ACS Meas Sci Au
February 2025
A new triazine containing pyridinium organic polymer () based on 4-amino-1,8-naphthalimide Tröger's base is developed and employed as a fluorescent chemosensor for selective sensing of organoarsenic feed additives in water. was readily synthesized by reacting "V-shaped" bis-[-(4-pyridyl)methyl)]-9,18-methano-1,8-naphthalimide-[][1,5]diazocine with cyanuric chloride in DMF at 100 °C for 3 days. exhibited a strong fluorescence emission in water and displayed selective, reversible fluorescence sensing responses for the roxarsone feed additive at the nanomolar (19 nM) level of sensitivity.
View Article and Find Full Text PDFRHIM is a protein motif facilitating the assembly of large signaling complexes triggering regulated cell death. A few DNA viruses employ viral RHIMs mimicking host RHIMs and counteract cell death by interacting with host RHIM-proteins to alleviate antiviral defenses. Whether RNA viruses operate such viral RHIMs remains unknown.
View Article and Find Full Text PDFFront Endocrinol (Lausanne)
November 2024
Introduction: Diabesity, characterized by obesity-driven Type 2 diabetes mellitus (T2DM), arises from intricate genetic and environmental interplays that induce various metabolic disorders. The systemic lipid and glucose homeostasis is controlled by an intricate cross-talk of internal glucose/insulin and fatty acid molecules to maintain a steady state of internal environment.
Methods: In this study, were maintained to achieve glucose concentrations resembling the hyperglycemic conditions in diabetic patients to delve into the mechanistic foundations of diabesity.
The innate immune signals are the front line of host defense against bacterial pathogens. Pathogen-induced harmful effects, such as reduced neuronal signals to the intestine, affect the host's food sensing and dwelling behavior. Here, we report that dopamine and kpc-1 signals control the intestinal innate immune responses through the p38/PMK-1 MAPK signaling pathway in C.
View Article and Find Full Text PDFUltraviolet radiation is a non-ionizing radiation produced by longer wavelength energy sources with lower frequency and is categorized into UV-A, UV-B, and UV-C. Minimal exposure to this radiation has several health benefits, which include treating microbial contaminations and skin therapies. However, the antimicrobial action of low-dose UV-A during pathogenic bacterial infections is still unrevealed.
View Article and Find Full Text PDFIntroduction: The mobile phone has evolved into an indispensable accessory carried by everyone. With its increasing usage, there is a parallel rise in mobile phone addictions. Nomophobia, short for bile phone , is characterized as a fear specific to the absence of mobile phones.
View Article and Find Full Text PDFP-glycoprotein (P-gp) mediated multidrug resistance (MDR) is the leading cause of chemotherapy failure since it causes the efflux of chemotherapeutic drugs from the cancer cells. Solasodine, a steroidal alkaloid and oxaspiro compound, present in the Solanaceae family showed significant cytotoxic effects on various cancer cells. However, the effect of solasodine on reversing P-gp mediated drug resistance is still unknown.
View Article and Find Full Text PDFKlebsiella aerogenes (previously known as Enterobacter aerogenes) is a common opportunistic pathogen that infect the respiratory tract and central nervous system. However, how it interferes the host regulatory mechanism has not been previously described. When C.
View Article and Find Full Text PDFLoss of BRCA2 (breast cancer 2) is lethal for normal cells. Yet it remains poorly understood how, in BRCA2 mutation carriers, cells undergoing loss of heterozygosity overcome the lethality and undergo tissue-specific neoplastic transformation. Here, we identified mismatch repair gene mutL homolog 1 (MLH1) as a genetic interactor of BRCA2 whose overexpression supports the viability of Brca2-null cells.
View Article and Find Full Text PDFKlebsiella aerogenes, previously known as Enterobacter aerogenes, is a gram-negative bacterium typically present in the gastrointestinal tract. While numerous studies reported the pathogenicity and drug resistance of this bacterium there remains a lack of comprehensive research on K. aerogenes induced alterations in the host cellular mechanisms.
View Article and Find Full Text PDFPolymorphism is common in both in vitro and in vivo amyloid fibrils formed by the same peptide/protein. However, the differences in their self-assembled structures at the amino acid level remain poorly understood. In this study, we utilized isotope-edited vibrational circular dichroism (VCD) on a well-known amyloidogenic peptide fragment (NFGAIL) of human islet amyloid polypeptide (IAPf) to investigate the structural polymorphism.
View Article and Find Full Text PDFThe interactive network of hosts with pathogenic microbes is still questionable. It has been hypothesized and reported that the host shows altered regulatory mechanisms for different pathogens. Several studies using transcriptomics and proteomics revealed the altered pathways and sequential regulations displayed by the host during bacterial interactions.
View Article and Find Full Text PDFThe WNT signaling pathway is a central regulator of bone development and regeneration. Functional alterations of WNT ligands and inhibitors are associated with a variety of bone diseases that affect bone fragility and result in a high medical and socioeconomic burden. Hence, this cellular pathway has emerged as a novel target for bone-protective therapies, e.
View Article and Find Full Text PDFMetastatic progression of epithelial cancers can be associated with epithelial-mesenchymal transition (EMT) including transcriptional inhibition of E-cadherin (CDH1) expression. Recently, EM plasticity (EMP) and E-cadherin-mediated, cluster-based metastasis and treatment resistance have become more appreciated. However, the mechanisms that maintain E-cadherin expression in this context are less understood.
View Article and Find Full Text PDFAn attempt has been made to promote the efficiency of the electrochromic (EC) windows to perform at a faster switching rate with good coloration and easy recyclability. In this work, ion-assisted pulsed DC unbalanced confocal magnetron sputtering is used to fabricate mixed metal oxide thin films of Nb and Ti (which are termed as NTO) for EC applications. Further, to increase the EC efficiency of this film, a very thin metallic seed layer is incorporated between the substrate and the film using the layer-by-layer (LBL) coating strategy.
View Article and Find Full Text PDFBoosting catalytic performance as a vital role for an electrochemical sensor for monitoring various hazardous nitro drugs. Herein, an inexpensive, facile, and eco-friendly construction of praseodymium tungstate decorated on three dimensional porous biocarbon (PrW/3D-PBC) for electrochemical determination of carcinogenic residue furazolidone (FZ). The nanostructured PrW nanoparticles were prepared by solvent evaporation from peroxo-tungstic acid and 3D-PBC was prepared from biomass precursor under the carbonization method.
View Article and Find Full Text PDFIn this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utilization of ImageJ to evaluate larval dimensions and to explore the model in the context of hyperlipidemia. Researchers should be aware of context specificity of the proposed protocols and interpret results accordingly.
View Article and Find Full Text PDFCronobacter sakazakii, an opportunistic foodborne pathogen prevalently detected in contaminated powdered infant formula, is associated with different diseases, including meningitis. It can cross the blood-brain barrier and affects the CNS. The impact of C.
View Article and Find Full Text PDFNovel methods of sensing and treatment required to elicit potent humoral and cellular immune responses. Here, Streptavidin functionalized α-FeO-Au nanoparticles (STV-Mag) loaded cationic carbomate cholesterol is used as a carrier to release antibacterial thymol drug for Staphylococcus aureus (S. aureus) infected Caenorhabditis elegans (C.
View Article and Find Full Text PDFThe application of CRISPR has greatly facilitated genotype-phenotype studies of human disease models. In this protocol, we describe CRISPR-Cas9-induced gene knockout in zebrafish, utilizing purified Cas9 protein and -transcribed sgRNA. This protocol targets the PHLPP1 gene in an Indian wild-caught strain, but is broadly applicable.
View Article and Find Full Text PDFThat reversible protein phosphorylation by kinases and phosphatases occurs in metabolic disorders is well known. Various studies have revealed that a multi-faceted and tightly regulated phosphatase, pleckstrin homology domain leucine-rich repeat protein phosphatase (PHLPP)-1/2 displays robust effects in cardioprotection, ischaemia/reperfusion (I/R), and vascular remodelling. PHLPP1 promotes foamy macrophage development through ChREBP/AMPK-dependent pathways.
View Article and Find Full Text PDFThe purpose of this study was to examine whether the Cronobacter sakazakii infection-induced inflammation alters the Reelin signaling pathway that is involved in learning and memory. To test this, postnatal day (PND)-15 rat pups were either treated with Luria Bertani broth/Escherichia coli OP50/C. sakazakii through oral gavage or maintained as control and allowed to stay with their mothers until PND-24.
View Article and Find Full Text PDFInconel-718 (IN-718) is a commonly used nickel-based superalloy in various fields, such as gas turbine and power generation applications. However, the lower wear and oxidation resistance hinder their wide usage. In this work, FeCoCrNiMn particles were mechanically ball-milled and preplaced on the IN-718 substrate.
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