[The endogenous differentiation factor of the HL-60 cells shows a nuclease activity].

A structural homology between the endogenous differentiation factor of the HL-60 cell line of promyelocyte leukemia (HLDF) and several DNA/RNA-binding and DNA/RNA-hydrolyzing proteins was revealed, and expression of the hldf gene in prokaryotic systems was studied. On the basis of these experiments, the amino acid sequence of an 8-membered fragment of HLDF with potential nuclease activity was identified. The synthetic octapeptide RRWHRLKE was shown to be capable of the cleavage of RNA, linear DNA from phage lambda, and all forms of plasmid DNA.

[Leukocyte agglomeration reaction in the diagnosis of allergic diseases].

This paper discusses the problem of how allergic responses to drug therapy can be obviated by using Flack's leukocyte agglomeration reaction (LAR). The sensitivity and reliability of the method were tested in patients who had drug allergic responses in their case histories. Altogether 112 patients were examined.