Cytogenetic landscape in 1012 newly diagnosed chronic lymphocytic leukemia.

Background: Chronic lymphocytic leukemia (CLL) stratification mainly relies on FISH markers according to Döhner's hierarchical model which includes high-risk FISH markers, intermediate FISH, or low-risk FISH. Recently, complex karyotype (CK) has been demonstrated as an independent negative prognostic factor in CLL.

Methods: A series of 1012 untreated CLL patients have been investigated with both FISH and chromosome banding analysis (CBA) on the same pellet obtained from interleukin IL-2-CPG DSP30 oligonucleotide-stimulated cultured cells.

Onsets of progression and second treatment determine survival of patients with symptomatic Waldenström macroglobulinemia.

Few reports assess prognosis during follow-up of patients with symptomatic Waldenström macroglobulinemia (WM). In 121 WM patients treated between 1993 and 2016, we analyzed the prognostic role during the clinical course of the initial International Prognostic Scoring System for WM (IPSSWM). Then, we assessed onset of response, progression, and second treatment initiation coded as time-dependent covariates.

Lenalidomide is safe and active in Waldenström macroglobulinemia.

Lenalidomide is manageable and effective in multiple myeloma, particularly in elderly patients. Surprisingly, the combination of lenalidomide with rituximab produced clinically significant anemia at 25 mg/day for 21/28 days, the highest possible dose, in Waldenström's Macroglobulinemia (WM). We aimed to determine the maximum tolerated dose (MTD) of single agent lenalidomide and determine its impact on WM.

Sentinel node biopsy and neoadjuvant chemotherapy in the treatment of breast cancer.

Purpose: Sentinel lymph node biopsy (SLNB) has become a safe and accurate alternative to axillary lymph node dissection (ALND) in the surgical management of early breast cancer. The aim of this study was to determine the false negative rate of SLNB in patients with advanced breast cancer after neoadjuvant chemotherapy.

Methods: Forty-eight patients with 49 advanced breast cancers (one patient had bilateral disease) underwent neoadjuvant chemotherapy.

[Lymphatic mapping and biopsy of sentinel lymph nodes using combined methodology of in vivo application of Patentblue and radionuclide and ex vivo detection of metastatic affection of lymph nodes in colorectal carcinoma].

Aim Of The Study: to check the new technique of lymphatic mapping and sentinel node biopsy by colorectal cancer surgery and to improve the lymphatic staging.

Method: combined technique of lymphatic mapping via Patentblue and the radiocolloid in vivo applied in the rectal cancer surgery. The lymphatic-mapping technique with Patentblue in the colon cancer surgery.

Fully automated 3D basal ganglia activity measurement in dopamine transporter scintigraphy (Spectalyzer).

Objectives: Semiquantitative evaluation of tracer uptake in basal ganglia is superior to visual assessment of images in dopamine transporter (DAT) scintigraphy especially in follow-up of the patients. Manual drawing of regions of interest (ROIs) in two-dimensional (2D) transaxial slices of the single photon emission computed tomography (SPECT) datasets leads to a large inter- and intra-reader variability, while being time consuming. Our aim was to investigate a technique that extracts 3D ROIs in a fully automated fashion and thus might provide reproducible user-independent results allowing better follow-up control and large-scale clinical studies.

[Sentinel lymph node biopsy in the breast carcinoma in clinical practice].

Aim: In the management of early breast carcinoma, biopsy of sentinel lymph nodes has gradually replaced dissection of Level I and II axillary nodes. The aim of the study is to assess feasability and reliability of the method in our conditions.

Method: From June 1998 to June 2007, a total of 458 sentinel node biopsies (SLNB) were performed.

Prevalence and determinants of delayed gastric emptying in hospitalised Type 2 diabetic patients.

Aim: To determine the prevalence of delayed gastric emptying (GE) in older patients with Type 2 diabetes mellitus.

Methods: One hundred and forty seven patients with Type 2 diabetes, of whom 140 had been hospitalised, mean age 62.3 +/- 8.

[Distribution of metastatic affection in colorectal carcinoma using lymphatic mapping and radiation-navigated biopsy of the sentinel lymph node].

Aim: The aim is to define distribution of the lymphonode metastatic affection in colorectal carcinoma and to evaluate a new methodology of lymphatic mapping and the sentinel lymphonode detection during colorectal carcinoma procedures in practice. USED METHODS: A method of peroperative lymphatic mapping using a Patentblue method in vivo. Rectoscopic peritumoral application of a radiocoloid in a two-day or a single-day protocol, scintigraphy, peroperative quants of radioactivity detection using a gamma probe.

Transient ischemic dilation ratio (TID) correlates with HbA(1c) in patients with diabetes type 2 with proven myocardial ischemia according to exercise myocardial SPECT.

Objective: Abnormal values of the transient ischemic dilation ratio (TID) according to an exercise myocardial SPECT are linked to severe coronary artery disease. The authors investigated the relationship between TID and the levels of VCAM, ICAM, E-selectin, microalbuminuria, intima-media thickness and HbA(1c) of diabetic subjects.

Methods: We observed 38 subjects with diabetes type 2 (10 women, 28 men), of average age 56.

[New aspects of diabetes].

Type II diabetes is a serious, insidious disease which is growing at an impressive rate, with 200 million diabetics worldwide and as many who ignore their state. Having been seriously studied over more than a century and a half, an enormous quantity of knowledge regarding this disease has been accumulated. The research we are conducting has allowed us to identify the most important actors responsible for diabetes.

[Surgical therapy of the ductal carcinoma in situ].

Aim: Rates of the newly-detected DCIS reach up to 20% in developed countries. There is no unified therapeutic scheme to deal with the disorder. The aim of this work is to assess the author's own therapeutic results.

[Mini-invasive radiation-navigated parathyreoidectomy--MIRP].

Aim: The aim was to introduce a new surgical method and to verify its validity.

Methodology: In 20 patients whose ultrasound findings correlated with the MIBI scintigraphic results, a radiation-navigated parathyreoidectomy using a C-Track was conducted, following a radionucleotide application.

Results: From 2001 to 2003, 20 patients with hyperparathyreosis were operated.

[Lymphadenectomy in the early gastric carcinoma].

Aim: The method of extended lymphadenectomy in the early gastric carcinoma treatment remains controversial. The aim of this prospective study is to assess the above method feasibility with acceptable rates of complications, in our conditions.

Methodology: From 2000 to 2003, 11 patients with early carcinomas of the stomach were treated using the method of extended lymphadenectomy.

[Sentinel lymph node biosy in surgical treatment of breast carcinoma: prospective study].

Objective: Authors report the validity and accuracy of lymphatic mapping with sentinel node biopsy in patients with early breast cancer between 1998 and 2000.

Type Of Study: Prospective study.

Location: Department of Surgery, Atlas Hospital Zlin.

Pulsed Doppler tissue imaging of the velocity of tricuspid annular systolic motion; a new, rapid, and non-invasive method of evaluating right ventricular systolic function.

Aims: Rapid, accurate, and widely available non-invasive evaluation of right ventricular function still presents a problem. The purpose of the study was to determine whether the parameters derived from Doppler tissue imaging of tricuspid annular motion could be used as indexes of right ventricular function in patients with heart failure.

Methods: Standard and pulsed Doppler tissue echocardiography were obtained in 44 patients with heart failure (mean left ventricular ejection fraction 24 +/- 7%) and in 30 age- and sex-matched healthy volunteers.

Involvement of thymosin beta 4 and endoproteinase Asp-N in the biosynthesis of the tetrapeptide AcSerAspLysPro a regulator of the hematopoietic system.

It is shown that AcSDKP a new regulator of the hematopoietic system can be generated from thymosin beta 4 by a one-step enzymatic cleavage in vitro and in vivo. AcSDKP and T beta 4 were both detected in bone marrow cells (BMC). Incubation of [3H]T beta 4 with either intact or lysed BMC led to the formation of [3H]AcSDKP whereas the labelled tetrapeptide was not degraded under these conditions.

Intravenous radionuclide angiography after unilateral hypogastric artery ligation. Suture material and recanalization.

Pelvic angioscintigraphic examination of seven patients after unilateral hypogastric artery ligation has been performed. Immediately after the operation asymmetric perfusion has been observed in all women. Subsequent control examination of 4 patients ligatured by nonabsorbable suture has shown persisting unilateral hypoperfusion.