Publications by authors named "Bailey B Banach"
Article Synopsis
- * Yeast cells exhibit a wider size range compared to mammalian cells, which makes it challenging to accurately determine single-cell gates during FACS.
- * The report investigates different gating options for yeast display and proposes an optimized flow cytometry method to enhance the selection of single yeast cells, leading to improved results in yeast display studies.
The HIV-1 fusion peptide (FP) represents a promising vaccine target, but global FP sequence diversity among circulating strains has limited anti-FP antibodies to ~60% neutralization breadth. Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display.
View Article and Find Full Text PDFThe monoclonal antibody CIS43 targets the Plasmodium falciparum circumsporozoite protein (PfCSP) and prevents malaria infection in humans for up to 9 mo following a single intravenous administration. To enhance the potency and clinical utility of CIS43, we used iterative site-saturation mutagenesis and DNA shuffling to screen precise gene-variant yeast display libraries for improved PfCSP antigen recognition. We identified several mutations that improved recognition, predominately in framework regions, and combined these to produce a panel of antibody variants.
View Article and Find Full Text PDFArticle Synopsis
- Antiviral monoclonal antibody (mAb) discovery aims to develop effective treatments for viral infections, but traditional methods are often inefficient due to the high affinity of antibodies not always translating to neutralizing activity.
- Researchers explored screening for anti-SARS-CoV-2 mAbs at lower pH levels, specifically pH 4.5, and found that neutralizing antibodies were more effectively enriched at this acidity compared to physiological pH (7.4).
- A new antibody, LP5, was identified that targets a key area of the SARS-CoV-2 virus, demonstrating the potential of low pH screening to enhance the discovery of effective antiviral antibodies.
Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a public antibody response encoded by IGHV3-53/IGHV3-66 genes. Sequence and structural analyses of 910-30 and related antibodies explore how class recognition features correlate with SARS-CoV-2 neutralization.
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- A new antibody, 910-30, has been identified that targets the SARS-CoV-2 ACE2 receptor binding site, part of a public antibody response encoded by specific IGHV genes.
- Structural analyses via Cryo-EM show how 910-30 interacts with the SARS-CoV-2 spike trimer, revealing its ability to bind and disassemble the spike protein.
- The study highlights important sequence and structural signatures for neutralization in the IGHV3-53/3-66 antibody class, with a notable prevalence of antibody precursors found in healthy human B cells.
Biotin-labeled molecular probes, comprising specific regions of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike, would be helpful in the isolation and characterization of antibodies targeting this recently emerged pathogen. Here, we design constructs incorporating an N-terminal purification tag, a site-specific protease-cleavage site, the probe region of interest, and a C-terminal sequence targeted by biotin ligase. Probe regions include full-length spike ectodomain as well as various subregions, and we also design mutants that eliminate recognition of the angiotensin-converting enzyme 2 (ACE2) receptor.
View Article and Find Full Text PDFArticle Synopsis
- - Researchers developed biotin-labeled molecular probes from regions of the SARS-CoV-2 spike protein to help isolate and study antibodies against the virus.
- - The design involved using a purification tag and a targeted protease-cleavage site, yielding different amounts of biotin-labeled probes, with specific structures confirmed through cryo-electron microscopy.
- - Funding for the project came from multiple sources, including the National Institute of Allergy and Infectious Diseases and various grants, while no conflicts of interest were reported by the authors.
Article Synopsis
- Researchers created biotin-labeled molecular probes targeting specific regions of the SARS-CoV-2 spike protein to help isolate and study antibodies against the virus.
- These probes included various designs, such as full-length spike regions and modifications to prevent ACE2 receptor recognition, yielding different amounts during production.
- The resulting probes were tested for their ability to bind antibodies and were structurally analyzed using advanced imaging techniques, showcasing the effectiveness of combining structural design with purification methods.
Juvenile neuronal ceroid lipofuscinosis (JNCL) is a childhood neurodegenerative disease with early-onset, severe central vision loss. Affected children develop seizures and CNS degeneration accompanied by severe motor and cognitive deficits. There is no cure for JNCL, and patients usually die during the second or third decade of life.
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