Screening and identification of nanobody against inhibin α-subunit from a phage display library.

Background: Inhibin is a member of the transforming growth factor family that influences reproduction in animals.

Objective: The purpose of this study was to obtain nanobodies from the phage antibody library constructed by us that can specifically bind to inhibin α-subunit.

Methods: In this study, camels were immunized with Kazakh sheep inhibin-α protein that expressed in BL21 , and the camel VHH nanobody phage display library was prepared using nested PCR.