Publications by authors named "Bai-Hui Xu"
Molecular mechanism of di-n-butyl phthalate promotion of bladder cancer development.
Toxicol In Vitro
February 2023
Purpose: To determine whether di-n-butyl phthalate (DBP) promotes the occurrence of bladder cancer (BCa) and explore the action of DBP acts on BCa cells at the cellular and molecular levels.
Methods: MTT and Transwell assays were used to investigate the tumorigenic actions of DBP on BCa cells. Second-generation sequencing was used to identify differences in gene expression before and after DBP treatment.
Article Synopsis
- Graphene nanomaterials, particularly graphene quantum dots (GQDs), show promise in biomedical applications due to their exceptional properties, but concerns exist regarding their potential adverse effects on biological systems.
- In vitro studies revealed that high dosages of GQDs hindered the maturation of mouse oocytes, causing issues like decreased polar body extrusion rates and damage to mitochondrial structures, likely due to DNA damage and reactive oxygen species accumulation.
- In vivo experiments indicated that while high GQD exposure during pregnancy affected fetal growth, it did not impact the long-term health or reproductive success of subsequent generations, suggesting a need for continued research on GQDs for safe medical use.
Di(n-butyl) phthalate (DBP) is extensively used in industrial applications as plasticizer and stabilizer and its presence in the environment may present health risks for human. Previous studies have demonstrated its mutagenic, teratogenic, and carcinogenic ability. However, its effect on mammalian oocyte maturation remains unknown.
View Article and Find Full Text PDFSKAP2 (Src kinase-associated phosphoprotein 2), a substrate of Src family kinases, has been suggested to be involved in actin-mediated cellular processes. However, little is known about its role in mouse oocyte maturation. In this study, we thus investigated the expression, localization, and functions of SKAP2 during mouse oocyte asymmetric division.
View Article and Find Full Text PDFArticle Synopsis
- - KIF2A, a kinesin-13 family protein, is important for spindle assembly during meiotic division in mouse oocytes, but its specific role was previously unclear.
- - The study showed that KIF2A is expressed at crucial stages of meiosis, localizing in the germinal vesicle and spindle, and co-localizes with α-tubulin when spindle assembly occurs.
- - Depletion of KIF2A leads to severe issues in spindle assembly and chromosome alignment, ultimately causing problems in cytokinesis and a reduced rate of polar body extrusion, suggesting its critical role in meiosis regulation.
Methoxychlor (MXC) is used worldwide against insects and other pests. This organochlorine pesticide acts as a xenoestrogen, promotes oxidative stress, and is considered cytotoxic and genotoxic, causing abortions and stillbirths in females. Mechanistically related estrogens and oxidants affect oocyte meiosis, so we investigated the effects of MXC on mouse oocyte meiotic maturation.
View Article and Find Full Text PDFAxin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown. Here we examined the localization and function of Axin-1 during meiotic maturation in mouse oocytes. Immunofluorescence analysis showed that Axin-1 was localized around the spindle.
View Article and Find Full Text PDFA total of 1116 middle-aged and elderly men and 1442 postmenopausal women were recruited in this study. Whether bisphenol A exposure was associated with circulating sex hormone concentrations was studied. Univariate analysis revealed that the urinary bisphenol A concentration was negatively correlated with the serum levels of luteinizing hormone (β=-0.
View Article and Find Full Text PDFObjective: To study the relation between serum calcium level and elevated BaPWV in Chinese subjects.
Methods: The relation between serum calcium level and elevated BaPWV was studied in 9 615 subjects. The mean value of left and right BaPWV was analyzed.
Methylglyoxal, a reactive dicarbonyl compound, is mainly formed from glycolysis. Methylglyoxal can lead to the dysfunction of mitochondria, the depletion of cellular anti-oxidation enzymes and the formation of advanced glycation ends. Previous studies showed that the accumulation of methylglyoxal and advanced glycation ends can impair the oocyte maturation and reduce the oocyte quality in aged and diabetic females.
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