[Detection of human papillomavirus (H.P.V.) DNA in genital lesions using molecular hybridization].

Detection of human papilloma virus in genitals lesions by molecular hybridization. Some H.P.

[Evaluation of molecular hybridization tests and DNA restriction enzyme analysis for the detection and typing of herpes simplex virus (HSV)].

Restriction enzyme analysis of DNA and blot hybridization tests were applied for the identification of 90 herpes simplex viruses isolated. The results obtained by those methods correlated completely with typing by monoclonal antibodies in an indirect immunofluorescent assay.

[Optimal detection of herpes simplex virus in clinical specimens preserved in transport media].

The sensitivity of the methods used for HSV detection depends upon the stage and the transport of the collected lesions. High detection rates are achieved with early vesicular lesions rather with ulcers and scabs . Since HSV is heat-labile and to avoid loss of infectivity, specimens should be collected in transport medium and inoculated as soon as possible on the cell cultures.

[Comparative study of immunologic techniques applied to the detection of herpes simplex virus (HSV)].

Indirect immunofluorescence (IF), immunoperoxidase (IP) and seroneutralization tests were compared for detection and identification or Herpes simplex virus (HSV), directly in tissue smears or in cells inoculated with clinical materials. Detection and clear differentiation between the two HSV serotypes were obtained using rabbit immunoglobulin cross-absorbed with heterologous virus antigen. Immunofluorescent staining with specific monoclonal antibodies is as sensitive and more rapid than standard Vero cell cultures, for the laboratory diagnosis of HSV.