Method development and validation of Guanfacine in rat plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study.

A selective, sensitive and high-throughput liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) method has been developed and validated for the quantitation of Guanfacine in rat plasma. Sample clean-up involved liquid-liquid extraction (LLE) and 100 μL of rat plasma was used. YMC BASIC column (50 mm×2.

Essential oils of Retama raetam from Libya: chemical composition and antimicrobial activity.

Retama raetam (Forssk) Webb & Berthel is well known in the folk medicine of North and East Mediterranean regions for the treatment of microbial infections. The powdered leaves are used to heal circumcision wounds and used as an antiseptic for wounds, skin rash and pruritus. In this study, to validate this antiseptic property, the chemical composition and antimicrobial activity of the essential oil from the flowers of R.

LC-ESI-MS/MS method for the quantification of entecavir in human plasma and its application to bioequivalence study.

Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) was used for a quantitative estimation of entecavir (EV) in human plasma using lamivudine (LM) as internal standard (IS). The method herein described is simple, sensitive, and specific. Chromatographic separation was performed on XBridge-C18, 4.

Method development and validation of montelukast in human plasma by HPLC coupled with ESI-MS/MS: application to a bioequivalence study.

A simple, sensitive, and specific LC-ESIâMS/MS method for quantification of Montelukast (MO) in human plasma using Montelukast-d(6) (MOD6) as an internal standard (IS) is discussed here. Chromatographic separation was performed on YMC-pack pro C(18), 50 x 4.6 mm, S-3 Îm column with an isocratic mobile phase composed of 10mM ammonium formate (pH 4.

Development and validation of a sensitive bioanalytical method for the quantitative estimation of pantoprazole in human plasma samples by LC-MS/MS: application to bioequivalence study.

The present study aims at developing a simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of pantoprazole sodium (PS) in human plasma using pantoprazole D3 (PSD3) as internal standard (IS). Chromatographic separation was performed on Zorbax SB-C18, 4.6 mm x 75 mm, 3.

GC-MS analysis, antibacterial activity and genotoxic property of Erigeron mucronatus essential oil.

GC-MS analysis of the hydrodistilled oil of Erigeron mucronatus DC was carried out, after the separation of a crystalline compound, lachnophyllum cumulene. Twenty-four components were identified. The oil contained 59.

Hptlc method development and validation of trandolapril in bulk and pharmaceutical dosage forms.

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and completely validated for the estimation of trandolapril in bulk and pharmaceutical dosage forms. Quantification of trandolapril was carried out with percolated silica gel 60F(254) as stationary phase using mobile phase consisting of Chloroform: Methanol: Acetic acid (8:1.5:0.

Large substituent effect on the photochemical rearrangement of 1,6-(N-Aryl)aza-[60]fulleroids to 1,2-(N-Arylaziridino)-[60]fullerenes.

Large substituent effects were observed in the rates and reaction mechanisms of the photochemical rearrangement of N-arylaza-[60]fulleroid 1 to N-arylaziridino-[60]fullerene 2, in which the difference of the rates between the fastest and the slowest (>2160-fold) was attained only by changing the aryl group from 1-naphthyl to 2-naphthyl. The decreasing order of the reaction rates in relation to the substituents was 1-naphthyl (1b) > 1-pyrenyl (1d) > phenyl (1a) > 2-naphthyl (1c). The reactions proceeded via triplet states of the fulleroids and a triplet sensitization of the reaction by rearranged product 2b was observed in the case of 1b.