Quantification of tolvaptan in rabbit plasma by LC-MS/MS: Application to a pharmacokinetic study.

A sensitive, selective and high-throughput liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the quantitation of tolvaptan in rabbit plasma. Sample clean-up involved liquid-liquid extraction (LLE) and chromatography was performed on Zorbax SB C analytical column (50 mm×2.1 mm, 3.