Potential in a single cancer cell to produce heterogeneous morphology, radiosensitivity and gene expression.

Morphologically heterogeneous colonies were formed from a cultured cell line (KYSE70) established from one human esophageal carcinoma tissue. Two subclones were separated from a single clone (clone13) of KYSE70 cells. One subclone (clone13-3G) formed mainly mounding colonies and the other (clone 13-6G) formed flat, diffusive colonies.

Clinicopathologic features of epidermal cysts of the sole: comparison with traditional epidermal cysts and trichilemmal cysts.

Background: It has been described that the etiology of epidermal cysts on acral skin is different from that on non-acral skin; however, no papers have been published regarding the detailed histological differences between acral and non-acral epidermal cysts. In this study, we compared the clinicopathologic findings of epidermal cysts of the sole with those of traditional epidermal cysts and trichilemmal cysts.

Methods: The cases studied were 12 epidermal cysts of the sole, 35 traditional (non-acral) epidermal cysts, and 12 trichilemmal cysts.

Cytokeratins 7 and 20 and mucin core protein expression in esophageal cervical inlet patch.

Cervical inlet patch (CIP) is defined by the presence of gastric mucosa within the first few centimeters of the esophagus. Several endoscopic series have demonstrated a frequent association of CIP with Barrett's esophagus (BE) suggesting a pathogenetic link. A histochemical study reporting the presence of acid mucin in CIP, including sulfomucin, supports this hypothesis.

Setting characteristics of silicone-based resilient denture liners.

The aim of this study was to investigate the setting characteristics of seven silicone-based resilient denture liners during setting. Maximum extrusion force--at the flow rate of 1 cm/min--ranged from 0.183 to 1.

Light-attenuating effect of dentin on the polymerization of light-activated restorative resins.

The light-attenuating effect of dentin on the mechanical properties of light-activated composite resins was evaluated using a simple experimental filter. The filter was designed to simulate the light transmittance and light diffusion characteristics of 1.0-mm thick dentin.

Adhesive strength of paint-on resins to crown and bridge composites.

This paper examined the adhesive strength of paint-on resin to crown and bridge composites after soaking in water and thermal-cycling. Three shades of paint-on resin were coated on three kinds of crown and bridge composite under four surface treatment conditions (a combination of sandblaster and pretreatment liquid). These specimens were soaked in water at 37 degrees C for 1 day, 1 month, and 1 year, and at 4 degrees C and 60 degrees C alternatively for 1-minute periods for 10,000 cycles by thermal-cycling machine.

Multicentric contiguous variant of epithelioid angiosarcoma of the bone. A rare variant showing angiotropic spread.

Epithelioid angiosarcoma of the bone is a rare tumor and is a diagnostic challenge. Here we present an autopsy case of a 62-year-old man with multifocal osteolytic lesions in the extremities and the pelvis. The initial diagnosis of a tibial biopsy was poorly differentiated adenocarcinoma.

Biocompatibility of composite membrane consisting of oriented needle-like apatite and biodegradable copolymer with soft and hard tissues in rats.

The purpose of this study was to evaluate histologically the reaction of soft and hard tissues in rats toward a composite membrane consisting of oriented needle-like apatite crystals and biodegradable copolymer. Histological findings regarding the soft tissue reaction showed that the membrane with or without apatite was covered by fibrous connective tissue at four weeks after implantation. In the case of hard tissue, the defect in dura mater--which was covered with an apatite-containing membrane--was fully filled with new bone at four weeks after implantation.

Radiosensitivity of peripheral blood lymphocytes obtained from patients with cancers of the breast, head and neck or cervix as determined with a micronucleus assay.

The in vitro radiation sensitivities of peripheral blood lymphocytes obtained from 48 normal females and 168 female cancer patients were measured with the cytokinesis-blocking micronucleus assay. Cancer patients group had significantly higher mean baseline micronucleus frequency than normal healthy controls. Breast cancer patients were more radiosensitive than normal individuals.

Bonding strength and durability of alkaline-treated titanium to veneering resin.

The shear bonding strengths of a veneering resin to polished, sandblasted, and retention bead-cast commercially pure titanium (cpTi) plates with and without alkaline treatment were measured before and after thermal cycling. The bonding strengths to polished cpTi with and without alkaline treatment decreased remarkably with thermal cycling (p<0.01).

Light-curing reinforcement for denture base resin using a glass fiber cloth pre-impregnated with various urethane oligomers.

The purpose of this study was to investigate the flexural properties of denture base resin reinforced using glass fiber cloth and a urethane oligomer. The five types of oligomer used in this study were S5, S9, S3, U4, and U6, which have varying functional groups and viscosities. The flexural properties of S9 with glass fiber cloth could not be measured because S9 is elastic.

An unusual form of crystal-forming chronic interstitial nephritis following long-term exposure to tosufloxacin tosilate.

Fluoroquinolones are known to cause acute renal failure because of interstitial nephritis with or without epithelioid granulomas. We report the first case of slowly progressive renal failure caused by crystal-forming chronic interstitial nephritis with non-Langerhans' cell histiocytosis after long-term exposure to a fluoroquinolone, tosufloxacin tosilate. Lesions consisted of spindle- to cuboidal-shaped histiocytes with minimal collagenous matrix and low-level lymphocyte infiltration replacing normal tubulointerstitial structure of the kidney.

Histopathologic aspects of photodynamic therapy for dysplasia and early adenocarcinoma arising in Barrett's esophagus.

The efficacy of photodynamic therapy (PDT) is currently evaluated for the treatment of superficial neoplasms arising in Barrett's esophagus (BE). An accurate assessment of this technique requires the evaluation of biopsies before and after treatment. However, despite the importance of pathology, only a limited number of studies have systematically assessed the mucosal changes after PDT.

Comparison of the mechanical properties of 2 prosthetic mini-implants.

Two prosthetic mini-implants (MTI and MDI), which have very similar shapes, are widely used. In this investigation, the mechanical and physical properties of 2 prosthetic mini-implants were investigated. The flexural properties were measured with a universal testing machine.

Churg-Strauss syndrome manifesting as perforation of the small intestine: report of a case.

We report a case of Churg-Strauss syndrome (CSS) causing perforation of the small intestine. A 51-year-old woman was admitted with an asthma attack and paralysis of both legs. Intravenous predonisolone (40 mg) was given to relieve her asthma.

Flexural properties of denture base polymers reinforced with a glass cloth-urethane polymer composite.

Objectives: A newly designed light-cured reinforcement made from urethanemethacrylate oligomer and woven glass cloth has orthotropic anisotropy. This is produced for incorporation into the outermost position under the greatest tension in denture base resins. In this study, the flexural properties of self-, heat-, and light-curing reinforced resins were determined.

Toothbrush abrasion of paint-on resins for shade modification and crown resins: effect of water absorption.

In order to investigate the clinical application of paint-on resins, the effect of water absorption on toothbrush abrasion and light transmittance of ten crown resins including three paint-on resins was examined. Water absorption into each material ranged from 0.29 to 0.

Optical and color stabilities of paint-on resins for shade modification of restorative resins.

The purpose of this study was to examine the optical and color stabilities of the paint-on resin used for shade modification of restorative resins. Three shades of paint-on resin and two crown and bridge resins were used. The light transmittance characteristics of the materials during accelerated aging tests such as water immersion, toothbrush abrasion, ultraviolet (UV) light irradiation, and staining tests were measured.

Taxonomic discrimination of flowering plants by multivariate analysis of Fourier transform infrared spectroscopy data.

Fourier transform infrared spectroscopy (FTIR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole cells are analyzed. Leaf samples of seven higher plant species and varieties were subjected to FTIR to determine whether plants can be discriminated phylogenetically on the basis of biochemical profiles. A hierarchical dendrogram based on principal component analysis (PCA) of FTIR data showed relationships between plants that were in agreement with known plant taxonomy.

Proteasome-dependent degradation of cyclin D1 in 1-methyl-4-phenylpyridinium ion (MPP+)-induced cell cycle arrest.

1-Methyl-4-phenylpyridinium ion (MPP(+)), an active metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, induces cell death and inhibition of cell proliferation in various cells. However, the mechanism whereby MPP(+) inhibits cell proliferation is still unclear. In this study, we found that MPP(+) suppressed the proliferation with accumulation in G(1) phase without inducing cell death in p53-deficient MG63 osteosarcoma cells.

Mucosa-associated lymphoid tissue-type lymphoproliferative lesion of the common bile duct.

Only a few cases of primary lymphoma of the common bile duct have been reported up to now. Here, we report a mucosa-associated lymphoid tissue (MALT)-type lymphoproliferative lesion of the common bile duct with some considerations about MALT lymphoma and benign lymphoproliferation. The patient, a 71-year-old woman, was admitted to our hospital because of progressive epigastric pain.

Real-time monitoring of apatite deposition using electrochemical quartz crystal microbalance.

The electrochemical deposition process of apatite was monitored in real-time using an electrochemical quartz crystal microbalance (EQCM). A piezoelectric quartz oscillator with titanium was fixed to a cell and connected to a frequency counter. The electrolyte temperature was maintained at 20, 37, and 60 degrees C and the direct current density was constant at 0.

Color change in acrylic denture base resin reinforced with wire mesh and glass cloth.

In this study, the L*a*b* color system as a color system and light transmittance of the denture base resin reinforced with wire mesh and glass cloth were measured, and the color difference (deltaE*ab) was calculated using L*, a* and b* values which were measured both on a white calibration plate and on a null background. The thicknesses of test specimens, which were reinforced with wire mesh and glass cloth 0.5 and 1.

Expression of trypsin-like proteases and protease nexin-1 in mdx mouse muscles.

In order to examine the possible participation of trypsin-like proteases in the onset and progress of muscular dystrophy, we investigated the expression of the trypsin-like protease in muscular tissues in mdx mice. We found that the mRNAs of several trypsin-like proteases, including hepsin and t-PA, were expressed in the muscular tissues of mdx mice, but at levels not significantly different from normal mice. Since the enzymatic properties of dystrypsin, a muscle trypsin-like protease activated before onset of the disease, are similar to those of thrombin, we investigated the expression pattern of thrombin in mdx mouse muscles.