A mechanism for the hypoprothrombinemia of the acquired hypoprothrombinemia-lupus anticoagulant syndrome.

Antibodies that bind prothrombin without neutralizing its coagulant activity were demonstrated in the plasma of two patients with the acquired hypoprothrombinemia-lupus anticoagulant syndrome. The first patient's plasma contained less than 1% prothrombin activity and no detectable prothrombin antigen. The second patient's plasma contained about 6% of both prothrombin activity and antigen.

Studies on the composition, quality and processing of triticale. Part II. Mineral composition of grain and flour.

Mineral composition of grain and flour in 14 promising triticale strains from India are reported. The mean values for whole grain, i.e.

Studies on the composition, quality and processing of triticale. Part I. Physico-chemical characteristics.

Studies on the physico-chemical characteristics of 14 promising strains of triticale and two check wheat varieties (Kalyansona and WL 711) grown under similar agronomic conditions were conducted. Significant differences were obtained for 1000 kernel weight ranging from 34.9 to 51.

A procedure for isolation of human protein C and protein S as by-products of the purification of factors VII, IX, X and prothrombin.

A DEAE-Sephadex column chromatography step utilized to purify human Factor VII consistently yields a protein peak between the factor VII activity peak and prothrombin, factor X and factor IX activity peak (S.P. Bajaj, S.

Contact Hypersensitivity to Topical Antimicrobial and Antifungal Agents.

To find out the incidence of hypersensitivity to antimicrobial drugs patch tests with various commercially available antimicrobial agents were carried out in 112 patients suspected of contact sensitivity to these substances. Eighty eight patients showed positive reaction to one or more drugs. H incidence of contact sensitivity was observed with neomycin (48%), nitrofurazone (460/o) and bromsalicyl chloranilide (45%), while gentian violet gentamicin and povidone iodine were found to be the least sensitizers (5%, 6% and 70/o - respectively).

Activation of human factor VII by activated factors IX and X.

Factor VII clotting activity increases about five-fold when blood is clotted in glass. Prior studies suggested that this results from activation induced by activated factor IX (IXa). However, in purified systems containing phospholipid and calcium, activated factor X (Xa) is known to activate factor VII rapidly.

Tissue factor-like activity of the human monocytic tumor cell line U937.

Undisrupted cells of the human monocytic tumor cell line U937 have procoagulant activity that is Ca2+ dependent and is not demonstrable in Factor VII or Factor X deficient plasma. Furthermore, U937 cells when incubated with purified human Factor VII in the presence of Ca2+ and then repeatedly washed promoted coagulation of Factor VII deficient plasma in the absence of added tissue factor. Culture with endotoxin increased the procoagulant activity of U937 cells approximately 5-fold.

Heterogeneity in human prothrombin: analysis of cause.

Two fractions of human prothrombin can be isolated from single donor plasma by the technique of heparin-agarose chromatography in (sodium) citrate buffer, pH 7.5, as previously reported for pooled plasma. The two fractions, designated H-II1 and H-II2, are found in a ratio of approximately 4:1.

Isolation and characterization of human factor VII. Activation of factor VII by factor Xa.

A procedure has been developed for the isolation of human Factor VII to apparent homogeneity as judged by the analytical disc electrophoretic system of Davis (pH 8.9) and by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The isolated procedure involves adsorption of Factor VII onto barium citrate, ammonium sulfate fractionation, DEAE-Sephadex chromatography, and preparative polyacrylamide gel electrophoresis.

A simplified procedure for purification of human prothrombin, factor IX and factor X.

A simplified procedure is described for the purification of prothrombin, Factor X and Factor IX in overall yields of 35-40% from pooled human plasma. The initial steps, which are common to prior purification techniques, include adsorption onto and elution from barium citrate, ammonium sulfate fractionation, and DEAE-Sephadex chromatography. The procedure differs from previous techniques in that the nest step, heparin-agarose chromatography, is carried out in a (sodium) citrate buffer, pH 7.

Properties of gastric antrum. III. Selectivity and modification of shunt conductance.

The permselectivity of the paracellular pathway of amphibian (Necturus and bullfrog) antrum was investigated with respect to intracationic selectivity and the K+ and Cl- permeability ratio as a function of mucosal pH. The intracationic selectivity sequence was Rb+ greater than K+ greater than Cs+ greater than Na+ greater than Li+. Both antra showed weak cationic selectivity at pH 7.

Interaction of manganese with bovine prothrombin and its thrombin-mediated cleavage products.

The binding of the paramagnetic metal, Mn(II), to bovine prothrombin and the thrombin-mediated cleavage products of prothrombin, i.e. fragment 1 and the prethrombin 1 has been investigated.

Human plasma lipoproteins as accelerators of prothrombin activation.

The activation rate of bovine prothrombin by Factor Xa and Ca2+ has long been known to be greatly enhanced by addition of phospholipid. Upon substitution of human plasma lipoproteins for phospholipid (cephalin) in this activation system, only very low density lipoprotein enhances prothrombin activation. Low density lipoprotein and high density lipoprotein have no stimulatory effect on prothrombin activation.

Inhibition of basal and stimulated gastric H+ and pepsin secretion in duodenal ulcer patients by metiamide, an H-2 histamine antagonist.

Metiamide was given orally in one dose of 200 mg in 23 sutdies in patients with duodenal ulcer, 4 in the basal state, 11 during histamine infusion, and 8 before insulin hypoglycemia stimulation. In the latter 8 patients insulin was given at another time without metiamide. In 17 studies acid secretion was suppressed by metiamide--up to 75% in the basal state, 53% after histamine, and 80% after insulin.

Gastric acid secretory responses to graded intravenous doses of histamine in hookworm anemia.

Dose-response curves for the effect of continuous infusion of histamine on gastric acid secretion were obtained in 10 patients with iron deficiency anemia due to hookworm infection. Each patient was studied with 4 doses (40, 60, 80 and 100 mug/kg/h) of histamine acid phosphate administered for 3 h, on separate days, in random fashion. The acid output increased with the dose of histamine, the maximal acid output was reached in all patients at the dose of 100 mug/kg/h.

Prothrombin fragments. Ca2+ binding and activation kinetics.

The binding of Ca2+ to prothrombin and the intermediates of prothrombin activation was investigated by equilibrium dialysis using 45Ca2+ as the ligand. Scatchard plots of these data indicate that prothrombin (Mr = 70,000) has 10 to 11 Ca2+ binding sites which can be differentiated in terms of their binding affinity. Six of these Ca2+ binding sites have log Kassoc = 3.