Optimization of Silver Staining in Artificial Cytology Samples of Filamentous Fungi.

The diagnosis of respiratory fungal infections is often aided by cytopathological evidence of fungi in sputum or bronchoalveolar lavage fluid. Grocott's methenamine silver (GMS) stain, a special stain frequently used to detect fungi in cytological specimens, can be challenging to apply stably. There is a need for improved documentation of staining procedures for fungal detection in cytological specimens.

A Case of Invasive Rhinosinusitis Caused by Penicillium brasilianum.

Penicillium brasilianum is an environmental filamentous fungus with useful applications in biotechnology. However, human infections caused by P. brasilianum are rare and not well understood.

Trajectory clustering of immune cells and its association with clinical outcomes after aneurysmal subarachnoid hemorrhage.

Background And Purpose: The immune response following aneurysmal subarachnoid hemorrhage (aSAH) can exacerbate secondary brain injury and impact clinical outcomes. As the immune response after aSAH is a dynamic process, we aim to track and characterize immune cell trajectories over time to identify patterns associated with various clinical outcomes.

Methods: In this retrospective single-center study of patients with aSAH, we analyzed immune cell count trajectories, including neutrophil, monocyte, and lymphocyte counts, collected from day 1 to day 14.

Development of a sex-specific visceral fat area estimation using discrete multi-wavelength near-infrared spectroscopy measurements in Korean individuals.

Background/objectives: A high level of visceral fat area (VFA) is associated with obesity and cardiometabolic risk factors. VFA measured by computer tomography (CT) scan is accurate but has limitations for everyday use. Meanwhile, near-infrared (NIR) light penetrates the superficial layers of the human body so that fat content can be measured just as CT imaging measures fat accumulation.

Development of Saccharomyces cerevisiae accumulating excessive amount of glycogen and its effects on gut microbiota in a mouse model.

Saccharomyces cerevisiae accumulates glycogen, a hyperbranched glucose polymer with multiple bio-functionalities. In this study, mutants of S. cerevisiae that accumulate excessive amounts of glycogen were developed through UV mutagenesis.

Nationwide Cohort observational study on the safety and efficacy of COVID-19 vaccination in patients with Moyamoya disease.

The safety and efficacy of coronavirus disease 2019 (COVID-19) vaccination in patients diagnosed with Moyamoya disease (MMD) have not been established. Using National Health Insurance Service data, this study analyzed the occurrence of stroke-related events and mortality following COVID-19 vaccination among patients diagnosed with MMD from 2008 to 2020. Among 10,297 MMD patients, 296 (2.

Community dynamics and assembly is driven by environmental microbiota mediated by spatiotemporal distribution: The case of Daqu fermentation.

In almost all environments, microbial interaction is shaped by differences in environmental microbial transport, resulting in synergistic or antagonistic effects among community members. Unfortunately, the current understanding of how environmental microbiota affect spontaneous fermentation is very limited. Here, we selected Daqu workshops with different usage times (named X (60 years), Y (10 years), and Z (0 year)) as research model.

Digital health literacy: A concept analysis.

Objective: To elucidate the concept of digital health literacy by delineating its primary dimensions, origins and effects. Through this clarification, we seek to augment our understanding of the contemporary use of the concept of digital health literacy.

Methods: Rodgers's concept analysis was employed to investigate digital health literacy as a context-influenced concept evolving with technological progress.

Enhancing Fracture Toughness of Dental Zirconia through Incorporation of Nb into the Surface.

Background: Our previous study found that the addition of pentavalent cations like niobium (Nb) to yttria-stabilized zirconia increased fracture toughness but also raised the coefficient of thermal expansion (CTE), and opacity also increased undesirably. A new surface treatment is required to boost fracture toughness without altering CTE or translucency.

Methods: The surfaces of pre-sintered 3 mol% yttria-stabilized tetragonal zirconia polycrystal (3Y-TZP) and 4.

Superficial abdominal surgical site infection caused by Aspergillus welwitschiae: a case report.

Background: Aspergillus spp. are rare causes of surgical site infections (SSIs). Specifically, Aspergillus section Nigri, commonly identified as Aspergillus niger through morphological findings, has infrequently been reported as an abdominal SSI pathogen.

Incidence and risk factors of contrast-induced sialadenitis after therapeutic neuroendovascular procedures.

Background And Purpose: Swelling of the salivary glands, known as contrast-induced sialadenitis (CIS), is an adverse reaction to iodide contrast agents. However, the incidence and risk factors of CIS after therapeutic neuroendovascular procedures have not yet been established.

Materials And Methods: Demographic and procedural factors that may influence the development of CIS were retrospectively analyzed to identify the incidence and risk factors of this condition.

Prediction of contralateral progression in patients with bilateral nonhemorrhagic moyamoya disease following unilateral revascularization surgery.

Objective: This study was designed to identify predictive factors associated with substantial contralateral progression in adult patients with bilateral nonhemorrhagic moyamoya disease (MMD) who undergo revascularization surgery (RS) on one hemisphere.

Methods: The authors retrospectively analyzed 174 contralateral hemispheres of patients with bilateral nonhemorrhagic MMD (non-hMMD) who underwent RS on one side. The primary endpoint was defined as substantial contralateral progression requiring additional RS 6 months after the initial RS.

Comparative transcriptome analysis of persimmon somatic mutants () identifies regulatory networks for fruit maturation and size.

Bud sports in fruit crops often result in new cultivars with unique traits, such as distinct fruit size and color, compared to their parent plants. This study investigates the phenotypic differences and gene expression patterns in Tonewase and Ohtanenashi persimmon bud sports compared to those in their parent, Hiratanenashi, based on RNA-seq data. Tonewase is characterized by early maturation, whereas Ohtanenashi is noted for its larger fruit size.

New lineages of RNA viruses from clinical isolates of revealed by fragmented and primer-ligated dsRNA sequencing (FLDS) analysis.

is a species in the order Mucorales that is known to cause mucormycosis, but it is poorly understood as a host of viruses. Here, we examined 25 clinical strains of for viral infection with a conventional double-stranded RNA (dsRNA) assay using agarose gel electrophoresis (AGE) and the recently established fragmented and primer-ligated dsRNA sequencing (FLDS) protocol. By AGE, five virus-infected strains were detected.

The mechanism of FBKL4.010 produced tetramethylpyrazine in the liquid fermentation by comparative transcriptomic techniques.

Tetramethylpyrazine (TTMP) is considered a crucial flavor component in Moutai-flavored liquor. FBKL4.010 () is the dominant species found in Moutai-flavor Daqu, and this study aims to determine the mechanism by which produces TTMP during liquid fermentation of Moutai-flavor Daqu.

Clinical Outcomes of Clipping and Coil Embolization for Ruptured Intracranial Aneurysms Categorized by Region and Hospital Size: A Nationwide Cohort Study in Korea.

Background: To analyze the outcomes of clipping and coiling for ruptured intracranial aneurysms (RIAs) based on data from the National Health Insurance Service in South Korea, with a focus on variations according to region and hospital size.

Methods: This study analyzed the one-year mortality rates for patients with RIAs who underwent clipping or coiling in 2018. Coiling was further categorized into non-stent assisted coiling (NSAC) and stent assisted coiling (SAC).

Disentangling linkages between satellite-derived indicators of forest structure and productivity for ecosystem monitoring.

The essential biodiversity variables (EBV) framework has been proposed as a monitoring system of standardized, comparable variables that represents a minimum set of biological information to monitor biodiversity change at large spatial extents. Six classes of EBVs (genetic composition, species populations, species traits, community composition, ecosystem structure and ecosystem function) are defined, a number of which are ideally suited to observation and monitoring by remote sensing systems. We used moderate-resolution remotely sensed indicators representing two ecosystem-level EBV classes (ecosystem structure and function) to assess their complementarity and redundancy across a range of ecosystems encompassing significant environmental gradients.