Prognostic value of sternal lymphadenopathy on malignancy and survival in dogs undergoing splenectomy.

The purpose of this retrospective cohort study was to determine prevalence of sternal lymphadenopathy in a population of dogs undergoing splenectomy, and to evaluate if the prevalence was associated with splenic malignancy. In addition, the study investigated if survival in dogs diagnosed with hemangiosarcoma (HSA) differed between those with or without sternal lymphadenopathy at the time of surgery. Digital radiographs and medical records for dogs who underwent splenectomy from 2013 to 2016 where retrospectively reviewed.

The Experience of Partner Relationships for Male Survivors of Childhood Sexual Abuse: A Qualitative Synthesis.

Research has documented wide-ranging psychological impacts of childhood sexual abuse (CSA) for male survivors, but their experience of relationships is understudied. This qualitative review aimed to synthesize the qualitative literature concerning the experience of partner relationships for male CSA survivors. Electronic searches were conducted across PsycINFO, CINAHL, and PubMed, complemented by hand searches of references.

Evaluation of Fluoroscopic-Guided Closed Reduction versus Open Reduction of Sacroiliac Fracture-Luxations Stabilized with a Lag Screw.

Objective:  The aim of this study was to compare radiographic outcomes of open reduction versus fluoroscopic-guided closed reduction (FGCR) of sacroiliac fracture-luxations stabilized with a lag screw, as well as peri- and postoperative complications.

Study Design:  Medical records (2010-2015) and radiographs of dogs and cats diagnosed with sacroiliac fracture-luxation that underwent open reduction without fluoroscopic guidance ( = 24) or FGCR ( = 17) were retrospectively reviewed to assess sacroiliac fracture-luxation reduction and lag screw placement, and lag screw loosening on follow-up radiographs (range, 1-8 weeks postoperatively) when available. Peri- and postoperative complications were also recorded.

Social determinants of mental health.

A person's mental health and many common mental disorders are shaped by various social, economic, and physical environments operating at different stages of life. Risk factors for many common mental disorders are heavily associated with social inequalities, whereby the greater the inequality the higher the inequality in risk. The poor and disadvantaged suffer disproportionately, but those in the middle of the social gradient are also affected.

Hemilaminectomy for thoracolumbar Hansen Type I intervertebral disk disease in ambulatory dogs with or without neurologic deficits: 39 cases (2008-2010).

Objective: To describe prognostic factors, outcome, and time to recovery among ambulatory dogs having hemilaminectomy for Hansen Type I intervertebral disk disease.

Study Design: Retrospective case series.

Animals: Dogs (n = 38; 39 hemilaminectomies).

Tarsometatarsal arthrodesis using tarsometatarsal intramedullary pin stabilization.

Objective: To report the use of tarsometatarsal intramedullary pin stabilization for arthrodesis of the tarsometatarsal joint.

Study Design: Case series.

Animals: Dogs (n = 12) and cats (n = 2) with tarsometatarsal instability.

Partnership in employee health. A workplace health program for British Columbia Public Service Agency (Canada).

Objective: To evaluate the My Health Matters! (MHM) program, a multifaceted workplace intervention relying on education and awareness, early detection and disease management with a focus on risk factors for metabolic syndrome.

Participants: The MHM program was offered to 2,000 public servants working in more than 30 worksites in British Columbia, Canada.

Methods: The MHM program included a health risk assessment combined with an opportunity to attend an on-site screening and face-to-face call back visits and related on-site educational programs.

Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets.

Catechol-O-methyltransferase (COMT) inhibition is widely used to potentiate the effects of levodopa in Parkinson's disease but the effects of nigral dopaminergic cell loss and levodopa treatment on COMT activity are not known. The present study investigated the expression of COMT in the brain and liver of normal common marmosets, and animals treated with MPTP and those treated with levodopa to induce dyskinesia. Reverse transcript PCR demonstrated the expression of COMT mRNA in the liver, cortex and striatum of normal marmosets.

Ionic currents underlying the response of rat dorsal vagal neurones to hypoglycaemia and chemical anoxia.

A proportion of dorsal vagal neurones (DVN) are glucosensors. These cells respond to brief hypoglycaemia either with a K(ATP) channel-mediated hyperpolarization or with depolarization owing to an as yet unknown mechanism. K(ATP) currents are observed not only during hypoglycaemia, but also in response to mitochondrial inhibition.

Neuronal responses to transient hypoglycaemia in the dorsal vagal complex of the rat brainstem.

Several regions of the mammalian brain contain glucosensing neurones. In vivo studies have suggested that those located in the hypothalamus and lower brainstem are involved in glucoprivic feeding and homeostatic control of blood glucose. We have identified and characterized hypoglycaemia-sensitive neurones in the dorsal vagal complex of the brainstem using in situ hybridization, single-cell RT-PCR and whole-cell patch-clamp recordings from rat brainstem slices.

Laparoscopic assisted vaginal hysterectomy--190 cases: complications and training.

Laparoscopic assisted vaginal hysterectomy continues to gain popularity worldwide without evidence from any controlled trials of its superiority over existing techniques. There is some concern over complication rates particularly damage to the urinary tract and haemorrhage. The Royal College of Obstetricians and Gynaecologists has compiled a register of preceptors for advanced laparoscopic surgery, however there is nothing to prevent a surgeon without adequate training or experience from embarking upon any form of minimal access surgery.

Aerosol and lobar administration of a recombinant adenovirus to individuals with cystic fibrosis. II. Transfection efficiency in airway epithelium.

A phase I clinical trial was conducted in which recombinant adenovirus containing the cystic fibrosis trans-membrane regulator (CFTR) (Ad2/CFTR) was administered by bronchoscopic instillation or aerosolization to the lungs of cystic fibrosis (CF) patients. In this paper, we evaluate the efficiency of Ad2/CFTR-mediated transduction of bronchial airway cells. The ability of an Ad2/CFTR vector to transduce airway cells was first evaluated in patients to whom the vector was administered by bronchoscopic instillation.

Aerosol and lobar administration of a recombinant adenovirus to individuals with cystic fibrosis. I. Methods, safety, and clinical implications.

Cystic fibrosis (CF), an autosomal recessive disorder resulting from mutations in the cystic fibrosis trans-membrane conductance regulator (CFTR) gene, is the most common lethal genetic illness in the Caucasian population. Gene transfer to airway epithelium, using adenoviruses containing normal CFTR cDNA, leads to transient production of CFTR mRNA and, in some studies, to correction of the airway epithelial ion transport defect caused by dysfunctional CFTR. Inflammatory responses to the adenoviral vector have been reported, particularly at high viral titers.

T-plate fixation of distal radial closing wedge osteotomies for treatment of angular limb deformities in 18 dogs.

Objective: To describe the surgical technique and clinical results of treatment for forelimb angular limb deformities, secondary to premature distal radial or ulnar physeal closure, by using T-plate fixation of a distal radial closing wedge osteotomy in 18 dogs.

Study Design: Retrospective clinical study.

Sample Population: 18 client-owned dogs.

Cationic lipid-mediated CFTR gene transfer to the lungs and nose of patients with cystic fibrosis: a double-blind placebo-controlled trial.

Background: We and others have previously reported significant changes in chloride transport after cationic-lipid-mediated transfer of the cystic fibrosis transmembrane conductance regulator (CFTR) gene to the nasal epithelium of patients with cystic fibrosis. We studied the safety and efficacy of this gene transfer to the lungs and nose of patients with cystic fibrosis in a double-blind placebo-controlled trial.

Methods: Eight patients with cystic fibrosis were randomly assigned DNA-lipid complex (active) by nebulisation into the lungs followed 1 week later by administration to the nose.

Safety of a single aerosol administration of escalating doses of the cationic lipid GL-67/DOPE/DMPE-PEG5000 formulation to the lungs of normal volunteers.

Several groups are assessing the use of cationic lipids for respiratory gene therapy. To date no human data are available regarding the safety of intra-pulmonary cationic lipid delivery. In preparation for a trial of pulmonary delivery of the CFTR gene, we have assessed the safety of nebulised lipid GL-67/DOPE/DMPE-PEG5000 (GL-67A), the cationic lipid formulation to be used in this study.

Comparison of DNA-lipid complexes and DNA alone for gene transfer to cystic fibrosis airway epithelia in vivo.

Cationic lipids show promise as vectors for transfer of CFTR cDNA to airway epithelia of patients with cystic fibrosis (CF). However, previous studies have not compared the effect of DNA-lipid to DNA alone. Recently, we developed a formulation of plasmid encoding CFTR (pCF1-CFTR) and cationic lipid (GL-67:DOPE) that generated greater gene transfer in mouse lung than previously described DNA-lipid vectors.

Repeat administration of an adenovirus vector encoding cystic fibrosis transmembrane conductance regulator to the nasal epithelium of patients with cystic fibrosis.

Cystic fibrosis (CF) is a common autosomal recessive disease caused by mutations in the CF transmembrane conductance regulator gene. Recombinant adenoviruses have shown promise as vectors for transfer of CF transmembrane conductance regulator cDNA to airway epithelia and correction of the Cl- transport defect. However, because adenovirus-mediated gene transfer is transient, use of adenovirus as a vector for treatment of CF would require repeated administration.

Multiplex PCR amplification from the CFTR gene using DNA prepared from buccal brushes/swabs.

Traditionally, DNA used for PCR-based diagnostic analysis has originated from white cells fractionated from whole blood. Although this method yields substantial quantities of DNA, there are some drawbacks to the procedure, including the inconvenience of drawing blood, risk of exposure to blood-borne pathogens, liquid sample handling, and the somewhat involved extraction procedure. Alternatively, DNA for genetic diagnosis has been derived from finger stick blood samples, hair roots, cheek scrapings, and urine samples.