Molecular characterization of methicillin-resistant Staphylococcus aureus clones from a teaching hospital in Tehran.

A total of 52 methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from patients attending the teaching hospital of Tehran University of Medical Sciences. Disks containing antibiotics were used to determine the susceptibility of MRSA isolates. Analysis of SmaI macrorestriction profiles of the 52 MRSA isolates were grouped into three PFGE types.

Characterisation of genes encoding aminoglycoside-modifying enzymes among meticillin-resistant Staphylococcus aureus isolated from two hospitals in Tehran, Iran.

Meticillin-resistant Staphylococcus aureus (MRSA) is a major hospital pathogen and typically shows resistance to multiple antimicrobial agents. The susceptibility patterns of 109 MRSA isolates to aminoglycoside antibiotics were determined by the disk diffusion method. Genes encoding the aminoglycoside-modifying enzymes (AMEs) were targeted by polymerase chain reaction (PCR) assays.

Staphylococcal cassette chromosome mec (SCCmec) analysis and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) isolates in Tehran, Iran.

Oxacillin resistance was present in 99 of 277 (36%) consecutive Staphylococcus aureus isolates collected from hospital patients in Tehran during a 15-month period (January 2004-March 2005). The majority of isolates (77/99 = 78%) had been cultured from wounds or blood. The staphylococcal cassette chromosome mec (SCCmec) types and antimicrobial susceptibility patterns of 99 methicillin-resistant S.