potassium kinetics during intense exhaustive exercise: effect of previous arm exercise.

Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was examined. Possible microdialysis-induced rupture of the sarcolemma was assessed by measurement of carnosine in the dialysate, because carnosine is only expected to be found intracellularly.

ATP and heat production in human skeletal muscle during dynamic exercise: higher efficiency of anaerobic than aerobic ATP resynthesis.

The aim of the present study was to simultaneously examine skeletal muscle heat production and ATP turnover in humans during dynamic exercise with marked differences in aerobic metabolism. This was done to test the hypothesis that efficiency is higher in anaerobic than aerobic ATP resynthesis. Six healthy male subjects performed 90 s of low intensity knee-extensor exercise with (OCC) and without thigh occlusion (CON-LI) as well as 90 s of high intensity exercise (CON-HI) that continued from the CON-LI bout.

Activity profile and physiological demands of top-class soccer assistant refereeing in relation to training status.

To determine the movement patterns and physiological demands of top-class soccer assistant referees, we performed computerized time-motion analysis and measured heart rate and blood lactate concentration in 15 assistant referees during 22 competitive matches in the top Danish league. To relate match performance to the physical capability of the assistant referees, they performed a 3 x 30 m sprint protocol before and after matches and a laboratory treadmill test within 3 weeks of the games. The mean total distance covered by the top-class assistant referees was 7.

Localization and function of ATP-sensitive potassium channels in human skeletal muscle.

The present study investigated the localization of ATP-sensitive K+ (KATP) channels in human skeletal muscle and the functional importance of these channels for human muscle K+ distribution at rest and during muscle activity. Membrane fractionation based on the giant vesicle technique or the sucrose-gradient technique in combination with Western blotting demonstrated that the KATP channels are mainly located in the sarcolemma. This localization was confirmed by immunohistochemical measurements.