Vibrational fiber photometry: label-free and reporter-free minimally invasive Raman spectroscopy deep in the mouse brain.

Optical approaches to monitor neural activity are transforming neuroscience, owing to a fast-evolving palette of genetically encoded molecular reporters. However, the field still requires robust and label-free technologies to monitor the multifaceted biomolecular changes accompanying brain development, aging or disease. Here, we have developed vibrational fiber photometry as a low-invasive method for label-free monitoring of the biomolecular content of arbitrarily deep regions of the mouse brain in vivo through spontaneous Raman spectroscopy.

Closing the Sex-Based Differences in Stroke Care: Insights from a Large Telestroke Network on Treatment and Postacute Management.

Background: The evolution of ischemic stroke is different accordin'g to sex and is one of the main causes of death in women. Previous studies have shown that women are less likely to receive acute treatment, and stroke center type is an important predictor of door-to-needle times. We investigated whether women are attended in a similar way to men in the telestroke network with specialized stroke physicians.

Machine learning identifies experimental brain metastasis subtypes based on their influence on neural circuits.

A high percentage of patients with brain metastases frequently develop neurocognitive symptoms; however, understanding how brain metastasis co-opts the function of neuronal circuits beyond a tumor mass effect remains unknown. We report a comprehensive multidimensional modeling of brain functional analyses in the context of brain metastasis. By testing different preclinical models of brain metastasis from various primary sources and oncogenic profiles, we dissociated the heterogeneous impact on local field potential oscillatory activity from cortical and hippocampal areas that we detected from the homogeneous inter-model tumor size or glial response.

Protocol to generate murine organotypic brain cultures for drug screening and evaluation of anti-metastatic efficacy.

Organotypic brain cultures are short-term assays that phenotypically and functionally recapitulate brain metastatic cells in vivo. Here, we present a protocol to generate murine organotypic brain cultures for drug screening. We describe steps for sectioning of murine brains and plating of organotypic cultures.

Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.

Whole-brain radiotherapy (WBRT) is the treatment backbone for many patients with brain metastasis; however, its efficacy in preventing disease progression and the associated toxicity have questioned the clinical impact of this approach and emphasized the need for alternative treatments. Given the limited therapeutic options available for these patients and the poor understanding of the molecular mechanisms underlying the resistance of metastatic lesions to WBRT, we sought to uncover actionable targets and biomarkers that could help to refine patient selection. Through an unbiased analysis of experimental in vivo models of brain metastasis resistant to WBRT, we identified activation of the S100A9-RAGE-NF-κB-JunB pathway in brain metastases as a potential mediator of resistance in this organ.

Criterion-related validity of field-based muscular fitness tests in youth.

Aim: The purpose of the present study was to analyze the association between isokinetic strength and field-based muscular fitness tests in youth.

Methods: One hundred twenty-six adolescents (14.4±1.

Delayed diagnosis of fatigue fractures in the elderly.

A retrospective study was made of 30 stress fractures in elderly patients engaged in noncompetitive sporting activities, mostly marching and running. Fractures were located exclusively in the lower limbs. In many cases the diagnosis was delayed and in several cases was incorrect because the stress fracture was mistaken for other conditions.

Massive burns: a study of epidemiology and mortality.

This study describes the epidemiology and mortality of patients with burns involving over 30 per cent of the body surface who were hospitalized between 1 January 1988 and 1 January 1990. During this period 302 patients were hospitalized (8.1 per cent of those treated); of these, 52 (16.

Application of transcutaneous PO2 determinations for the postoperative monitoring of skin grafts.

A study of transcutaneous PO2 measurements was made in 25 patients with partial thickness skin grafts applied to different full skin thickness burn areas. Transcutaneous oximetry was performed 4, 7, 15, 22, 30, 40, 50, 60, 75 and 90 days postgrafting, with the aim of offering a quantifiable diagnostic method for the postoperative monitoring of skin grafts, as a complement to the classical approach of observation and clinical evaluation. An increase in PO2 was detected following grafting--the greatest increase was noted after day 8 postgrafting, and possibly coincides with the establishment of the vascular plexus from the combined underlying bed and graft.

An epidemiological study of burn patients hospitalized in Valencia, Spain during 1989.

A retrospective study has been made of the patients hospitalized in the Burn Centre of La Fe Hospital in Valencia (Spain) during 1989. Of the 1825 patients seen during this period, 146 (8 per cent) were admitted to hospital. The mean patient age was 31.