Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran.

Introduction: Tuberculosis (TB) remains a serious public health problem worldwide. Drug-resistant TB is considered a major and growing global threat. Despite the great variety of described mutations in Mycobacterium tuberculosis (MTB) resistance genes, the mechanisms of drug resistance are still controversial.

The anticancer and antibacterial potential of bioactive secondary metabolites derived From bacterial endophytes in association with Artemisia absinthium.

The continuous search for secondary metabolites in microorganisms isolated from untapped reservoirs is an effective prospective approach to drug discovery. In this study, an in-depth analysis was conducted to investigate the diversity of culturable bacterial endophytes present in the medicinal plant A. absinthium, as well as the antibacterial and anticancer potential of their bioactive secondary metabolites.

Epstein-barr virus/ coinfection and gastric cancer: the possible role of viral gene expression and shp1 methylation.

Background And Objectives: Among the various factors involved in the development of gastric cancer (GC), infectious agents are one of the most important causative inducers. This study aimed to investigate the possible role of EBV gene expression on SHP1 methylation in co-infection with in patients with GC.

Materials And Methods: Formalin-fixed paraffin-embedded samples were obtained from 150 patients with gastrointestinal disorders.

EPIYA Motif Genetic Characterization from Isolates in Distinct Geographical Regions of Iran.

Background: This study aimed to determine the current EPIYA motifs of the A gene in isolates from patients with gastric disorders, and evaluate the association between these patterns and the clinical outcome of infection in different geographical regions of Iran.

Materials And Methods: We examined 150 patients with gastrointestinal disorders from the central and eastern regions of Iran. The detection of and screening of A was performed by polymerase chain reaction (PCR).

Viral aetiology of acute central nervous system infections in children, Iran.

Viral infections are increasingly an important cause of central nervous system (CNS) complications. There is no comprehensive insight about CNS infections due to viral agents among Iranian children. This study aimed to investigate the viral aetiology, clinical and epidemiological profile of children with acute infections of the CNS.

A Phage Cocktail To Control Surface Colonization by Proteus mirabilis in Catheter-Associated Urinary Tract Infections.

Proteus mirabilis is a biofilm-forming bacterium and one of the most common causes of catheter-associated urinary tract infections (CAUTIs). The rapid spread of multidrug-resistant P. mirabilis represents a severe threat to management of nosocomial infections.

Alhagi maurorum extract modulates quorum sensing genes and biofilm formation in Proteus mirabilis.

Proteus mirabilis (P. mirabilis) is a frequent cause of catheter-associated urinary tract infections. This study aims to investigate the anti-infective effect of Alhagi maurorum extract (AME), the traditional medicinal plant in the middle east, on the biofilm-forming P.

Determination of Capsular Serotypes, Antibiotic Susceptibility Pattern, and Molecular Mechanism of Erythromycin Resistance among Clinical Isolates of Group B in Isfahan, Iran.

Background: Documented streptococcal resistance to erythromycin has recently been raised. The aim of this study is to identify the molecular mechanism of erythromycin resistance among group B (GBS) strains and to correlate with the clinical origin of strains.

Materials And Methods: A total number of 134 colonizing ( = 36), invasive ( = 36), noninvasive ( = 46), and asymptomatic ( = 16) GBS isolates were characterized by the detection of gene, capsular serotyping, antibiotic susceptibility profiles using disc diffusion method, and screening of the , , and resistance genes.

From the Urinary Catheter to the Prevalence of Three Classes of Integrons, -Lactamase Genes, and Differences in Antimicrobial Susceptibility of and Clonal Relatedness with Rep-PCR.

Introduction: is a biofilm-forming agent that quickly settles on the urinary catheters and causing catheter-associated urinary tract infections. Thus, the spread of multidrug-resistant isolates, with the ability to form a biofilm that carries integron, extended-spectrum -lactamases (ESBLs), and plasmid-mediated colistin resistance genes (), represents a severe threat to managing nosocomial infectious diseases. This study is aimed at surveying the prevalence of ESBL, integrase, and genes of , isolated from the catheter, to assess the differences in their antimicrobial susceptibility and clonal dissemination.

Comparison of the Prevalence of Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) among Isolates in a Burn Unit with Non-Burning Units.

Background: () is one of the most important pathogens in burn infections colonized in the nose and increase the risk of infections.

Methods: Overall, 85 isolates were isolated from clinical and nasal hospitalized patients and health care workers (HCWs) in a burn unit and non-burn units in Isfahan from June 2016 and September 2016. Genes encoding penicillin-binding protein 2a A) and adhesive surface proteins, including fibronectin-binding proteins (,), fibrinogen binding protein (), laminin-binding protein(), collagen binding protein (), elastin binding protein intracellular adhesion operon (A and D) were detected using PCR method.

Significance of the coexistence of non-codon 315 katG, inhA, and oxyR-ahpC intergenic gene mutations among isoniazid-resistant and multidrug-resistant isolates of Mycobacterium tuberculosis: a report of novel mutations.

Tuberculosis (TB) is a global threat due to the emergence and spread of drug-resistant (MTB). Isoniazid (INH) is the main antibiotic used for prevention and treatment of TB. Evidence shows that accumulated mutations can produce INH resistant (INHR) strains, resulting in the progression of multidrug-resistant (MDR) TB.

Prevalence, population structure, distribution of serotypes, pilus islands and resistance genes among erythromycin-resistant colonizing and invasive Streptococcus agalactiae isolates recovered from pregnant and non-pregnant women in Isfahan, Iran.

Background: The information on antibiotic resistance and molecular features of Group B Streptococcus (GBS) are essential for epidemiological purposes as well as vaccine development. Therefore, we aimed to assess the antimicrobial resistance profiles and molecular characteristics of GBS isolates in Isfahan, Iran. A total number of 72 colonizing and invasive GBS were collected from pregnant and non-pregnant women.

Molecular Characterization of Hospital- and Community-Acquired Isolates among Nonpregnant Adults in Isfahan, Iran.

Background: The increasing incidence of Group B Streptococcus (GBS) infection among nonpregnant adults has become of growing clinical and public health concern. The current study investigated the distribution of important virulence determinants and antibiotic susceptibility of GBS isolates causing community acquired (CA) and hospital acquired (HA) infections among nonpregnant adults.

Materials And Methods: A total of 62 GBS, including 31 CA GBS and 31 HA GBS, were collected from a teaching hospital in Isfahan, Iran.

Global Sequence Analysis and Expression of Gene in Different Clinical Specimens of Burn Patients with Infection.

Aim: The purpose of this study was to analyze the sequence of gene in relation to its expression in strains isolated from different clinical specimens of burn patients. Moreover, in silico sequence analysis of gene using globally reported sequences was intended.

Materials And Methods: Fifty-nine multidrug-resistant isolates were selected from different clinical specimens of patients suffering from burn wound infections in two university hospitals and subjected to antibacterial susceptibility testing.

Sequence-based detection of first-line and second-line drugs resistance-associated mutations in Mycobacterium tuberculosis isolates in Isfahan, Iran.

Tuberculosis is an infectious disease, which requires special medical attention due to the emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. The present study aimed to assess drug resistance to first-line anti-mycobacterial drugs, including rifampin (RIF), isoniazid (INH), and ethambutol (EMB), as well as second-line drugs, including ofloxacin (OFX), kanamycin (KAN), amikacin (AMK), and capreomycin (CAP). The following eight loci were investigated to evaluate drug resistance: rpoB, katG, inhA, and embB, associated with resistance to RIF, INH, and EMB and gyrA, rrs, eis, and tlyA, associated with resistance to OFX, AMK, KAN, and CAP.

Targeting of cholera toxin A () gene by zinc finger nuclease: pitfalls of using gene editing tools in prokaryotes.

Background And Purpose: The study was launched to use zinc finger nuclease (ZFN) technology to disrupt the cholera toxin gene () for inhibiting CT toxin production in .

Experimental Approach: An engineered ZFN was designed to target the catalytic site of the gene. The coding sequence of ZFN was cloned to pKD46, pTZ57R T/A vector, and plasmid and transformed to Top10 and .

Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 .

Herpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections.

Molecular Characterization Of Vancomycin-Resistant Among Inpatients At Iranian University Hospitals: Clonal Dissemination Of ST6 And ST422.

Purpose: Over the past two decades, enterococci have emerged as an important opportunistic pathogen causing life-threatening infections in hospitals. The purpose of the present study was to examine the prevalence of genes encoding virulence factor and molecular characterization of vancomycin-resistant strains isolated from hospitalized patients in Isfahan, the central city of Iran.

Patients And Methods: A total of 53 vancomycin-resistant isolates (VRE) obtained from clinical samples of hospitalized patients were characterized by phenotypic and genotypic methods, and 25 selected VRE isolates from internal and ICU wards were typed by multilocus sequence typing.

Analysis of antibacterial and antibiofilm activity of purified recombinant Azurin from .

Background And Objectives: The aim of this study was to evaluate the antibacterial and antibiofilm activity of recombinant Azurin from against different bacterial species.

Materials And Methods: The gene was cloned in the pET21a vector. The pET21a- construct was transformed into BL21.

Determination of surface proteins profile, capsular genotyping, and antibiotic susceptibility patterns of Group B Streptococcus isolated from urinary tract infection of Iranian patients.

Objectives: Group B Streptococcus (GBS) is an important opportunistic bacteria that causes a wide range of infections including neonatal sepsis, meningitis, pneumonia, soft tissue and urinary tract infections (UTI). The aim of this study was to evaluate the antimicrobial susceptibility patterns, surface proteins and capsular types of GBS isolates.

Results: 100 of UTI isolates were confirmed as GBS.

Phylogenetic analysis of human bocavirus in children with acute respiratory infections in Iran.

Human bocavirus (HBoV) was first characterized in nasopharyngeal aspirates from young children with acute respiratory infections. It is prevalent among children with acute wheezing. This study was carried out in order to analyze the infection frequency and coinfection rates of HBoV with respiratory syncytial virus (RSV) and to perform phylogenetic analysis of HBoV in samples of children with acute respiratory infection in Isfahan, Iran.

Phenotypic and genotypic characterization of glycopeptide, aminoglycoside and macrolide resistance among clinical isolates of Enterococcus faecalis: a multicenter based study.

Objectives: Enterococcus faecalis as part of the normal floras of human gastrointestinal and genitourinary tracts are an important cause of nosocomial infections. The present study aimed to investigate the prevalence of genes encoding antimicrobial resistance and genetic relatedness of clinical isolates of E. faecalis among Iranian hospitalized patients.