Amphotericin B and Curcumin Co-Loaded Porous Microparticles as a Sustained Release System against .

Amphotericin B (AMB) is an antifungal drug used for serious fungal infections. However, AMB has adverse reactions such as nephrotoxicity, which limit the clinical application of AMB alone or in combination with other antifungal drugs. Nano or micro drug delivery systems (DDS) have been proven to be effective in reducing the toxic and side effects of drugs.

Fungus-mediated green synthesis of nano-silver using Aspergillus sydowii and its antifungal/antiproliferative activities.

Due to the increasing demand for eco-friendly, cost-effective and safe technologies, biosynthetic metal nanoparticles have attracted worldwide attention. In this study, silver nanoparticles (AgNPs) were extracellularly biosynthesized using the culture supernatants of Aspergillus sydowii. During synthesis, color change was preliminarily judge of the generation of AgNPs, and the UV absorption peak at 420 nm further confirms the production of AgNPs.

Micronized curcumin fabricated by supercritical CO to improve antibacterial activity against .

Curcumin (CM) is a natural polyphenolic compound with multiple biomedical functions. However, clinical applications face more challenges due to its low dissolution rate and poor bioavailability. Micronization is an effective strategy to overcome these drawbacks.

Curcumin-Silk Fibroin Nanoparticles for Enhanced Anti- Activity and .

Curcumin (CM) has multiple pharmacological activities including anti-fungal activity, but its clinical application is limited due to low solubility in aqueous media, poor bioavailability and extensive first pass metabolism. We aimed to resolve the limitation and enhance antifungal activity of CM using nanotechnology. Using silk fibroin (SF) as a carrier, we fabricated curcumin-silk fibroin nanoparticles (CM-SF NPs) by solution enhanced dispersion of supercritical CO₂ (SEDS) technique.

Reducing the toxicity of amphotericin B by encapsulation using methoxy poly(ethylene glycol)-b-poly(l-glutamic acid-co-l-phenylalanine).

Amphotericin B (AmB) is an antifungal drug used for serious fungal infections and leishmaniosis. However, its clinical application is limited because of its high toxicity. To resolve this problem, herein we loaded AmB into methoxy poly(ethylene glycol)-b-poly(l-glutamic acid-co-l-phenylalanine) (mPEG-b-P(Glu-co-Phe)) nanoparticles (l-AmB) via electrostatic, hydrophobic and π-π interactions.

Deletion of afpab1 Causes Increased Sensitivity to Oxidative Stress and Hypovirulence in Aspergillus fumigatus.

AFPAB1 is the ortholog of the cytoplasmic messenger ribonucleoprotein granules AOPAB1 that function to depress the initiation of translation during stress. can regulate its cellular physiology in response to environmental stresses, but there has been no research on Pab1 in . The associated gene was replaced with a hygromycin-selectable marker to generate the strain .

Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium.

The objective of this study was to find one or more fungal strains that could be utilized to biosynthesize antifungal silver nanoparticles (AgNPs). Using morphological and molecular methods, Arthroderma fulvum was identified as the most effective fungal strain for synthesizing AgNPs. The UV-visible range showed a single peak at 420 nm, which corresponded to the surface plasmon absorbance of AgNPs.

Identification and characterization of an anti-oxidative stress-associated mutant of Aspergillus fumigatus transformed by Agrobacterium tumefaciens.

Aspergillus fumigatus is one of the most common opportunistic pathogenic fungi, surviving in various environmental conditions. Maintenance of the redox homeostasis of the fungus relies upon the well‑organized regulation between reactive oxygen species generated by immune cells or its own organelles, and the activated anti‑oxidative stress mechanism. To investigate such a mechanism, the present study obtained a number of randomly‑inserted mutants of A.