Protective Potential of Leaf Extract on Metabolic Disorders and Oxidative Stress in Model Animals.

Metabolic disorders (MDs) include disease states such as diabetes mellitus, obesity, dyslipidemia, hyperuricemia, etc., affecting about 30% of the planet's population. The purpose of the present study was to investigate the protective potential of leaf extract (ECA) against chemically induced type 2 diabetes in Wistar rats.

: Towards In-Depth Metabolite Profiling, Antioxidant and Enzyme-Inhibitory Potential.

L'Hèr. (Geraniaceae) is renowned for its traditional use as a flavor, ornamental and medicinal plant. This work aimed at an in-depth study of the phytochemical profiling and in vitro antioxidant and enzyme inhibition assessment of a methanol-aqueous extract from leaves.

Mitigating Effects of L. on Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD).

The metabolic syndrome and its associated co-morbidities have been recognized as predisposing risk factors for the development of metabolic-associated fatty liver disease (MAFLD). The present study reports on the beneficial effect of the methanol-aqueous extract (ETB) at 150 and 300 mg/kg bw on biochemical parameters related to oxidative stress, metabolic syndrome, and liver function in rat animal models with induced MAFLD. ETB was found to be non-toxic with LD50 > 3000 mg/kg and did not affect cell viability of hepatic HEP-G2 cells in a concentration up to 800 μg/mL.

A Comprehensive Phytochemical Analysis of Infusion Using Orbitrap UHPLC-HRMS.

Griseb, also known as "mountain tea" and "Olympus tea" (Lamiaceae family) is an endemic plant from the mountainous regions of the Balkan Peninsula. In this study, we focused on an in-depth phytochemical analysis of infusion using ultra-high-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UHPLC-HRMS). Quantitative determination of the main secondary metabolites was carried out by UHPLC-HRMS analyses using the external standard method.

The Phytochemical Profiling, In Vitro Antioxidant, and Hepatoprotective Activity of L. and Caffeoylquinic Acids in Diclofenac-Induced Hepatotoxicity on HEP-G2 Cells.

Oxidative stress is a common phenomenon of many liver disorders; it both affects patient survival and directly influences the applicability, effectiveness, and toxicity of drugs. In the pursuit of reliable natural remedies for hepatoprotection, this study reports on the complete phytochemical characterization, antioxidant, and hepatoprotective activities of the methanol-aqueous extract in an in vitro model of diclofenac-induced liver injury (DILI). An ultra high-performance liquid chromatography-high-resolution mass spectrometry analysis (UHPLC-HRMS) was conducted, delineating more than 100 secondary metabolites for the first time in the species, including a series of phenolic acid-hexosides, acylquinic, acylhydroxyquinic and acyltartaric acids, and flavonoids.

Phytochemical Profiling, Antioxidant and Cognitive-Enhancing Effect of ssp. (Roth) G. Don (Asteraceae).

This study aimed at the evaluation of the antioxidant and cognitive-enhancing effect of methanol-aqueous extract from ssp. aerial parts. Significant radical scavenging activity (110.

Antioxidant and Hepatoprotective Potential of L. Extracts on Induced Oxidative Stress In Vitro/In Vivo.

L. (Asteraceae) is traditionally used in the treatment of bacterial/fungal infections and respiratory and heart ailments. The aim of this study was to evaluate the potential of extracts from leaves (ERLE) and flowering heads (ERFE) as antioxidant and hepatoprotective agents on diclofenac-induced lipid peroxidation and oxidative stress under in vitro and in vivo conditions.

Metabolite profiling and bioactivity of (L.) Wallr. (Asteraceae, Cichorieae).

(L.) Wallr. is a perennial herbaceous plant in the tribe Cichorieae (Lactuceae), Asteraceae family, distributed in the mountainous regions in Europe.

Chemophenetic Approach to Selected Senecioneae Species, Combining Morphometric and UHPLC-HRMS Analyses.

Herein, a chemophenetic significance, based on the phenolic metabolite profiling of three (, and ) and two species ( and ), coupled to morphometric data, is presented. A set of twelve morphometric characters were recorded from each plant species and used as predictor variables in a linear discriminant analysis (LDA) model. From a total 75 observations (15 from each of the five species), the model correctly assumed their species' membership, except for 2 observations.

An In-Depth Study of Metabolite Profile and Biological Potential of L. (Costmary).

Asteraceae species Tanacetum balsamita L. (costmary) is renowned for its traditional usage as an aromatic, carminative and tonic plant. This work aimed at in-depth study of the phytochemical and in vitro biological profilings of methanol−aqueous extracts from the costmary leaves, flower heads and roots.

Antimicrobial and Antioxidant Potential of Microalgae in the Context of Integral Biorefinery Concept.

Small-scale photobioreactors (PBRs) in the inoculum stage were designed with internal (red or green) and external white LED light as an initial step of a larger-scale installation aimed at fulfilling the integral biorefinery concept for maximum utilization of microalgal biomass in a multifunctional laboratory. The specific growth rate of (Turpin) Kützing biomass for given cultural conditions was analyzed by using MAPLE software. For the determination of total polyphenols, flavonoids, chlorophyll "a" and "b", carotenoids and lipids, UHPLC-HRMS, ISO-20776/1, ISO-10993-5 and CUPRAC tests were carried out.

Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle ( Griseb.), Asteraceae.

The widespread genus Mill. (Asteraceae) is renowned in traditional medicine. In the present study, an innovative biochemometric-assisted metabolite profiling of the flower heads, aerial parts and roots of Griseb.

UHPLC-Orbitrap-MS Tentative Identification of 51 Oleraceins (Cyclo-Dopa Amides) in L. Cluster Analysis and MS Filtering by Mass Difference.

Oleraceins are a class of indoline amide glycosides found in L. (Portulacaceae), or purslane. These compounds are characterized by 5,6-dihydroxyindoline-2-carboxylic acid -acylated with cinnamic acid derivatives, and many are glucosylated.

Molecular networking-assisted flavonoid profile of extract in relation to its protective effects on carbon tetrachloride-induced hepatorenal damage in rats.

The aim of the study was to provide an in-depth characterization of the methanol-aqueous extract from the aerial parts of Pall. Ex Adams (Caryophyllaceae) (EGG) and to assess its protective potential on carbon tetrachloride (CCl)-induced liver and kidney damage in male Wistar rats. Twenty-two flavonoid , - and --glycosides in EGG were annotated by mass spectrometry--based molecular networking; nine of them are reported in this species for the first time.

In vivo toxicity assessment of Clinopodium vulgare L. water extract characterized by UHPLC-HRMS.

Clinopodium vulgare L. (Lamiaceae) was used in the traditional Bulgarian medicine for treatment of wounds, diabetes and gastric ulcers. In this study we aimed at safety assessment of C.

Gypsophila saponins enhance the cytotoxicity of etoposide in HD-MY-Z lymphoma cells.

Glucuronide Oleanane-type Triterpenoid Carboxylic Acid 3,28-Bidesmosides (GOTCAB) are accumulated in Gypsophila L. roots. In the study we aimed at investigating the possible synergistic effects of Gypsophila trichotoma GOTCABs and cytostatic etoposide towards the Hodgkin lymphoma cell line HD-MY-Z.

Trans-3,5-dicaffeoylquinic acid from Geigeria alata Benth. & Hook.f. ex Oliv. & Hiern with beneficial effects on experimental diabetes in animal model of essential hypertension.

Geigeria alata Benth. & Hook.f.

[Comparative evaluation of depressive disorders in women and men].

Aim: To study clinical characteristics of depression in women and men.

Material And Methods: One hundred and twenty women, aged from 18 to 65 years, and 67 age-matched men with the diagnosis of recurrent depressive disorder (RDD) (ICD-10 F33) were examined using a specially developed survey.

Results: Differences in the clinical picture and course of RDD in women and men were found.

A new liquid chromatography-high resolution Orbitrap mass spectrometry-based strategy to characterize Glucuronide Oleanane-type Triterpenoid Carboxylic Acid 3, 28-O-Bidesmosides (GOTCAB) saponins.A case study of Gypsophila glomerata Pall ex M. B. (Caryophyllaceae).

Glucuronide Oleanane-type Triterpenoid Carboxylic Acid 3, 28-Bidesmosides (GOTCAB) saponins are bioactive natural compounds spread in Caryophyllidae. The high complexity of GOTCAB occurring as closely related isobaric and positional isomers is a challenge in their separation and identification. A new liquid chromatography - high resolution Orbitrap mass spectrometry acquisition strategy would be important for the structural elucidation of GOTCAB in plant extracts.

Chemical characterization with in vitro biological activities of Gypsophila species.

Methanol-aqueous extracts from the aerial parts of Gypsophila glomerata (GGE), G. trichotoma (GTE) and G. perfoliata (GPE) were investigated for antioxidant potential using different in vitro models, as well as for phenolic and flavonoid contents.

[The differential diagnosis and treatment of depressive disorders in climacteric transition].

Aim: To study clinical symptoms of menopause depression, develop differential diagnostic criteria and consider treatment aspects.

Material And Methods: One hundred and fifty women, aged 41-65 years, with mental disorders developed in different periods of menopause (perimenopause, menopause and post menopause) were studied.

Results And Conclusion: Four types of depression were diagnosed: climacteric (n=46), psychogenic (n=35), endogenic (n=42) and involutional (n=27).

HPLC-UV and LC-MS Analyses of Acylquinic Acids in Geigeria alata (DC) Oliv. & Hiern. and their Contribution to Antioxidant and Antimicrobial Capacity.

Introduction: Geigeria alata is a traditional plant used in Sudanese folk medicine for treatment of diabetes, cough, epilepsy and intestinal complaints.

Objective: To analyze phenolic acids in Geigeria alata roots and leaves and to evaluate their antioxidant and antimicrobial activities.

Methodology: Phenolic acids in the aqueous-methanol extracts were identified by LC-MS.

Chemometrics-based Approach in Analysis of Arnicae flos.

Introduction: Arnica montana flowers have a long history as herbal medicines for external use on injuries and rheumatic complaints.

Objective: To investigate Arnicae flos of cultivated accessions from Bulgaria, Poland, Germany, Finland, and Pharmacy store for phenolic derivatives and sesquiterpene lactones (STLs).

Materials And Methods: Samples of Arnica from nine origins were prepared by ultrasound-assisted extraction with 80% methanol for phenolic compounds analysis.

[Hyperkinetic symptoms in children and adolescents with psychosomatic disorders].

Authors studied 151 patients with psychosomatic diseases with attention deficit of different severity and hyperkinetic disorders. Based on the results obtained in the study, it was suggested an affective- disontogenetic conception of hyperkinetic disorders in children and adolescents. A significant role of both biological and psychosocial factors as well as gender and age differences in the development and formation of activity and attention disorders is emphasized.

[The clinical picture and therapeutic principles in mental disorders in women with a postoperative climacteric].

49 patients after ovariectomy (OE) and 26 women after hysterectomy (HE) were examined. Psychiatrist's care was necessary for all of them because of marked mental disorders. The development of asthenic and dysthymic disorders was most typical after OE.