Rabies virus in the tonsils of a carrier dog.

A female dog, inoculated with a rabies isolate from the saliva of an apparently healthy Ethiopian dog, developed rabies but later recovered without supportive treatment. Rabies virus was isolated from the saliva collected 42, 169 and 305 days after recovery. Sixteen months after it recovered, the dog suddenly died after giving birth to two stillborn puppies.

Ploidy Effects in Isogenic Populations of Alfalfa : II. Photosynthesis, Chloroplast Number, Ribulose-1,5-Bisphosphate Carboxylase, Chlorophyll, and DNA in Protoplasts.

Photosynthetically-active protoplasts isolated from isogenic sets of diploid-tetraploid and tetraploid-octoploid alfalfa (Medicago sativa L.) leaves were used to investigate the consequences of polyploidization on several aspects related to photosynthesis at the cellular level. Protoplasts from the tetraploid population contained twice the amount of DNA, ribulose-1,5-bisphosphate carboxylase (RuBPCase), chlorophyll (Chl), and chloroplasts per cell compared to protoplasts from the diploid population.

Ploidy effects in isogenic populations of alfalfa : I. Ribulose-1,5-bisphosphate carboxylase, soluble protein, chlorophyll, and DNA in leaves.

The influence of polyploidization on ribulose-1,5-bisphosphate carboxylase (RuBPCase), buffer-soluble protein (BSP), chlorophyll (Chl), and DNA was examined in fully expanded leaves of isogenic diploid-tetraploid (DDC 2X-4X) and tetraploid-octoploid (IC 4X-8X) sets of alfalfa (Medicago sativa L.). The concentration of RuBPCase in leaf extracts was determined by rocket immunoelectrophoresis.

[Working conditions and complications during laryngomicroscopies under endotracheal ventilation via intubation tubes or injection ventilation].

During a period of 7 years 693 laryngomicroscopies (LAR) were performed on 490 patients. General anaesthesia was given by 21 anaesthesists: 5 applied ventilation only through a conventional intubation tube. Of the remaining 16 anaesthesists, 8 constantly prefered, 4 used occasionally jet ventilation (JET), and 4 applied both methods alternatively.

A simple and sensitive DNA assay for plant extracts.

A sensitive fluorimetric method was developed for the quantitative determination of DNA in plant (Zea mays L. and Medicago sativa L.) extracts.

Intradermal immunization with human diploid cell rabies vaccine. Serological and clinical responses of persons with and without prior vaccination with duck embryo vaccine.

Intradermal administration of human diploid cell rabies vaccine (HDCV) was investigated in an attempt to reduce the current high cost of preexposure rabies prophylaxis. The study population consisted of 240 volunteers from a veterinary hospital, 165 of whom had prior vaccination with duck embryo rabies vaccine (DEV). Vaccine safety was good; only minor reactions were experienced, all of which resolved spontaneously.

Excretion of rabies virus in the saliva of dogs.

Thirty-nine dogs were injected intramuscularly with either an Ethiopian strain or a Mexican strain of rabies virus. The excretion of rabies virus in the saliva was studied before and during illness. Nine of 17 dogs that died after injection with the Ethiopian strain had virus in the submaxillary glands.

Human diploid cell rabies vaccine. Effectiveness of immunization with small intradermal or subcutaneous doses.

To determine antibody responses to small doses of human diploid cell rabies vaccine (HDCV), we determined rabies antibody titers in 124 volunteers who had been vaccinated with one of five primary preexposure regimens. In a sixth group of 47 persons previously vaccinated with duck embryo rabies vaccine (DEV), we evaluated the booster responses after a 0.1-mL dose of intradermal (ID) HDCV.

Massive hemothorax in a child after removal of subclavian vein catheter.

A case is described where a near-fatal hemothorax developed in a child (age 13 months) right after the removal of the subclavian vein catheter. The catheter was introduced a week earlier and it had functioned without disorder. The possible mechanism of the complication is discussed.

Dual role of the immune response in street rabiesvirus infection of mice.

The cellular and humoral immune responses of mice to footpad injection of salivary gland suspensions of street rabiesvirus were investigated. Suppression of these responses with cyclophosphamide both increased the overall mortality rate from (50 to 100%) and delayed onset of disease signs and death for 1 to 2 weeks. Despite the absence of disease signs in these immunosuppressed animals, virus was present in the central nervous system, as shown by fluorescent-antibody staining of corneal epithelium.

In Vivo Determination of Parameters of Nitrate Utilization in Wheat (Triticum aestivum L.) Seedlings Grown with Low Concentration of Nitrate in the Nutrient Solution.

Six genotypes of winter wheat (Triticum aestivum L.) differing in grain protein concentration were grown on a nutrient solution containing low concentrations of NO(3) (-) (2 millimolar). Total NO(3) (-) uptake varied between genotypes but was not related to grain protein content.

[Documentation of central venous catheterization using a computer laboratory system (author's transl)].

Full documentation of every case of central venous catheterization is a prerequisite for reducing the incidence of failures and complications. A laboratory computer program already in use was modified for this purpose: the start of the operation is put in in "real time", abandoned or failed procedures or incomplete data are detected, and can be queried on the day of patient is discharged from hospital. Adverse effects are shown up at an early stage by the quarterly print-out.

Intermittent excretion of rabies virus in the saliva of a dog two and six months after it had recovered from experimental rabies.

A dog inoculated with a rabies virus isolate from the saliva of an apparently healthy Ethiopian dog was followed for more than 9 months. Saliva and blood specimens were collected three times weekly and cerebrospinal fluid weekly. Saliva samples collected on days 42 and 169 after the dog's recovery produced fatal rabies infections in mice inoculated intracerebrally.

[Our experiences during cannulation of the subclavian vein in children (author's transl)].

With 260 children under 12 years of age we tried to cannulate the subclavian vein during 3 years 455 times, and were successful 391 times (85.9%). 43.

[Ketamine-induced psychopathological changes in normal volunteers during conditions used for experimental psychoses (author's transl)].

The psychotomimetic properties of ketamine were studied in 9 physically and psychically normal medical students and nurses during 20 single-blind experiments. During the experiments any disturbance to the subject was avoided. A dose of 0.

Rapid antibody response to human diploid rabies vaccine.

The speed of antibody response to two administration schedules of human diploid cell strains rabies vaccine (HDCV) was measured in two groups of veterinary students as a preliminary study to the use of interferon inducers plus vaccine for rabies treatment. When four doses of HDCV were administered in three days instead of four doses in 14 days, 94.7 per cent of those injected had antibody by the seventh day, versus 31.

Rabies serum neutralizing antibody in mongooses from Grenada.

During a four-year study on Grenada, 4,754 mongooses were examined, of which 100 (2.1%) were rabid. Of 1,675 mongooses tested for rabies serum neutralizing (SN) antibody, 498 (30%) were positive.

Minimal interferon induction in dogs, using a modified polyriboinosinic-polyribocytidylic acid complex.

Adult Beagles failed to respond to high concentrations of interferon (IF) when they were injected with a nuclease-resistant complex poly I:C with poly-L-lysine and carboxymethylcellulose (PICLC), by the IV or intrathecal route. An IV dose of 1 mg of PICLC/kg of body weight was lethal to 1 of 3 adult dogs, but induced IF in only 2 dogs. Smaller doses were less toxic, but also were less effective.

Recovery from clinical rabies of 2 dogs inoculated with a rabies virus strain from Ethiopia.

Two dogs, inoculated with a strain of rabies virus from Ethiopia, showed typical signs of rabies 8 days after inoculation. After 3 or 4 days with a deterioration in the physical condition, both animals began to recover, as shown by increased muscular movements, reaction to stimuli, awareness of surroundings, and attempts to rise. Both animals recovered completely, although 1 then died of Pseudomonas bacterial pneumonia.

Experimental rabies in the vampire bat.

Vampire bats were inoculated intramuscularly and subcutaneously with varying doses of rabies virus to simulate bites by rabid animals in nature. Daily saliva samples were then taken from these animals to determine whether they excreted virus and for how long. Vampire bats appear to react to rabies virus as do other animals, with variable incubation periods, some excretion of virus in the saliva, but no prolonged excretion "carrier state.