Aromatase (CYP19) gene as a biomarker for detection of naphthalene and phenanthrene in Colombo to Mirissa coastal water in Sri Lanka.

Naphthalene (NAP) and phenanthrene (PHE) are prevalent Polycyclic Aromatic Hydrocarbons (PAHs) in the environment. High-Performance Liquid Chromatography (HPLC) analysis was performed on marine water samples (n = 57) collected from 19 locations. Molecular screening of the aromatase (CYP19) gene expression was examined using quantitative Reverse Transcriptase PCR (qRT-PCR).

Effect of Food Amounts on Larval Performance, Bacteriome and Molecular Immunologic Development during First-Feeding Culture of European Eel.

Production of European eel offspring has become a reality, but liquid diets during larval culture hold new challenges. This study focused on increasing food amounts without compromising well-being or healthy larvae-bacteria interactions. First-feeding larvae were fed two food amounts (Low = 0.

A high-fidelity, dual site-specific integration system in CHO cells by a Bxb1 recombinase.

Site-specific integration (SSI) via recombinase mediated cassette exchange (RMCE) has shown advantages over random integration methods for expression of biotherapeutics. As an extension of our previous work developing SSI host cells, we developed a dual-site SSI system having two independent integration sites at different genomic loci, each containing a unique landing pad (LP). This system was leveraged to generate and compare two RMCE hosts, one (dFRT) compatible with the Flp recombinase, the other (dBxb1) compatible with the Bxb1 recombinase.

Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets.

European eel (Anguilla anguilla) is a commercially important species for fisheries and aquaculture in Europe and the attempt to close the lifecycle in captivity is still at pioneering stage. The first feeding stage of this species is characterized by a critical period between 20 to 24 days post hatch (dph), which is associated with mortalities, indicating the point of no return. We hypothesized that this critical period might also be associated with larvae-bacterial interactions and the larval immune status.

The impact of expression vector position on transgene transcription allows for rational expression vector design in a targeted integration system.

Site-specific integration (SSI) technology has emerged as an effective approach by the pharmaceutical industry for the development of recombinant Chinese hamster ovary (CHO) cell lines. While SSI systems have been demonstrated to be effective for the development of CHO cell lines, they can be limiting in terms of both transgene expression and in the case of multi-specifics, the ability to generate the correct product of interest. To maximize the performance of Pfizer's dual SSI expression system for expressing monoclonal and multi-specific antibodies, we used a novel approach to investigate the positional effect of transgenes within expression vectors by engineering nucleotide polymorphisms (NP)s to use as biomarkers to track the level of transcript output from each expression vector position.

Exploring first-feeding diets for European eel larval culture: Insights at morphological, nutritional, and molecular levels.

Closing the life cycle of European eel (Anguilla anguilla) in captivity is targeted to provide a sustainable, year-round supply of juveniles for aquaculture. Present focus is on the nutritional requirements during the larval first-feeding period. In this study, three experimental diets were tested on hatchery-produced European eel larvae from the onset of the first-feeding stage commencing 10 days post hatch (dph) until 28 dph.

Learned uncertainty: The free energy principle in anxiety.

Generalized anxiety disorder is among the world's most prevalent psychiatric disorders and often manifests as persistent and difficult to control apprehension. Despite its prevalence, there is no integrative, formal model of how anxiety and anxiety disorders arise. Here, we offer a perspective derived from the free energy principle; one that shares similarities with established constructs such as .

Tropical and subtropical Asia's valued tree species under threat.

Tree diversity in Asia's tropical and subtropical forests is central to nature-based solutions. Species vulnerability to multiple threats, which affect provision of ecosystem services, is poorly understood. We conducted a region-wide, spatially explicit assessment of the vulnerability of 63 socioeconomically important tree species to overexploitation, fire, overgrazing, habitat conversion, and climate change.

Autonomous Surface and Underwater Vehicles as Effective Ecosystem Monitoring and Research Platforms in the Arctic-The Glider Project.

Effective ocean management requires integrated and sustainable ocean observing systems enabling us to map and understand ecosystem properties and the effects of human activities. Autonomous subsurface and surface vehicles, here collectively referred to as "gliders", are part of such ocean observing systems providing high spatiotemporal resolution. In this paper, we present some of the results achieved through the project "Unmanned ocean vehicles, a flexible and cost-efficient offshore monitoring and data management approach-GLIDER".

Insights on overflow from failure to report tasks.

Theories of consciousness diverge on the functional requirement that a conscious state need be reportable. Some maintain that the perceptual system's capacity for consciousness exceeds that of its capacity for access. Others contend that what is accessed is all there is to consciousness.

Two hundred years of zooplankton vertical migration research.

Vertical migration is a geographically and taxonomically widespread behaviour among zooplankton that spans across diel and seasonal timescales. The shorter-term diel vertical migration (DVM) has a periodicity of up to 1 day and was first described by the French naturalist Georges Cuvier in 1817. In 1888, the German marine biologist Carl Chun described the longer-term seasonal vertical migration (SVM), which has a periodicity of ca.

A Patient with Multiple Gastrointestinal Carcinoid Tumours Presenting with Jejunal Intussusception.

Carcinoid tumours are neuroendocrine tumours which arise from the enterochromaffin cells in the gastrointestinal and bronchopulmonary systems. The presentation of multiple gastrointestinal carcinoids with jejunal intussusception is rare, and the diagnosis may be challenging. A 49-year-old patient with adult onset bronchial asthma presented with pain around the umbilical region for 1-day duration.

Modified, optimized method of determination of Tributyltin (TBT) contamination in coastal water, sediment and biota in Sri Lanka.

Tributyltin (TBT) is a toxic organotin compound that belongs to the group of Persistent Organic Pollutants (POPs) and it is documented to cause severe sexual disorders development in aquatic fauna. According to the present study, The TBT concentration in coastal water ranged from 303 ± 7.4 ngL to 25 ± 4.

A Low-Cost NDIR-Based NO Gas Detection Device for Agricultural Soils: Assembly, Calibration Model Validation, and Laboratory Testing.

This research presents a low-cost, easy-to-assemble nondispersive infrared (NDIR) device for monitoring NO gas concentration in agricultural soils during field and laboratory experiments. The study aimed to develop a cost-effective instrument with a simple optic structure suitable for detecting a wide range of soil NO gas concentrations with a submerged silicone diffusion cell. A commercially available, 59 cm path-length gas cell, microelectromechanical systems (MEMS)-based infrared emitter, pyroelectric detector, two anti-reflective (AR) coated optical windows, and one convex lens were assembled into a simple instrument with secure preciseness and responsivity.

A Study of the Pattern of Admissions to the Accident and Emergency (A&E) Department of a Tertiary Care Hospital in Sri Lanka.

Introduction: The latest national healthcare reform policies of Sri Lanka include the development of accident and emergency (A&E) departments in all major hospitals. Provincial General Hospital Kurunegala (PGHK) is a home to the first established A&E department in Sri Lanka. PGHK provides services to a population of 2.

LSTM-MSNet: Leveraging Forecasts on Sets of Related Time Series With Multiple Seasonal Patterns.

Generating forecasts for time series with multiple seasonal cycles is an important use case for many industries nowadays. Accounting for the multiseasonal patterns becomes necessary to generate more accurate and meaningful forecasts in these contexts. In this article, we propose long short-term memory multiseasonal net (LSTM-MSNet), a decomposition-based unified prediction framework to forecast time series with multiple seasonal patterns.

Occurrence of Fungi and Fungal Toxins in Fish Feed During Storage.

Periods of unfavorable storing conditions can lead to changes in the quality of fish feeds, as well as the development of relevant mycotoxins. In the present study, a commercial fish feed was stored under defined conditions for four weeks. The main findings indicate that even storing fish feeds under unsuitable conditions for a short duration leads to a deterioration in quality.

tACS Stimulation at Alpha Frequency Selectively Induces Inattentional Blindness.

Contemporary neurocognitive models implicate alpha oscillations as a top-down mechanism of cortical inhibition, instrumental in the suppression of information that fails to reach conscious visual awareness. This suggests that alpha-band activity may play a key role in the phenomenon of inattentional blindness, however this has not yet been empirically examined. The current study employed transcranial alternating current stimulation (tACS) over occipital cortex at alpha, theta, and sham frequencies within an inattentional blindness task to delineate whether an exogenous manipulation of alpha oscillations has a modulatory effect on visual awareness of the unexpected stimulus.

Physician attire: physicians perspectives on attire in a community hospital setting among non-surgical specialties.

: Several studies have demonstrated a patient preference for physicians wearing a white coat associated with improved patient satisfaction. There are few studies on physicians' perceptions of attire mainly done in the outpatient and surgical specialties. : Assess non-surgical physicians' perception of attire in the hospital and to identify if any difference in the choice of attire amongst generation X and millennial physicians.

Searching for emotion: A top-down set governs attentional orienting to facial expressions.

Research indicates that humans orient attention toward facial expressions of emotion. Orienting to facial expressions has typically been conceptualised as due to bottom-up attentional capture. However, this overlooks the contributions of top-down attention and selection history.

A Rare Presentation of Coinfection: Dengue Virus and Hepatitis A Virus.

Dengue fever caused by dengue virus is a common tropical infection transmitted by the mosquitos and . Four strains of the genus flavivirus is responsible for the epidemics of varying severity. Hepatitis A caused by hepatitis A virus is spread by faecal-oral route.

Improvement of growth rates through nucleoside media supplementation of CHO clones.

Chinese Hamster Ovary (CHO) cells are used for the production of therapeutic proteins. This work examines improving passaging growth rate of two CHO clones. Growth rates were significantly improved for both clones with supplementation of the nucleosides cytidine, hypoxanthine, uridine, and thymidine to the culturing media at the optimal concentration of 100 µM of each nucleoside.

Comparison of Integrases Identifies Bxb1-GA Mutant as the Most Efficient Site-Specific Integrase System in Mammalian Cells.

Phage-derived integrases can catalyze irreversible, site-specific integration of transgenic payloads into a chromosomal locus, resulting in mammalian cells that stably express transgenes or circuits of interest. Previous studies have demonstrated high-efficiency integration by the Bxb1 integrase in mammalian cells. Here, we show that a point mutation (Bxb1-GA) in Bxb1 target sites significantly increases Bxb1-mediated integration efficiency at the Rosa26 locus in Chinese hamster ovary cells, resulting in the highest integration efficiency reported with a site-specific integrase in mammalian cells.