Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
October 2010
Aim: to detect the expression of Hedgehog pathway components PTCH-1 and SMO mRNA in nasopharyngeal carcinoma (NPC), and discuss its biological significance.
Methods: expression of PTCH-1 and SMO mRNA was evaluated by real-time fluorescence quantitative PCR in 32 cases of NPC and 32 cases of nasopharyngitis. According to Livak (2(-delta;Ct);) relative quantitative analysis method, with nasopharyngitis as control, to calculate the expression level of PTCH-1 and SMO in NPC.