[On the 100th anniversary of the Department of Otorhinolaryngology of Burdenko Voronezh State Medical University].

The article is dedicated to the 100th anniversary of the Department of Otorhinolaryngology of Burdenko Voronezh State Medical University.

[Dynamics of free-radical oxidation in suppurative otitis media].

The dynamics of indicators of free-radical oxidation in various purulent-inflammatory diseases of the middle ear in 280 patients was studied. Significant violations of the processes of free radical oxidation in the serum of blood were taken for the patients, the degree of which depends entirely on the severity and nature of the inflammation. A complex treatment with the use of antioxidant therapy in 280 patients was carried out.

[The role of a leukotriene receptor antagonist in the restoration of the nasal breathing in the patients presenting with polypous rhinosinusitis].

The present study was designed to investigate the clinical. laboratory, and morphological characteristics of the nasal obstruction process. It included 87 patients presenting with polypous rhinosinusitis.

[The immune-enzyme testing of titers of serum anti-botulin antibodies type A during immunization of patients with botulin trianiatoxin].

The article discusses the effectiveness of the technique of immune-enzyme detection of anti-botulin antibodies type A in human blood serum. The presence of antibodies is registered in blood serum after second and subsequent immunization with botulin trianatoxin (titer from 1:400 to 1:3200). The establishment of relationship between the registered titers of anti-botulin antibodies and the activity of serum during protection of white mice from toxin revealed the correlation coefficient between these values as 0.

[The prooxidant system. Approaches to the choice of research algorithm].

The regulatory function of the processes involving free radicals known to maintain cellular vital activity and their role as an intermediate stage of many vitally important enzymatic reactions attract the attention of researchers interested in the mechanisms of production and inactivation of free radicals. Investigations into prooxidant and antioxidant systems along with evaluation of the intensity of free-radical oxidation make it possible to resolve many problems including elucidation of dynamic activity of pathological process, prognosis of various pathological conditions, and the choice of possible methods for the correction of prooxidant/antioxidant balance in patients with ENT diseases. The development of algorithms and methods for the assessment of parameters of activity of the prooxidant system allows for their stage by stage measurement and evaluation.

[Effect of perfluoroorganic compounds on growth of streptomycetes and biosynthesis of antibiotic daunorubicin].

The use of perfluoroorganic compounds, i.e. perfluorodecalin and "Perftoran", a blood substitute with the gas transfer function, known as "blue blood" (containing 3 vol.

Inactivation of Ebola virus with a surfactant nanoemulsion.

Hemorrhagic fever caused by Ebola virus (EBO) is a highly contagious infection. This necessitates that the contaminated instruments, clothes, and hospital premises must be completely disinfected. Nanoemulsions are a new form of disinfectant composed of detergents and vegetable oil suspended in water.

[Titration of Ebola and Marburg viruses by plaque formation under semi liquid agar].

The method of titration of Ebola and Marburg viruses using plaque formation under semifluid agar cover is considered. Advantages of this method over conventional method of titration of these viruses with the use of hard agar cover are discussed.

[Effects of hyaluronic acid preparation on the development of herpetic infection in cell culture].

Cell culture experiments demonstrated antiviral activity of a new hyaluronic acid preparation towards HSV-2. The active concentration of hyaluronic acid is at least 5%.

[Assay for level of Marburg virus in blood and isolates from experimentally infected animals].

Measurements of concentrations of Marburg virus in guinea pig saliva, urine, and feces showed that as early as by the end of incubation period the virus concentrations in the feces and saliva were 2.3-3.3 lg LD50.

[Extrachromosomal genetic elements of Clostridium botulinum. II. Isolation and analysis of DNA from bacteriophages of Clostridium botulinum types C and A].

The DNAs of bacteriophage c-st, known to realize the lysogenic conversion of toxinogenicity among C. botulinum types C and D strains, and the nucleic acid of a virulent mutant of bacteriophage CB propagated in type A C. botulinum cells were purified and examined.

[Methods for controlling colonization of air and laboratory surfaces by pathogens of certain especially dangerous viral infections].

Regular check-ups of the laboratory environment (air and working surfaces) for contamination with the objects of investigations are obligatory for laboratories working with viruses causing grave diseases, such as Ebola, Marburg, and Machupo fevers and Venezuelan equine encephalomyelitis. Methods for indication and identification of these agents have been developed and experimentally tried.

[Study of the phagocytic ability of blood polymorphonuclear leukocytes from rabbits and guinea pigs upon administering Ebola virus].

Study of the phagocytic activity of polymorphonuclear leukocytes (PMNL) of rabbits resistant to Ebola virus and guinea pigs susceptible to it, repeatedly challenged with live or inactivated Ebola virus in accordance with the immunization protocols, showed a much higher phagocytic activity in animals resistant to the virus than in those susceptible to it. Such behavior of PMNL in guinea pigs may be explained by the absence of the necessary cytokine background activating the neutrophils.

[Effects of pathology of the gastrointestinal system on the state of the mucous membrane of the larynx and pharynx].

The hypophysis on contribution of gastrointestinal pathology to development of chronic pharyngo-laryngitis (CPL) was investigated in the study of 110 patients with catarrhal, hypertrophic, atrophic and mixed CPL (20, 12.7, 27.3, 40% of patients, respectively).

[Growth of organ cultures of mouse liver infected with Coxsackie A13 virus].

Peculiarities attending the growth and proliferation of the organ cultures of the liver of mongrel albino mice infected once with Coxsackie A-13 virus were investigated. A marked zone of growth, mostly of the epithelial cells, was determined rather early in the liver explants of mice in the experimental group, whereas in control group of mice the cell growth around the explant of the liver was either absent or very weak. Besides, a great number of lymphocytes evenly arranged in the zone of hepatocytes growth was observed in the preparations of the experimental mice liver.