Screening Options in Autism Telediagnosis: Examination of TAP, M-CHAT-R, and DCI Concordance and Predictive Value in a Telediagnostic Model.

Tele-assessment of autism in early childhood has increased. However, it is unclear how autism screening tools (M-CHAT-R, DCI) function as part of tele-assessment and relate to a commonly used tele-assessment instrument, the TAP. 361 families from a clinically referred sample of children (mean age: 27.

De novo identification and targeted sequencing of SSRs efficiently fingerprints Sorghum bicolor sub-population identity.

Recent plant breeding studies of several species have demonstrated the utility of combining molecular assessments of genetic distance into trait-linked SNP genotyping during the development of parent lines to maximize yield gains due to heterosis. SSRs (Short Sequence Repeats) are the molecular marker of choice to determine genetic diversity, but the methods historically used to sequence them have been burdensome. The ability to analyze SSRs in a higher-throughput manner independent of laboratory conditions would increase their utility in molecular ecology, germplasm curation, and plant breeding programs worldwide.

Hydrogen-bonded Multilayers of Silk Fibroin: From Coatings to Cell-mimicking Shaped Microcontainers.

We present a novel type of all-aqueous non-ionic layer-by-layer films of silk fibroin with synthetic macromolecules and a natural polyphenol. We found the multilayer growth and stability to be strongly pH-dependent. Silk assembled with poly(methacrylic) and tannic acids at pH=3.

Fluorescent labeling of yeast.

This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.

Comparative study of large-mode holey and conventional fibers.

Little information exists regarding how large-mode holey fibers compare, in practical terms, with their conventional counterparts. We present what is to our knowledge the first experimental study of mode area and bend loss for a range of large-mode holey and conventional fibers. It is demonstrated here that large-mode holey fibers exhibit mode areas and bending losses that are comparable to those of conventional fibers at 1.

ACT-5 is an essential Caenorhabditis elegans actin required for intestinal microvilli formation.

Investigation of Caenorhabditis elegans act-5 gene function revealed that intestinal microvillus formation requires a specific actin isoform. ACT-5 is the most diverged of the five C. elegans actins, sharing only 93% identity with the other four.

Polarization mode dispersion reduction in spun large mode area silica holey fibres.

We report the fabrication of the first spun holey optical fibre. Our experiments show that the complex air/glass transverse structure can be retained when the preform is spun during the fibre drawing process. Measurements of differential group delay (DGD) confirm that significant reductions in polarization mode dispersion (PMD) can be readily achieved using this approach.

Structure and propagation of modes of large mode area holey fibers.

We report cross-section measurement and propagation measurement of modes of large mode area holey fibers using near-field scanning optical microscopy (NSOM). Mode profiles are measured at the fiber end face using a scanning optical fiber tip held 10 nm from the surface, and compared to theoretical models. Both amplitude and phase of the electric field of the propagating light is measured using NSOM techniques as a function of distance from the fiber end, from 10 nm to 150 microm.

The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae.

Clathrin-binding adaptors play critical roles for endocytosis in multicellular organisms, but their roles in budding yeast have remained unclear. To address this question, we created a quadruple mutant yeast strain lacking the genes encoding the candidate clathrin adaptors Yap1801p, Yap1802p, and Ent2p and containing a truncated version of Ent1p, Ent1DeltaCBMp, missing its clathrin-binding motif. This strain was viable and competent for endocytosis, suggesting the existence of other redundant adaptor-like factors.

Nonlinear femtosecond pulse compression at high average power levels by use of a large-mode-area holey fiber.

We demonstrate that nonlinear fiber compression is possible at unprecedented average power levels by use of a large-mode-area holey (microstructured) fiber and a passively mode-locked thin disk Yb:YAG laser operating at 1030 nm. We broaden the optical spectrum of the 810-fs pump pulses by nonlinear propagation in the fiber and remove the resultant chirp with a dispersive prism pair to achieve 18 W of average power in 33-fs pulses with a peak power of 12 MW and a repetition rate of 34 MHz. The output beam is nearly diffraction limited and is linearly polarized.

Clathrin function in yeast endocytosis.

The process of endocytosis is a complex series of events involving the coordinated activity of many proteins. In animal cells, clathrin plays a vital role in the invagination of the plasma membrane leading to formation of vesicles during endocytosis. The study of endocytosis in yeast cells has been hindered by a debate about the role of clathrin in early internalization steps.

The transfer of child patients from the community dental service to the general dental service.

Objective: To determine an effective strategy for the transfer of children from the CDS to the GDS.

Design: Single centre study comprising a retrospective analysis of a 50% random sample of dental records for children transferred from CDO to GDP.

Setting: Community dental clinic in an urban area of south Manchester with relatively little material deprivation.

Long-term health outcomes and medical effects of torture among US Navy prisoners of war in Vietnam.

Objective: To characterize incidence of illnesses and injuries from 1979 to 1993 in former naval aviator prisoners of war (POWs) from the Vietnam War and a comparison group of naval aviators from the same war.

Design: Cohort analytic study.

Setting: A US Navy primary care clinic.

Orthopedic injuries experienced by U.S. prisoners of war during Operation Desert Storm: a descriptive analysis.

U.S. prisoners of war from Operation Desert Storm suffered significant orthopedic injuries.

Compulsive personality traits affecting aeronautical adaptability in a naval aviator: a case report.

This is a case report of a male naval aviator who demonstrated compulsive personality traits which adversely affected the performance of his duties as a pilot trainee, and later, as a naval flight officer. The Diagnostic and Statistical Manual of the American Psychiatric Association (DSM-III-R) uses the term obsessive-compulsive to describe two conditions. A review of terminology is undertaken to present the reader with the distinguishing features of the personality disorder versus the anxiety disorder.

Brief reactive psychosis in naval aviation.

Five naval aviation personnel diagnosed with brief reactive psychosis (BRP) have appeared before the Special Board of Flight Surgeons (SBFS) at the Naval Aerospace Medical Institute (NAMI). These cases demonstrate the challenges in diagnosis and disposition that face the clinical aeromedical specialist in evaluating the aviator who develops psychiatric symptoms. We discuss the aeromedical factors that must be considered when contemplating returning aviators with psychiatric diagnoses to flying duties.

Inflight anxiety conditions presenting with "break-off" symptoms.

The psychological dimension of disorientation is explored through two experienced pilots who developed inflight anxiety conditions. These were associated with "break-off" symptoms, which are inflight sensations of separation from the ground, the environment, and the aircraft. Following extensive psychiatric and medical evaluations, one aviator was diagnosed as having agoraphobia without panic disorder, and the other as having an otherwise unspecified anxiety disorder.