[Xenon and sevoflurane anti stress activity comparative assessment during elective anaesthesia in pediatric patients].

Unlabelled: Research objective was to compare Xenon and Sevoflurane anti stress activities during elective anaesthesia in Pediatric patients.

Material And Methods: The results of anaesthesia in 42 patients in age from 1 to 18 years were analyzed. The clinical sings, BIS-index, Somatotropinum hormone and Cortisol levels in patient's blood were studied.

Cortical visual areas process intestinal information during slow-wave sleep.

Background: Previously we have shown that, during sleep, electrical and magnetic stimulation of areas of the stomach and small intestine evoked neuronal and EEG responses in various cortical areas. In this study we wanted to correlate natural myoelectrical activity of the duodenum with cortical neuronal activity, and to investigate whether there is a causal link between them during periods of slow-wave sleep.

Methods: We have recorded the myoelectrical activity from the wall of the duodenum and activity of single neurons from three cortical visual areas in naturally sleeping cats and investigated causal interrelationship between these structures during slow-wave sleep.

[Medical care provision to the victim children of Haiti].

Treatment and diagnostic measures, including anesthesia during catastrophes and natural disasters are a world known problem. The paper presents the experience of anesthetic care provision during specialized surgical care to the victim children of the earthquake in Haiti. The materials of analysis were 529 anesthesias conducted to 72 children, who were in the treatment for crush syndrome, skeletal and concomitant injury.

[Structural organization of neurons of spinal ganglia innervating the colon].

The localization and morphological features of viscerosensory neurons of sacral spinal ganglia (SSG), innervating the colon, were investigated. In urethane anaesthetized cats, the solution of horseradish peroxidase was injected under the serosa of ascending and descending parts of the colon as well as of the rectum. After 48 hours animals were repeatedly anesthetized and transcardially perfused.

Autonomic response and Fos expression in the NTS following intermittent vagal stimulation: importance of pulse frequency.

Chronic intermittent stimulation of the vagus nerve (VNS) is an approved adjunctive therapy of refractory epilepsy. Nevertheless, the circuits triggered by VNS under the variable conditions used in patients are not well understood. We analyzed the effect of increasing pulse frequency on physiological variables (intragastric pressure, cardiac and respiratory frequencies) and neuronal activation in the solitary tract nucleus (NTS), the entry level of peripheral vagal afferents, in the rat.

[Procedure for determining the initiation of tube feeding in children in an intensive care unit].

A procedure is proposed to determine the timing of tube feeding in children treated in an intensive care unit, which records the hypertonia of the pyloric sphincter and consists in the determination the time of gastric administration of distilled water before its evacuation into the duodenal lumen (water evacuation test) visualized in the real-time mode through abdominal ultrasonography. The proposed procedure was successfully applied to 42 children aged 3 months to 14 years who had severe brain injury, appendicular peritonitis, polytrauma, and polysegmental pneumonias. This determining procedure makes it possible to exclude radiation exposure in patients treated in intensive care units and to improve the outcomes of treatment, by prescribing the artificial feeding, that is adequate to the patients' status, in the periods optimal for each specific clinical case.

Visceral-related area in the rat insular cortex.

The insular cortex interacts with the bulbar autonomic nuclei providing autonomic manifestations accompanying several neurological and psychosomatic disorders. The aim of our study was to identify the sites within the insular cortex, which could be responsible for the gastrointestinal, respiratory and cardiovascular responses. The main methods used were microinjections of HRP into several parts of the bulbar dorsal vagal complex and microstimulation of the insular cortex.

Muscarinic modulation of nitrergic neurotransmission in guinea-pig gastric fundus.

Muscarinic receptor activation by (4-Hydroxy-2-butynyl)-1-trimethylammonium-m-chlorocarbanilate chloride (McN-A-343) was investigated both on NADPH-d staining and on electrically induced responses in guinea-pig gastric fundus. McN-A-343 (10 micromol L(-1)) significantly increased the optical density of NADPH-d positive neurones, while blockade of nitric oxide synthase with N(omega)-nitro-L-arginine (L-NA) decreased it, suggesting facilitation of nitric oxide (NO) production. Electrical field stimulation (EFS; 2 Hz, 0.

[The enteral feeding during the treatment of appendicular peritonitis in children].

A total of 65 patients, aged 3 to 14, with different forms of appendicular peritonitis, in whom the intestinal insufficiency syndrome (IIS), stage 2, was diagnosed, were investigated. 35 patients were in the main group, and 30 patients were in the control group; the subjects of the latter group had a similar form of peritonitis, i.e.

Amygdalofugal modulation of the vago-vagal gastric motor reflex in rat.

In experiments on urethane anaesthetized rats the influence of electrical stimulation of the central nucleus of the amygdala (CNA) on gastric motility and activity of gastric-related neurons of the dorsal vagal complex was studied. Stimulation of the CNA effected spontaneous gastric motility and caused both excitatory and inhibitory changes of vagal-induced gastric relaxation. The most significant effects, mainly inhibitory, were observed under stimulation of the medial CNA.

[Visceral field of the rat insular cortex].

Structural-functional organisation of the cortical insular area relating to processes of the visceral functions control, was analysed. Representation of gastrointestinal, respiratory, and cardiovascular systems in the area, is given. Sites of respective neuronal groups and specifics of their spatial organisation within the area, were found.

Identification of a respiratory related area in the rat insular cortex.

The aim of this study was to map areas within the rat insular cortex from which respiratory responses originate and compare those sites with gastrointestinal control regions. The insular cortex was systematically microstimulated and histological location of responsive sites determined. Increased inspiratory airflow and decreased respiratory cycle duration were considered to be respiratory excitatory responses.

[The neurons of the medial vestibular nucleus initiating the relaxation of the rat's stomach wall].

In acute rat experiments the technique of retrograde axonal transport of horseradish peroxidase in the medial vestibular nucleus allowed to identify a group of neurones sending axons to the "stomach" region of a single tract nucleus. These neurones and, accordingly, the descending vestibular/solitary links can be viewed as the morphologic basis for vestibular influences on the gastric motor activity. As was shown, local irritation of the neurones initiates relaxation of the stomach wall.

Gastric related neurons in the rat medial vestibular nucleus.

Some structural and functional peculiarities of the rat vestibular nuclei neurones involved in realisation of vestibular-gastrointestinal reflectory reactions were studied. After microinjection of a horseradish peroxidase solution in the 'gastric' area of the nucleus tractus solitarius, retrogradely-labelled cell bodies were found in caudal part of the medial vestibular nucleus. Electrical stimulation of these neurons resulted in the decrease of gastric tone.

[Possible mechanisms of the anterior limbic cortex influence on the neuron activity of the vago-solitary complex].

In ananesthetized cats, neurons of the nucleus of the tractus solitarius (NTS) and the dorsal motor nucleus of the vagus nerve (DMNV) revealed phasic excitatory responses to separate single vagal and cortical stimuli. Stimulation of the anterior limbic cortex combined with vagal stimulation resulted in inhibitory or excitatory modification of the vagal induced responses of the NTS and DMNV neurons. The data obtained suggest that complete inhibitory effects are related to general cortical mechanisms of control of the functional state of the brain stem visceral neurons.

[The dorsal motor nucleus of the vagus nerve and its role in innervating the digestive tract].

Findings on topography of dorsal motor vagal nucleus neurons from which axons towards functionally different regions of digestive tract extend were analysed. Based on that and on the data available in literature main peculiarities of these cells topical organization were formulated. It is noted that "gastrointestinal" neurons are localised in dorsal motor nucleus as narrow stripes oriented caudo-rostrad.

Identification of gastric related neurones in the rat insular cortex.

Location peculiarities of insular neurones implicated in the regulation of gastrointestinal motility have been studied in acute experiments on rats. After microinjection of a horseradish peroxidase solution in a part of the dorsal vagal complex that receive gastric afferent inputs, retrogradely labelled cell bodies are observed in a certain area of the agranular and disgranular insular cortex. Electrical stimulation of the insular cortex area had no significant effect on heart and respiration rate but had evoked gastric tone changes.

[The afferent link in the system of the bulbar regulation of gastric motor function].

The data obtained suggest a possible participation of neurons of the tractus solitarius' nucleus in mechanisms of the stomach motility control. These neurons seem to be involved in an afferent link of the bulbar "gastric" centre participating in regulation of the stomach motor activity.

Inhibition of gastric mechanoreceptor discharge by cholecystokinin in the rat.

The aim of the present study was to investigate electrophysiologically the effect of systemic cholecystokinin (CCK) on the discharge of vagal gastric mechanoreceptors. Twenty-two single vagal afferent fibers were selected for the investigation of responses to intravenous CCK octapeptide (CCK-8) on the basis of a positive response to gastric distension. Resting discharge in these afferent fibers was 1.

Limbic cortical influences to the vagal input neurones of the solitary tract nucleus.

In acute experiments on cats, electrical stimulation of the anterior limbic cortex was shown to modulate activity of vagal input neurones of the nucleus of the tractus solitarius. In the majority of the solitary tract nucleus neurones the responses induced by electrical stimulation of the vagus were depressed by the stimulation of anterior limbic cortex. Under these conditions the patterns of responses or their latency were changed.