[Simple, highly specific procedures for radioimmunochemical estimation of hypophyseal protein hormones].

Immunosorbents, prepared by means of polymerization of antiserum proteins using chlorcarbonic acid isobutyl ester, were used to increased reproducibility and simplicity of the radioimmunochemical procedures for estimation of the estimation of the hypophyseal hormones. In the types of the radioimmunoassay (RIA) studied the immunosorbents were prepared by means of polymerization of the first and second antibodies. The universal immunosorbent, suitable to estimation of various antigens, was prepared from the second antibodies.

[Bovine epiphyseal lactotropin, biologically and immunochemically related to pituitary prolactin].

A substance biologically and immunologically related to the hypophysis lactogenic hormone (HLH) was detected in a purified preparation obtained by extracting the bovine pineal glands (epiphyses) with acidified acetonic saline fractionation, precipitation in isoelectric point and gel filtration. Bioactivity of the pineal gland lactogen was evaluated according to its ability for stimulating the pigeon thyroid gland proliferation. Equal doses of the pineal gland hormone and HLH produced the same biological effect.

[Extrapituitary localization of luteinizing hormone in rats].

After hypophysectomy luteinizing hormone /LH/ did not disappear from rat blood serum within 2 months; but concentration of the hormone decreased 2-fold. Administration of luliberine into the hypophysectomized rats led to approximately 3-fold increase of the LH concentration in blood serum within 20 min. The hormones from blood serum of the hypophysectomized rats, from hypophysis and blood serum of intact rats exhibited similarity in their immunochemical and chromatographic properties.

[Luteinizing hormone content in the pineal body depending on the physiological state of rats].

The changes in the luteinizing hormone (LH) level in the pineal gland were studied in rats depending on their sex and age, and under conditions of hypophysectomy or gonadectomy. In golden hamsters these changes were studied during the sexual cycle. The LH level was determined by the radioimmunological method.

[Gonadotropin from the bovine pineal body. Evidence of its similarity with the luteinizing hormone of the pituitary gland].

Gonadotropin affiliated by biological and immunochemical properties to the luteinizing hormone of the hypophysis was revealed in the purified extracts from the bovine pineal glands. Biological properties of gonadotropin from the pineal glands were assessed by the reactions specific of the luteinizing hormone. Immunochemical properties of gonadotropin were studied by means of gel-precipitation in agar gel, immunoelectrophoresis and radioimmunochemical analysis.