Publications by authors named "Baifeng Zhou"
Article Synopsis
- Nitrogen assimilation is crucial for plant growth, with transcription factors (TFs) playing a key role in adjusting gene expression based on nitrogen availability.
- Researchers discovered a negative domain (ND) in the CmMYB1 TF from a unicellular red alga, which affects its localization and function in nitrogen-rich conditions.
- The study identified the CmNDB1 protein, which interacts with ND, and both ND and CmNDB1 were found to negatively regulate CmMYB1, impacting the expression of nitrate assimilation genes when nitrogen is abundant.
Microalgal triacylglycerols (TAGs) are a good feedstock for liquid biofuel production. Improving the expression and/or function of transcription factors (TFs) involved in TAG accumulation may increase TAG content; however, information on microalgae is still lacking. In this study, 14 TFs in the unicellular red alga were identified as candidate TFs regulating TAG accumulation using available transcriptome and phosphoproteome data under conditions driving TAG accumulation.
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- The firefly luciferase (Luc) reporter assay is used to study promoter activities in living cells, and this report focuses on establishing it in the red alga Cyanidioschyzon merolae.
- In this study, a NIR promoter-Luc fusion gene was added to the C. merolae genome, showing increased luciferase activity as endogenous NIR transcripts rose due to nitrogen depletion.
- Additionally, overexpressing the MYB1 gene, which activates the NIR promoter, further boosted Luc activity, showcasing the effectiveness of the Luc reporter system in this algal model.