Expression of Macrophage Colony-Stimulating Factor CSF-1 in Spinal Cord Neurons in Mice with Experimental Autoimmune Encephalomyelitis.

In mice with acute and chronic models of experimental autoimmune encephalomyelitis (EAE), a quantitative study of the dynamics of CSF-1 and IL-34 protein levels in the spinal cord and blood plasma was conducted by ELISA and the specificity of CSF-1 expression in spinal cord cells was examined by immunohistological methods. A significant increase in the level of CSF-1 in the spinal cord was detected and populations of motoneurons with intense CSF-1 immunoreactivity were identified in mice with acute and chronic EAE. The obtained results suggest a possible role of CSF-1 in the pathogenesis and progression of EAE/multiple sclerosis.

CRDS line-shape study of the (7-0) band of CO.

We present the results of the spectral line-shape study of the first measurement of the extremely weak (7-0) band of the CO molecule. Measurements were done with a highly sensitive cavity ring-down spectrometer. Collisional narrowing, analyzed in terms of speed-dependent effects, was observed for the first time for transitions with line intensities below 2⋅10 cm/molecule at 296 K.

Pressure and temperature dependencies of air-perturbed O B-band line shapes.

The air-broadened lines from the oxygen B band were measured for the first time in controlled laboratory conditions with a high signal-to-noise ratio using frequency-stabilized cavity ring-down spectroscopy (FS-CRDS) referenced to the optical frequency comb. Spectra measured at various pressures and temperatures were analyzed with an advanced line-shape model, considering the speed-dependence of collisional broadening and shift, and the effect of velocity-changing collisions. The temperature dependence of collisional broadening and shift is determined, whereas no significant temperature dependence of speed-dependent parameters and Dicke narrowing was observed.

Measurement and calculation of CO (7-0) overtone line intensities.

Intensities of 14 lines in the sixth overtone (7-0) band of carbon monoxide (12C16O) are measured in the visible range between 14 300 and 14 500 cm-1 using a frequency-stabilized cavity ring-down spectrometer. This is the first observation of such a high and weak overtone spectrum of the CO molecule. A theoretical model is constructed and tested based on the use of a high accuracy ab initio dipole moment curve and a semi-empirical potential energy curve.

Composite Lamina Model Design with the Use of Heuristic Optimization.

In engineering practice, a problem is quite often faced in which the number of unknown parameters exceeds the number of conditions or requirements or, otherwise, there are too many requirements for too few parameters to design. Such under- or over-defined tasks are sometimes not possible to solve using a direct approach. The number of solutions to such problems is multiple, and it is most rational to search for the optimal one by numerical methods since the more unknown design parameters there are to be designed, the more potential solutions there are.

Pure rotational R(0) and R(1) lines of CO in Ar baths: experimental broadening, shifting and mixing parameters in a wide pressure range calculations.

The results of a rigorous study of the two first pure rotational transitions of CO perturbed by Ar are presented. The experimental part is based on the use of three different spectrometers covering together the pressure range from 0.02 up to 1500 torr.

Changes in the Neuronal Population of the Spinal Cord of Mice with Experimental Autoimmune Encephalomyelitis as a Model of Multiple Sclerosis.

We performed a quantitative study of the neuronal population in the spinal cord of mice with acute and chronic model of experimental autoimmune encephalomyelitis. Immunohistological/immunofluorescent analysis with motor neuron marker ChAT revealed a significant decrease in the number of motor neurons in the ventral horns of the lumbar spinal cord in the acute form of autoimmune encephalomyelitis, with the further appearance of large empty (containing no motor neurons) areas in the ventral horns in the chronic form. The development of experimental autoimmune encephalomyelitis is accompanied by degradation and death of neurons, in particular ChAT motor neurons.

The 4D Nucleome Data Portal as a resource for searching and visualizing curated nucleomics data.

The 4D Nucleome (4DN) Network aims to elucidate the complex structure and organization of chromosomes in the nucleus and the impact of their disruption in disease biology. We present the 4DN Data Portal ( https://data.4dnucleome.

Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications.

For quality, interpretation, reproducibility and sharing value, microscopy images should be accompanied by detailed descriptions of the conditions that were used to produce them. Micro-Meta App is an intuitive, highly interoperable, open-source software tool that was developed in the context of the 4D Nucleome (4DN) consortium and is designed to facilitate the extraction and collection of relevant microscopy metadata as specified by the recent 4DN-BINA-OME tiered-system of Microscopy Metadata specifications. In addition to substantially lowering the burden of quality assurance, the visual nature of Micro-Meta App makes it particularly suited for training purposes.

Dynamics of Nafion membrane swelling in HO/DO mixtures as studied using FTIR technique.

Experiments with Fourier transform spectrometry of Nafion, a water-swollen polymeric membrane, are described. The transmittance spectra of liquid samples and Nafion, soaked in these samples, were studied, depending on the deuterium content in water in the spectral range 1.8-2.

[The safety of selective serotonin reuptake inhibitors: hemostasis].

The literature data of the reaction of haemostatic system to antidepressants from the group of selective inhibitors of serotonin reuptake are summarized. The development of haemorrhagic complications is analyzed. The risk/benefit ratio is estimated for the treatment of depression in patients with acute myocardial infarction and stroke.

[GABA-system and alcohol: does an "ethanol receptor" exist? (review)].

There are two alternative points of view in the current literature addressing the mechanisms of ethyl alcohol effects on cells. A historically first appeared hypothesis of ethanol influence on living systems is based on the physical-chemical background and described the membrane-associated and intracellular biochemical events as a consequence of changes in the membrane state. In opposite, the attempts exist to differentiate the "specific action" of alcohol on separate neurochemical systems.

[The role of diffuse endocrine system and colonocytes cellular renovation in formation of clinical variants of irritable colon syndrome in young persons].

60 patients with irritable colon syndrome (ICS) were examined. They were divided into 2 groups. Group 1 included 30 patients who had ICS with dominating constipation (ICSc).

[Allosteric regulation of receptors. I. Cooperative interaction and functional regulation].

Today thousands of scientific publications in the field of receptology are dedicated to allosteric regulation of receptors. Biochemical events in cardiovascular, nerve, immune, endocrine, and other systems are based on various allosteric processes. Many of pharmaceuticals exert their action via allosteric regulation of receptive structures.

[An integral chip for the multiphase pulse-duration modulation used for voltage changer in biomedical microprocessor systems].

An integral chip (IC) was designed for controlling the step-down pulse voltage converter, which is based on the multiphase pulse-duration modulation, for use in biomedical microprocessor systems. The CMOS technology was an optimal basis for the IC designing. An additional feedback circuit diminishes the output voltage dispersion at dynamically changing loads.

[The endogenous allosteric regulators of receptors].

The literature data devoted to endogenous allosteric regulators of membrane bound receptors are summarized in the present review. The allosteric processes are classified to (i) cooperative interaction, (ii) nonspecific, (iii) functional, and (iv) specific regulations according to target topography in a receptor. The specific endogenous allosteric regulators are described for GABAA, NMDA, muscarinic, nicotinic, serotonin, and opioid receptors.

[Regulation of opioid receptors by thyroliberin analogues].

The ability of thyroliberin (TRH) to interact with opioid receptors (OR) was studied using radioligand analysis. TRH did not influence specific binding of [3H]-naloxone, but increased affinity of high affinity binding sites for the ligand in a dose-dependent manner. TRH also decreased affinity of low- affinity binding sites.

[Medical informatics in the training of students, dentists and teachers in dental schools].

Philosophy of training students, physicians, and tutors of dentistry schools in medical informatics and the relevant curricula are discussed. Specialized software for differential diagnosis and treatment of the major stomatological diseases (dental caries, pulpitis, periodontitis, mucosal, periodontal, oral diseases, tumors, rapid methods for AIDS screening, etc.) are presented.