Explanted Fish Neural Tubes Give Rise to Differentiating Neural Crest Cells: (neural crest cell culture/pigment cells/Xiphophorus/medaka/fish).

Neural tubes were explanted from the trunk of various embryonic stages of three teleost fish, Xiphophorus maculatus (platyfish), X. helleri (swordtail), and Oryzias latipes (Japanese medaka) with the aim to obtain in vitro differentiating neural crest cells. Outgrowth of cells was observed immediately after attachment of the explants on dishes coated with fibronectin.