Molecularly Imprinted Polymer Biosensor Based on Nitrogen-Doped Electrochemically Exfoliated Graphene/Ti CNT MXene Nanocomposite for Metabolites Detection.

Rapid and accurate quantification of metabolites in different bodily fluids is crucial for a precise health evaluation. However, conventional metabolite sensing methods, confined to centralized laboratory settings, suffer from time-consuming processes, complex procedures, and costly instrumentation. Introducing the MXene/nitrogen-doped electrochemically exfoliated graphene (MXene@N-EEG) nanocomposite as a novel biosensing platform in this work addresses the challenges associated with conventional methods, leveraging the concept of molecularly imprinted polymers (MIP) enables the highly sensitive, specific, and reliable detection of metabolites.

Graphdiyne nanosheet as a novel sensing platform for self-enhanced electrochemiluminescence of MOF enriched ruthenium (II) in the presence of dual co-reactants for detection of tumor marker.

Graphdiyne (GDY) is a new two-dimensional carbon material with high charge carrier mobility, excellent conductivity, more suitable band gap, and natural pores was introduced as a new electrochemiluminescent sensing platform. Herein, the metal organic framework (MOFs) used for enrichment of luminophore with grafting Ru(bpy)(phen-NH)(Ru-complex) and Ru-complex amine-rich nitrogen-doped carbon nanodots(Ru-NCNDs) via both encapsulating and external decoration and decoration of SmS QDs as coreactant. Then, the MOF enriched Ru-complex (Ru@MOF@NCNDs-Ru@SmS QD) located on a GDY modified ITO electrode developed as a novel and efficient ECL platform.

Ultrasensitive molecularly imprinted fluorescence sensor for simultaneous determination of CA125 and CA15-3 in human serum and OVCAR-3 and MCF-7 cells lines using Cd and Ni nanoclusters as new emitters.

In the clinical diagnosis of tumors, a single-marker immunoassay may lead to false results. Thus there is a need for an effective and valid method for the simultaneous measurement of multiple tumor markers. In this work, an efficient fluorescence immunosensor for the simultaneous measurement of CA125 and CA15-3 tumor markers was fabricated by utilizing the high selectivity of magnetic molecularly imprinted polymers (MMIPs) and the high sensitivity of a fluorescence (FL) method.

Ratiometric fluorescence resonance energy transfer aptasensor for highly sensitive and selective detection of Acinetobacter baumannii bacteria in urine sample using carbon dots as optical nanoprobes.

Development of sensitive and selective analytical method for accurate diagnosis of Acinetobacter baumannii (Ab) bacteria in biological samples is a challenge. Herein, we developed an ingenious ratiometric fluorescent aptasensor for sensitive and selective detection of (Ab) bacteria based on fluorescence resonance energy transfer (FRET) between ortho-phenylenediamines carbon dot (o-CD), nitrogen-doped carbon nanodots (NCND) as donor's species and graphene oxide (GO) as acceptor. NCND that assembled onto the edge of graphene oxide (GO) exhibited quenched photoluminescence emission, and with the absorption of the modified o-CD with aptamer (o-CD-ssDNA) onto the graphene oxide surface the fluorescence of o-CD was efficiently quenched.

A self-enhanced ECL-RET immunosensor for the detection of CA19-9 antigen based on Ru(bpy)(phen-NH) - Amine-rich nitrogen-doped carbon nanodots as probe and graphene oxide grafted hyperbranched aromatic polyamide as platform.

Electrochemiluminescence resonance energy transfer (ECL-RET) assay as an efficient analytical technique has aroused considerable interest in recognition and biosensing. In the present study, a novel self-enhanced ECL-RET of Ru(bpy)(phen-NH) as an efficient luminophore to the MoS nanosheets as effective quencher was designed for CA19-9 antigen analysis. Herein, the graphene oxide grafted hyperbranched aromatic polyamide (GO-HBP) with high loading ability for Ru-complex was used as the sensing platform, while amine-rich nitrogen-doped carbon nanodots (NCNDs) which covalently linked to Ru(bpy)(phen-NH) applied as co-reactant for enhancing of anodic ECL signal response.

Indirect determination of mercury(II) by using magnetic nanoparticles, CdS quantum dots and mercury(II)-binding aptamers, and quantitation of released CdS by graphite furnace AAS.

This work describes an aptamer based method for highly sensitive determination of Hg(II). A Hg(II)-binding ssDNA aptamer was linked to silica-coated magnetic nanoparticles (magNPs). Then, a conjugate composed of graphene and CdS quantum dots (Gr-CdS) was linked to the complementary ssDNA.

Highly sensitive bioaffinity electrochemiluminescence sensors: Recent advances and future directions.

Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL) has attracted much attention in various fields of analysis due to the potential remarkably high sensitivity, extremely wide dynamic range and excellent controllability. Electrochemiluminescence biosensor, by taking the advantage of the selectivity of the biological recognition elements and the high sensitivity of ECL technique was applied as a powerful analytical device for ultrasensitive detection of biomolecule. In this review, we summarize the latest sensing applications of ECL bioanalysis in the field of bio affinity ECL sensors including aptasensors, immunoassays and DNA analysis, cytosensor, molecularly imprinted sensors, ECL resonance energy transfer and ratiometric biosensors and give future perspectives for new developments in ECL analytical technology.

Synthesis and characterization of novel bithiazolidine derivatives-capped CdTe/CdS quantum dots used as a novel Hg fluorescence sensor.

In the present study, (E)-2,2'-(4,4'-dioxo-2,2'-dithioxo-2H,2'H-[5,5'-bithiazolylidene]-3,3'(4H,4'H)-diyl) bis(3-mercaptopropanoic acid) (DTM) as a new derivative of thiazolidine was synthesis and characterized for the detrtmination of Hg ions. Then, the CdTe@CdS QDs and DTM capped CdTe@CdS QDs were produced. The DTM-CdTe@CdS/QDs used as an effective fluorescence sensing material due to the selective interaction of DTM with Hg (II).

A molecularly imprinted electrochemiluminescence sensor for ultrasensitive HIV-1 gene detection using EuS nanocrystals as luminophore.

Development of simple, sensitive and specific method for human immunodeficiency virus (HIV) assays are urgently demand. In this study, we developed a novel molecularly imprinted polymer (MIP) electrochemiluminescence (MIP-ECL) sensor for the highly sensitive and selective HIV-1 gene detection using Europium sulfide nanocrystals (EsNCs) as signal producing compound. Here, the HIV aptamer as the template and o-phenylenediamine as the functional monomer, were electropolymerized directly on the surface of ITO electrode.

Solid surface fluorescence immunosensor for ultrasensitive detection of hepatitis B virus surface antigen using PAMAM/CdTe@CdS QDs nanoclusters.

In the present study, we constructed an ultrasensitive solid surface fluorescence-immunosensor based on highly luminescent CdTe@CdS-PAMAM structures as nanoprobe for determination of HBsAg by monitoring fluorescence intensity. This strategy was achieved by using PAMAM as a signal amplifier; the PAMAM dendrimer with the many functional amine groups can amplify the fluorescence signal of QDs by covalent attachment of CdTe@CdS on PAMAM and hence, improve the sensitivity of the proposed method significantly. A sandwich type immunosensor was formed after the addition of HBsAg and the PAMAM-QD-Ab, respectively.

Nickel nanoclusters as a novel emitter for molecularly imprinted electrochemiluminescence based sensor toward nanomolar detection of creatinine.

In this study nickel nanoclusters (NiNCs), was promised as novel and economic electrochemiluminescence (ECL) emitter for highly sensitive and selective determination of creatinine in the presence of molecularly imprinted polymer (MIP). The uniform magnetic graphene oxide (GO-FeO) MIP film was established on the surface of ITO electrode and Ni NCs-embedded in MIP, showed a strong anodic ECL emission using tri-n-propylamine (TPrA) as coreactant. During the ECL process, TPrA was oxidized, and Ni NCs got the energy to generate excited state Ni NCs* for light emission.

Switchable electrochemiluminescence aptasensor coupled with resonance energy transfer for selective attomolar detection of Hg via CdTe@CdS/dendrimer probe and Au nanoparticle quencher.

In the present study, an ultrasensitive electrochemiluminescence (ECL) aptasensing assay for selective detection of Hg was designed. In this electrochemiluminescence resonance energy transfer (ECL-RET) approach, FeO@SiO/dendrimers/QDs exhibited amplified ECL emissions (switch "on" state) and with the hybridization between T-rich ssDNA(S) immobilized on the FeO@SiO/dendrimers/QDs and AuNPs modified with complementary aptamer (AuNPs-S), the ECL of QDs nanocomposites was efficiently quenched (switch "off" state). In the presence of Hg ions, formation of strong and stable T-Hg-T complex led to the release of the AuNPs-S from double-stranded DNA(dsDNA) and the recovery of the ECL signal of QDs (second signal switch "on" state).

Ultrasensitive electrochemiluminescence immunoassay for simultaneous determination of CA125 and CA15-3 tumor markers based on PAMAM-sulfanilic acid-Ru(bpy) and PAMAM-CdTe@CdS nanocomposite.

A multiplex ultrasensitive electrochemiluminescence (ECL) immunoassay was developed for the simultaneous determination of two different tumor markers, cancer antigen 153 (CA 15-3) and cancer antigen 125 (CA 125) using polyamidoamine dendrimer-quantum dots (PAMAM-QDs) and PAMAM-sulfanilic acid-Ru(bpy) as the signal probes and FeO-SiO as a magnetic bead. The CdTe@CdS- QDs and Ru(bpy) at the presence of tripropyl amine (TPA) as coreactant generate ECL at an applied voltage of + 1.2V (vs Ag/AgCl) in two different wavelengths 500 and 620nm, respectively.