Phosphorylation of auxin signaling repressor IAA8 by heat-responsive MPKs causes defective flower development.

Heat stress is a substantial and imminent threat to plant growth and development. Understanding its adverse effects on plant development at the molecular level is crucial for sustainable agriculture. However, the molecular mechanism underlying how heat stress causes developmental defects in flowers remains poorly understood.

Identification of mitogen-activated protein kinases substrates in using kinase client assay.

Mitogen-activated protein kinase (MPK) cascades are essential signal transduction components that control a variety of cellular responses in all eukaryotes. MPKs convert extracellular stimuli into cellular responses by the phosphorylation of downstream substrates. Although MPK cascades are predicted to be very complex, only limited numbers of MPK substrates have been identified in plants.

Quercetin induces pathogen resistance through the increase of salicylic acid biosynthesis in .

Quercetin is a flavonol belonging to the flavonoid group of polyphenols. Quercetin is reported to have a variety of biological functions, including antioxidant, pigment, auxin transport inhibitor and root nodulation factor. Additionally, quercetin is known to be involved in bacterial pathogen resistance in through the transcriptional increase of () genes.

Final amplifier of an ultra-intense all-OPCPA system with 13-J output signal energy and 41% pump-to-signal conversion efficiency.

Optical parametric chirped-pulse amplification (OPCPA) using high-energy Nd:glass lasers has the potential to produce ultra-intense pulses (>10 W/cm). We report on the performance of the final high-efficiency amplifier in an OPCPA system based on large-aperture (63 × 63-mm) partially deuterated potassium dihydrogen phosphate (DKDP) crystals. The seed beam (180-nm bandwidth, 110 mJ) was provided by the preceding OPCPA stages.

ROS-mediated plasmodesmal regulation requires a network of an Arabidopsis receptor-like kinase, calmodulin-like proteins, and callose synthases.

Plasmodesmata (PD) play a critical role in symplasmic communication, coordinating plant activities related to growth & development, and environmental stress responses. Most developmental and environmental stress signals induce reactive oxygen species (ROS)-mediated signaling in the apoplast that causes PD closure by callose deposition. Although the apoplastic ROS signals are primarily perceived at the plasma membrane (PM) by receptor-like kinases (RLKs), such components involved in PD regulation are not yet known.

Phosphorylation of the auxin signaling transcriptional repressor IAA15 by MPKs is required for the suppression of root development under drought stress in Arabidopsis.

Since plants are sessile organisms, developmental plasticity in response to environmental stresses is essential for their survival. Upon exposure to drought, lateral root development is suppressed to induce drought tolerance. However, the molecular mechanism by which the development of lateral roots is inhibited by drought is largely unknown.

Effect of the pump beam profile and wavefront on the amplified signal wavefront in optical parametric amplifiers.

We present a theoretical and experimental analysis of the signal phase introduced by the pump-beam wavefront and spatial profile during optical parametric amplification (OPA) process. The theory predicts the appearance of an additional wavefront in the amplified signal beam that is proportional to the spatial derivative of the pump-beam wavefront. The effect of the pump-beam profile on the signal-beam wavefront is also investigated.

Advanced laser development and plasma-physics studies on the multiterawatt laser.

The multiterawatt (MTW) laser, built initially as the prototype front end for a petawatt laser system, is a 1053 nm hybrid system with gain from optical parametric chirped-pulse amplification (OPCPA) and Nd:glass. Compressors and target chambers were added, making MTW a complete laser facility (output energy up to 120 J, pulse duration from 20 fs to 2.8 ns) for studying high-energy-density physics and developing short-pulse laser technologies and target diagnostics.

Analytic phase solutions of three-wave interactions.

Closed-form analytic phase solutions of three-wave interactions are presented for the first time, to the best of my knowledge. Cases from simple second-harmonic generation to the most general three-wave interactions without any constraints are considered. The phase amplification or deamplification behavior in the phase-sensitive parametric process is illustrated using the results obtained here.

Naringenin Induces Pathogen Resistance Against Through the Activation of NPR1 in .

Flavonoids are well known for the coloration of plant organs to protect UV and ROS and to attract pollinators as well. Flavonoids also play roles in many aspects of physiological processes including pathogen resistance. However, the molecular mechanism to explain how flavonoids play roles in pathogen resistance was not extensively studied.

A Gain-of-Function Mutant of IAA15 Inhibits Lateral Root Development by Transcriptional Repression of Genes in Arabidopsis.

Lateral root development is known to be regulated by Aux/IAA-ARF modules in . As components, several Aux/IAAs have participated in these Aux/IAA-ARF modules. In this study, to identify the biological function of IAA15 in plant developments, transgenic plant overexpressing the gain-of-function mutant of IAA15 (IAA15 OX) under the control of dexamethasone (DEX) inducible promoter, in which IAA15 protein was mutated by changing Pro-75 residue to Ser at the degron motif in conserved domain II, was constructed.

The Auxin Signaling Repressor IAA8 Promotes Seed Germination Through Down-Regulation of Transcription in .

Seed germination is a complex biological process controlled by various regulators, including phytohormones. Among these, abscisic acid and gibberellic acid inhibit and promote seed germination, respectively. Many studies have addressed the biological roles of auxin in plant growth and development, but very few have considered its role in seed germination.

Design and alignment of an all-spherical unobscured four-mirror image relay for an ultra-broadband sub-petawatt laser.

We describe the design and alignment of an unobscured reflective image relay for use in an ultra-broadband half-petawatt laser system in development at the University of Rochester's Laboratory for Laser Energetics. The design consists of four spherical mirrors in an unobscured configuration. We show the theoretical basis for such a four-mirror design using first-order optical matrix methods and nodal aberration theory.

Nonsurgical treatments for patients with radicular pain from lumbosacral disc herniation.

Background Context: Lumbosacral disc herniation (LDH) is one of the most frequent musculoskeletal diseases causative of sick leave in the workplace and morbidity in daily activities. Nonsurgical managements are considered as first line treatment before surgical treatment.

Purpose: This clinical practice guideline (CPG) is intended to provide physicians who treat patients diagnosed with LDH with a guideline supported by scientific evidence to assist in decision-making for appropriate and reasonable treatments.

Spatio-spectral characterization of broadband fields using multispectral imaging.

Spatio-temporal coupling in the field of ultrashort optical pulses is a technical enabler for applications but can result in detrimental effects such as increased on-target pulse duration and decreased intensity. Spectrally resolved spatial-phase measurements of a broadband field are demonstrated using a custom multispectral camera combined with two different wavefront sensors: a multilateral spatial shearing interferometer based on an amplitude checkerboard mask and an apodized imaged Hartmann sensor. The spatially and spectrally resolved phase is processed to quantify the commonly occurring pulse-front tilt and radial group delay, which are experimentally found to be in good agreement with models.

Gaussian curvature and stigmatic imaging relations for the design of an unobscured reflective relay.

We derive the relationship between Coddington's equations and the Gaussian curvature for a stigmatic reflective imaging system. This relationship allows parameterizing off-axis conic optical systems using traditional first-order optics by considering the effective curvature at the center of the off-axis sections. Specifically, we demonstrate parameterizing the system requirements of a 2× achromatic image relay for a terawatt laser system.

Investigation of an apodized imaged Hartmann wavefront sensor.

Quantitative wavefront measurements are demonstrated using a Hartmann mask re-imaged onto a camera. The wavefront is reconstructed using standard algorithms applied to the difference of beamlet centroids determined from fluence distributions obtained for two different longitudinal locations of the mask. The wavefront of the optical wave in the object plane is measured independently of imaging-system collimation.

Comparison of clinical efficacy of transforaminal and caudal epidural steroid injection in lumbar and lumbosacral disc herniation: A systematic review and meta-analysis.

Background Context: Epidural steroid injection has been used to treat back or radicular pain from lumbar and lumbosacral disc herniation (LDH). However, the superiority of transforaminal injection (TFESI) to caudal injection (CESI) remains controversial.

Purpose: This systematic review and meta-analysis aimed to investigate whether TFESI was more useful than CESI for achieving clinical outcomes in patients with LDH.

Delayed formation of sterile abscess after zygomaticomaxillary complex fracture treatment with bioabsorbable plates.

We present a patient who showed a sterile abscess after facial bone fixation with bioabsorbable plates and screws. He had zygomaticomaxillary complex and periorbital fracture due to falling down. The displaced bones were treated by open reduction and internal fixation successfully using bioabsorbable plate system.

Chromatic diversity: a new approach for characterizing spatiotemporal coupling of ultrashort pulses.

Two-dimensional chromatic aberrations are characterized by a single-shot scheme based on a simultaneous measurement of chromatically diversified focal spots. The chromatic diversity is introduced by a 2-D grating with holographic defocus terms. The chromatic aberrations in the beam are either subtracted or added by the additional known chromatic aberrations in the grating, depending on the diffraction order.

Reverse-Flow Intrinsic Fasciocutaneous Island Flaps in Foot Reconstruction.

Reconstruction of soft tissue defects in the foot remains a challenge due to its specialized tissue for weightbearing and ambulation. Considering the principle of replacing "like with like," adjacent soft tissues would be a best option for a donor site. Although several kinds of reverse-flow island flaps for the lower leg have been well described, intrinsic foot reverse flow flaps have been rarely reported.

Phosphorylation of the transcriptional repressor MYB15 by mitogen-activated protein kinase 6 is required for freezing tolerance in Arabidopsis.

The expression of CBF (C-repeat-binding factor) genes is required for freezing tolerance in Arabidopsis thaliana. CBFs are positively regulated by INDUCER OF CBF EXPRESSION1 (ICE1) and negatively regulated by MYB15. These transcription factors directly interact with specific elements in the CBF promoters.

Anatomical Study of the Infraorbital Nerve and Surrounding Structures for the Surgery of Orbital Floor Fractures.

The infraorbital nerve (ION) can easily be damaged by orbital trauma and periorbital surgical manipulations, due to its abutment to the orbital floor. Anatomic variability of the ION and surrounding structures has infrequently been documented. The aim of this study is to give precise anatomical knowledge about the ION with surrounding structures, to avoid iatrogenic injury of the ION during periorbital procedures.

Idiopathic Synkinesis of the Facial Musculature: Oculo-Nasal, Oculo-Zygomatic, and Fronto-Nasal Synkinesis.

Facial synkinesis is the simultaneous contraction of the certain facial musculature accompanying a motion of designated muscle in the face. With the exception of rare afflictions that are congenital in origin, most patients develop as a sequel to facial nerve paralysis due to trauma, tumor, and surgical injury. As an idiopathic congenital form, oculo-nasal synkinesis which reveals co-contraction of orbicularis oculi and the compressor narium minor muscles which are innervated by separate branches of the facial nerve have been already reported.

Insect odorant receptor trafficking requires calmodulin.

Background: Like most animals, insects rely on their olfactory systems for finding food and mates and in avoiding noxious chemicals and predators. Most insect olfactory neurons express an odorant-specific odorant receptor (OR) along with Orco, the olfactory co-receptor. Orco binds ORs and permits their trafficking to the dendrites of antennal olfactory sensory neurons (OSNs), where together, they are suggested to form heteromeric ligand-gated non-selective cation channels.