Publications by authors named "Baihan Gao"
Article Synopsis
- Plant secondary metabolites, like those from Astragalus membranaceus, are important for new drug development but are hard to extract due to their complex structures and low quantities.
- The study focused on glycosyltransferases (GTs) from A. membranaceus to create diverse astragalosides, leading to the identification of new GTs that can synthesize various sugar substitutions efficiently.
- The research established a combinatorial synthesis system that produced 13 new astragalosides with significant improvements in their biological activity, showcasing a novel approach to enhance herbal compounds for medical use.
Engineering the function of triterpene glucosyltransferases (GTs) is challenging due to the large size of the sugar acceptors. In this work, we identified a multifunctional glycosyltransferase AmGT8 catalyzing triterpene 3-/6-/2'-O-glycosylation from the medicinal plant Astragalus membranaceus. To engineer its regiospecificity, a small mutant library was built based on semi-rational design.
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