Publications by authors named "Bangfen Yan"
[Infectious bovine rhinotracheitis viral gG expression and gG-ELISA development].
Sheng Wu Gong Cheng Xue Bao
September 2007
Taking the genome DNA of Infectious Bovine Rhinotracheitis Virus (IBRV) as the template, the gG gene was amplified with PCR and cloned into the T cloning vector pMD18-T. After being identified by restriction digestion and DNA sequencing, the insert was subcloned into the expression vector pGEX-KG. Sodium docecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot assay showed that this gene was expressed as both soluble form and inclusion body by the transformed E.
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- - The fACE2 gene from domestic cat lungs was isolated using RT-PCR and found to be 2418 base pairs long, closely resembling human ACE2 compared to other non-primate animals.
- - A specific fragment of the gene (19-367 amino acids) was produced in E. coli for further study.
- - Tests showed that fACE2 effectively interacts with the SARS-CoV S1 protein, similar to the way human ACE2 does in Vero E6 cells.