Comparative physiological and co-expression network analysis reveals potential hub genes and adaptive mechanisms responsive to NaCl stress in peanut (Arachis hypogaea L.).

Background: Salt stress has become a major threat to peanut yield and quality, and salt stress is particularly detrimental to seedling growth. Combined analysis of the physiology and transcriptomics of salt-tolerant variety (NH5) and salt-sensitive variety (FH23) under 200 mM NaCl stress was conducted to identify the key factors influencing the differences in salt tolerance and to investigate the potential regulatory mechanisms and hub genes associated with salt tolerance in peanuts.

Results: Malondialdehyde (MDA) content and electrolyte leakage rate were significantly increased under prolonged NaCl stress, with the increase in FH23 being even more pronounced.

Integrative multi-omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings.

Plant growth is restricted by salt stress, which is a significant abiotic factor, particularly during the seedling stage. The aim of this study was to investigate the mechanisms underlying peanut adaptation to salt stress by transcriptomic and metabolomic analysis during the seedling stage. In this study, phenotypic variations of FH23 and NH5, two peanut varieties with contrasting tolerance to salt, changed obviously, with the strongest differences observed at 24 h.

Identification of candidate genes associated with peanut pod length by combined analysis of QTL-seq and RNA-seq.

Pod length (PL) is one of the major traits determining pod size and yield of peanut. Discovering the quantitative trait loci (QTL) and identifying candidate genes associated with PL are essential for breeding high-yield peanut. In this study, quantitative trait loci sequencing (QTL-seq) was performed using the F population constructed by a short-pod variety Tifrunner (Tif) and a long-pod line Lps, and a 0.